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Product Info Summary
| SKU: | A03572-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PLCG 2/PLCG2 Antibody Picoband®
View all PLC-gamma 2 Antibodies
SKU/Catalog Number
A03572-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PLCG 2/PLCG2 Antibody Picoband® catalog # A03572-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PLCG 2/PLCG2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03572-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human PLCG 2 recombinant protein (Position: R1201-V1258).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03572-1 is reactive to PLCG2 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
148 kDa
Calculated molecular weight
147.87kDa
Background of PLC-gamma 2
1-Phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-2 is an enzyme that in humans is encoded by the PLCG2 gene. The protein encoded by this gene is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to 1D-myo-inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor. IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03572-1 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot,0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section),2-5μg/ml
Immunocytochemistry/Immunofluorescence, 5 μg/ml
Flow Cytometry (Fixed), 1-3 μg/1x106 cells
ELISA,0.1-0.5μg/ml
Positive Control
WB: human Raji whole cell, human Daudi whole cell
IHC: human tonsil tissue
ICC/IF: U20S cell
FCM: THP-1 cell
Validation Images & Assay Conditions
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Figure 4. Flow Cytometry analysis of THP-1 cells using anti-PLCG 2/PLCG2 antibody (A03572-1).
Overlay histogram showing THP-1 cells stained with A03572-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PLCG 2/PLCG2 Antibody (A03572-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Figure 1. Western blot analysis of PLCG 2/PLCG2 using anti-PLCG 2/PLCG2 antibody (A03572-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysates,
Lane 2: human Daudi whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLCG 2/PLCG2 antigen affinity purified polyclonal antibody (Catalog # A03572-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLCG 2/PLCG2 at approximately 148 kDa. The expected band size for PLCG 2/PLCG2 is at 148 kDa.
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Figure 2. IHC analysis of PLCG 2/PLCG2 using anti-PLCG 2/PLCG2 antibody (A03572-1).
PLCG 2/PLCG2 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PLCG 2/PLCG2 Antibody (A03572-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 3. IF analysis of PLCG 2/PLCG2 using anti-PLCG 2/PLCG2 antibody (A03572-1).
PLCG 2/PLCG2 was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PLCG 2/PLCG2 Antibody (A03572-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For PLCG2 (Source: Uniprot.org, NCBI)
Gene Name
PLCG2
Full Name
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2
Weight
147.87kDa
Alternative Names
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2; Phosphoinositide phospholipase C-gamma-2; Phospholipase C-IV; PLC-IV; Phospholipase C-gamma-2; PLC-gamma-2; PLCG2 PLCG2 APLAID, FCAS3, PLC-IV, PLC-gamma-2 phospholipase C gamma 2 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2|phosphoinositide phospholipase C-gamma-2|phospholipase C, gamma 2 (phosphatidylinositol-specific)|phospholipase C-IV
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PLCG2, check out the PLCG2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PLCG2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PLCG 2/PLCG2 Antibody Picoband® (A03572-1)
Hello CJ!
A03572-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Enhancement or inhibition of PLCγ2 expression in rat hepatocytes by recombinant adenoviral vectors that contain full-length gene or siRNA
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Customer Reviews
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Customer Q&As
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17 Customer Q&As for Anti-PLCG 2/PLCG2 Antibody Picoband®
Question
We have been able to see staining in rat leukemic t-cell. Do you have any suggestions? Is anti-PLCG 2/PLCG2 antibody supposed to stain leukemic t-cell positively?
Verified Customer
Verified customer
Asked: 2020-03-31
Answer
From what I have seen in literature leukemic t-cell does express PLCG2. From what I have seen in Uniprot.org, PLCG2 is expressed in renal glomerulus, lymphoblast, spleen, lymph, leukemic t-cell, among other tissues. Regarding which tissues have PLCG2 expression, here are a few articles citing expression in various tissues:
Leukemic T-cell, Pubmed ID: 15144186, 19690332
Lymph, Pubmed ID: 15489334
Lymphoblast, Pubmed ID: 2849563
Spleen, Pubmed ID: 16533400
Boster Scientific Support
Answered: 2020-03-31
Question
I was wanting to use to test anti-PLCG 2/PLCG2 antibody A03572-1 on rat lymph for research purposes, then I may be interested in using anti-PLCG 2/PLCG2 antibody A03572-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-16
Answer
The products we sell, including anti-PLCG 2/PLCG2 antibody A03572-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-16
Question
Will anti-PLCG 2/PLCG2 antibody A03572-1 work for WB with lymph?
