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Product Info Summary
| SKU: | M00001-4 |
|---|---|
| Size: | 200µg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, IHC, ICC, WB |
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Product info
Product Name
Anti-p53 Monoclonal Antibody (PAb122)
SKU/Catalog Number
M00001-4
Size
200µg
Form
Liquid
Description
Boster Bio Anti-p53 Monoclonal Antibody (PAb122) (Catalog# M00001-4). Tested in Flow Cytometry, ICC, IHC, IP, WB application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Cite This Product
Anti-p53 Monoclonal Antibody (PAb122) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00001-4)
Host
Mouse
Contents
Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
Clonality
Monoclonal
Clone Number
pAb122
Isotype
IgG2b
Immunogen
SV40 transformed 3T3 cells
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00001-4 is reactive to Tp53 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
83786 MW
Background of p53
Anti-Glycogen Synthase 1 pS641 antibody is validated by IHC, Western Blot and ELISA. Human muscle glycogen synthase (GS) is responsible for the biosynthesis of glycogen from phosphorylated glucose units. Mammalian liver and muscle contain GS consisting of four subunits with a total molecular weight of 360,000. GS is subject to regulation through both allosteric and covalent modification and occurs in two forms: the phosphorylated inactive form, and the dephosphorylated active form. GS is inactivated by the serine/threonine kinase called glycogen synthase kinase-3 that mainly functions to phosphorylate muscle glycogen synthase. This antibody is specific for the phosphorylated form of GS at S641. Phosphorylation of GS at S641 has been associated with Antiphospholipid Antibody Syndrome.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00001-4 is guaranteed for Flow Cytometry, IP, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot (1:250 colorimetric, 1:250 ECL). Detects a band of ~48/53kDa.
Immunohistochemistry (1:20 - 1:100)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Tp53 using anti-Tp53 antibody (M00001-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tp53 antigen affinity purified polyclonal antibody (Catalog # M00001-4) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1021) with Tanon 5200 system. A specific band was detected for Tp53.
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Figure 2. IHC analysis of Tp53 using anti-Tp53 antibody (M00001-4).
Tp53 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Tp53 Antibody (M00001-4) overnight at 4°C. Biotinylated goat anti Mouse IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
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Figure 3. IHC analysis of Tp53 using anti-Tp53 antibody (M00001-4).
Tp53 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Tp53 Antibody (M00001-4) overnight at 4°C. Biotinylated goat anti Mouse IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Tp53 (Source: Uniprot.org, NCBI)
Gene Name
Tp53
Full Name
Cellular tumor antigen p53
Weight
83786 MW
Superfamily
p53 family
Alternative Names
muscle glycogen synthase pS641, glycogen synthase pS641, Glycogen, Glycogen synthase 1 (muscle), Glycogen synthase 1, Glycogen synthase1, GYS 1, GYS-1, GYS1, Starch synthase muscle TP53 BCC7, BMFS5, LFS1, P53, TRP53 tumor protein p53 cellular tumor p53| NY-CO-13|mutant tumor protein 53|p53 tumor suppressor|phosphoprotein p53|transformation-related protein 53|tumor protein 53|tumor supressor p53
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Tp53, check out the Tp53 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Tp53: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-p53 Monoclonal Antibody (PAb122) (M00001-4)
Hello CJ!
M00001-4 has been cited in 18 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Han Y,Chen M,Wang H. Production of a SCID mouse model of medulloblastoma to explore the therapeutic value of targeting tumor driver genes.Exp Ther Med.2021 Feb;21(2):108.doi: 10.3892/etm.2020.9540.Epub 2020 Nov 27.PMID: 33335571;PMCID:PMC7739861.
Species: Human, Mouse
M00001-4 usage in article: APP:IHC, SAMPLE:HUMAN SUBCUTANEOUS TUMOR AND MOUSE SUBCUTANEOUS TUMOR, DILUTION:1:1000
P-glycoprotein Mediates Postoperative Peritoneal Adhesion Formation by Enhancing Phosphorylation of the Chloride Channel-3
Effects of human umbilical cord mesenchymal stem cell transplantation combined with minimally invasive hematoma aspiration on intracerebral hemorrhage in rats.
Lactotransferrin expression is downregulated and affects the mitogen-activated protein kinase pathway in gastric cancer
Correlation ofp53gene mutation and expression of P53 protein in cholangiocarcinoma
Suspension culture combined with chemotherapeutic agents for sorting of breast cancer stem cells
A novel polysaccharide from Sargassum integerrimum induces apoptosis in A549 cells and prevents angiogensis in vitro and in vivo
TALENs-directed?knockout?of the?full-length?transcription?factor?Nrf1? that?represses?malignantbehaviour?of?human?hepatocellular?carcinoma?(HepG2)?cells
Effect of ligustilide on Ang II-induced hypertrophy in cardiomyocytes and the potential mechanisms
Feng D, Wu J, Tian Y, Zhou H, Zhou Y, Hu W, Zhao W, Wei H, Ling B, Ma C. Plos One. 2013 Nov 19;8(11):E80657. Doi: 10.1371/Journal.Pone.0080657. Ecollection 2013. Targeting Of Histone Deacetylases To Reactivate Tumour Suppressor Genes And Its Thera...
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-p53 Monoclonal Antibody (PAb122)
Question
Does M00001-4 anti-p53 Monoclonal antibody (PAb122) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-18
Answer
It shows on the product datasheet, M00001-4 anti-p53 Monoclonal antibody (PAb122) as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-18
Question
Do you have a BSA free version of anti-p53 Monoclonal antibody (PAb122) M00001-4 available?