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
According to the expression profile of lymph, PLCG2 is highly expressed in lymph. So, it is likely that anti-PLCG 2/PLCG2 antibody A03572-1 will work for WB with lymph.
Boster Scientific Support
Answered: 2020-02-20
Question
Do you have a BSA free version of anti-PLCG 2/PLCG2 antibody A03572-1 available?
Verified Customer
Verified customer
Asked: 2019-08-26
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-PLCG 2/PLCG2 antibody A03572-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-08-26
Question
What is the molar concentration of A03572-1 antibody?
Verified customer
Asked: 2019-08-20
Answer
The molar concentration of the Anti-PLCG 2/PLCG2 Antibody Picoband A03572-1 is 6.6667 uM.
Boster Scientific Support
Answered: 2019-08-21
Question
Can A03572-1 be conjugated with Biotin.
Verified customer
Asked: 2019-06-26
Answer
Yes, the Anti-PLCG 2/PLCG2 Antibody Picoband™ (A03572-1) can be conjugated with Biotin.
Boster Scientific Support
Answered: 2019-06-27
Question
Would A03572-1 anti-PLCG 2/PLCG2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-13
Answer
As indicated on the product datasheet, A03572-1 anti-PLCG 2/PLCG2 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-13
Question
We are currently using anti-PLCG 2/PLCG2 antibody A03572-1 for human tissue, and we are satisfied with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?
J. Miller
Verified customer
Asked: 2019-05-01
Answer
The anti-PLCG 2/PLCG2 antibody (A03572-1) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-01
Question
We ordered your anti-PLCG 2/PLCG2 antibody for IHC on renal glomerulus in the past. I am using human, and I plan to use the antibody for WB next. My lab would like examining renal glomerulus as well as leukemic t-cell in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
F. Jones
Verified customer
Asked: 2019-02-22
Answer
I took a look at the website and datasheets of our anti-PLCG 2/PLCG2 antibody and I see that A03572-1 has been validated on human in both IHC and WB. Thus A03572-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-02-22
Question
I have attached the WB image, lot number and protocol we used for lymph using anti-PLCG 2/PLCG2 antibody A03572-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-12-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-12-17
Question
Is this A03572-1 anti-PLCG 2/PLCG2 antibody reactive to the isotypes of PLCG2?
R. Taylor
Verified customer
Asked: 2018-11-29
Answer
The immunogen of A03572-1 anti-PLCG 2/PLCG2 antibody is E. coli-derived human PLCG 2 recombinant protein (Position: R1201-V1258). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-11-29
Question
I was wanting to use your anti-PLCG 2/PLCG2 antibody for WB for rat lymph on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat lymph identification?
E. Bhatt
Verified customer
Asked: 2018-04-12
Answer
You can see on the product datasheet, A03572-1 anti-PLCG 2/PLCG2 antibody has been validated for ELISA, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat lymph in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-04-12
Question
I am interested in using your anti-PLCG 2/PLCG2 antibody for dap12 signaling studies. Has this antibody been tested with western blotting on placenta tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-04-04
Answer
Thanks for your inquiry. This A03572-1 anti-PLCG 2/PLCG2 antibody is validated on human tonsil tissue, placenta tissue, mouse spleen tissue, intestinal cancer tissue, rat thymus tissue. It is guaranteed to work for ELISA, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-04-04
Question
I see that the anti-PLCG 2/PLCG2 antibody A03572-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-12-18
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-12-18
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lymph using anti-PLCG 2/PLCG2 antibody A03572-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-12-12
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-12-12
Question
I have a question about product A03572-1, anti-PLCG 2/PLCG2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-08-14
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03572-1 anti-PLCG 2/PLCG2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-08-14
Question
Is a blocking peptide available for product anti-PLCG 2/PLCG2 antibody (A03572-1)?
K. Parker
Verified customer
Asked: 2014-03-10
Answer
We do provide the blocking peptide for product anti-PLCG 2/PLCG2 antibody (A03572-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-03-10