Verified Customer
Verified customer
Asked: 2019-11-27
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-p53 Monoclonal antibody (PAb122) M00001-4 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-27
Question
Will anti-p53 Monoclonal antibody (PAb122) M00001-4 work for IF with epithelium of mammary gland?
Verified Customer
Verified customer
Asked: 2019-10-25
Answer
According to the expression profile of epithelium of mammary gland, TP53 is highly expressed in epithelium of mammary gland. So, it is likely that anti-p53 Monoclonal antibody (PAb122) M00001-4 will work for IF with epithelium of mammary gland.
Boster Scientific Support
Answered: 2019-10-25
Question
I have attached the WB image, lot number and protocol we used for epithelium of mammary gland using anti-p53 Monoclonal antibody (PAb122) M00001-4. Please let me know if you require anything else.
C. Roberts
Verified customer
Asked: 2019-10-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-24
Question
I would like to test anti-p53 Monoclonal antibody (PAb122) M00001-4 on human epithelium of mammary gland for research purposes, then I may be interested in using anti-p53 Monoclonal antibody (PAb122) M00001-4 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-04
Answer
The products we sell, including anti-p53 Monoclonal antibody (PAb122) M00001-4, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-04
Question
We bought anti-p53 Monoclonal antibody (PAb122) for IHC on ovarian adenocarcinoma in a previous project. I am using rat, and We want to use the antibody for IF next. I would like examining ovarian adenocarcinoma as well as blood in our next experiment. Do you have any suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2019-09-24
Answer
I have checked the website and datasheets of our anti-p53 Monoclonal antibody (PAb122) and it seems that M00001-4 has been validated on rat in both IHC and IF. Thus M00001-4 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-09-24
Question
We have seen staining in human blood. Any tips? Is anti-p53 Monoclonal antibody (PAb122) supposed to stain blood positively?
Verified Customer
Verified customer
Asked: 2019-06-20
Answer
From what I have seen in literature blood does express TP53. From what I have seen in Uniprot.org, TP53 is expressed in epithelium of mammary gland, colon, kidney, lung carcinoma, blood, ovarian adenocarcinoma, glial cell glial tumor, embryonic kidney, among other tissues. Regarding which tissues have TP53 expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 16131611
Embryonic kidney, Pubmed ID: 17525332
Glial cell, and Glial tumor, Pubmed ID: 19011621
Kidney, Pubmed ID: 15489334
Lung carcinoma, Pubmed ID: 14660794
Boster Scientific Support
Answered: 2019-06-20
Question
I was wanting to use your anti-p53 Monoclonal antibody (PAb122) for IF for human epithelium of mammary gland on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human epithelium of mammary gland identification?
Verified Customer
Verified customer
Asked: 2019-05-20
Answer
As indicated on the product datasheet, M00001-4 anti-p53 Monoclonal antibody (PAb122) has been tested for IP, IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human epithelium of mammary gland in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-20
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for epithelium of mammary gland using anti-p53 Monoclonal antibody (PAb122) M00001-4. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-02-27
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-02-27
Question
We were satisfied with the WB result of your anti-p53 Monoclonal antibody (PAb122) . However we have observed positive staining in colon cytoplasm using this antibody. Is that expected? Could you tell me where is TP53 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-07-02
Answer
According to literature, colon does express TP53. Generally TP53 expresses in cytoplasm. Regarding which tissues have TP53 expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 16131611
Embryonic kidney, Pubmed ID: 17525332
Glial cell, and Glial tumor, Pubmed ID: 19011621
Kidney, Pubmed ID: 15489334
Lung carcinoma, Pubmed ID: 14660794
Boster Scientific Support
Answered: 2018-07-02
Question
I have a question about product M00001-4, anti-p53 Monoclonal antibody (PAb122) . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-02-09
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00001-4 anti-p53 Monoclonal antibody (PAb122) , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-02-09
Question
I see that the anti-p53 Monoclonal antibody (PAb122) M00001-4 works with IF, what is the protocol used to produce the result images on the product page?
N. Evans
Verified customer
Asked: 2016-09-22
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-09-22
Question
Is a blocking peptide available for product anti-p53 Monoclonal antibody (PAb122) (M00001-4)?
G. Gonzalez
Verified customer
Asked: 2015-12-01
Answer
We do provide the blocking peptide for product anti-p53 Monoclonal antibody (PAb122) (M00001-4). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-12-01
Question
Is this M00001-4 anti-p53 Monoclonal antibody (PAb122) reactive to the isotypes of TP53?
C. Dhar
Verified customer
Asked: 2015-01-23
Answer
The immunogen of M00001-4 anti-p53 Monoclonal antibody (PAb122) is SV40 transformed 3T3 cells. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-01-23
Question
Does anti-p53 Monoclonal antibody (PAb122) M00001-4 work on zebrafish IP with ovarian adenocarcinoma?
J. Gonzalez
Verified customer
Asked: 2014-07-15
Answer
Our lab technicians have not validated anti-p53 Monoclonal antibody (PAb122) M00001-4 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-p53 Monoclonal antibody (PAb122) M00001-4 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish ovarian adenocarcinoma in IP, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-07-15
Question
We are currently using anti-p53 Monoclonal antibody (PAb122) M00001-4 for rat tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?
V. Parker
Verified customer
Asked: 2013-03-18
Answer
The anti-p53 Monoclonal antibody (PAb122) (M00001-4) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-03-18
