Product Info Summary
SKU: | RP1034 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC |
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Product info
Product Name
Anti-P Glycoprotein/ABCB1 Antibody
View all MDR1/ABCB1 Antibodies
SKU/Catalog Number
RP1034
PB0162 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-P Glycoprotein/ABCB1 Antibody catalog # RP1034. Tested in IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-P Glycoprotein/ABCB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1034)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human P Glycoprotein, different from the related rat sequence by twelve amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1034 is reactive to TP53 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
39 kDa
Calculated molecular weight
141479 MW
Background of MDR1/ABCB1
P-GP, also called ABCB1 or PGY1, is a glycoprotein that in humans is encoded by the ABCB1 gene. It is mapped to 7q21.12. P-GP is a well-characterized ABC-transporter (which transports a wide variety of substrates across extra- and intracellular membranes) of the MDR/TAP subfamily. It is an important protein of the cell membrane that pumps many foreign substances out of cells. More formally, it is an ATP-dependent drug efflux pump with broad substrate specificity. P-GP is an ATP-dependent drug efflux pump forxenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood–brain barrier.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
RP1034 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Mouse, Rat, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Positive Control
IHC: Human Lung Cancer Tissue, Mouse Kidney Tissue, Rat Kidney Tissue
IHC-F: Mouse Intestine Tissue, Rat Kidney Tissue
Validation Images & Assay Conditions
Click image to see more details
Anti-P Glycoprotein Picoband antibody, RP1034-1.JPG
IHC(F): Mouse Intestine Tissue
Click image to see more details
Anti-P Glycoprotein Picoband antibody, RP1034-2.JPG
IHC(F): Rat Kidney Tissue
Click image to see more details
Anti-P Glycoprotein Picoband antibody, RP1034-3.JPG
IHC(P): Human Lung Cancer Tissue
Click image to see more details
Anti-P Glycoprotein Picoband antibody, RP1034-4.JPG
IHC(P): Mouse Kidney Tissue
Click image to see more details
Anti-P Glycoprotein Picoband antibody, RP1034-5.JPG
IHC(P): Rat Kidney Tissue
Protein Target Info & Infographic
Gene/Protein Information For TP53 (Source: Uniprot.org, NCBI)
Gene Name
TP53
Full Name
ATP-dependent translocase ABCB1
Weight
141479 MW
Superfamily
ABC transporter superfamily
Alternative Names
Multidrug resistance protein 1;3.6.3.44;ATP-binding cassette sub-family B member 1;P-glycoprotein 1;CD243;ABCB1;MDR1, PGY1; ABCB1 ABC20, CD243, CLCS, GP170, MDR1, P-GP, PGY1, p-170 ATP binding cassette subfamily B member 1 ATP-dependent translocase ABCB1|ATP-binding cassette, sub-family B (MDR/TAP), member 1|P-glycoprotein 1|colchicin sensitivity|doxorubicin resistance|multidrug resistance protein 1|phospholipid transporter ABCB1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TP53, check out the TP53 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TP53: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-P Glycoprotein/ABCB1 Antibody (RP1034)
Hello CJ!
RP1034 has been cited in 32 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Panchamia, Shail. (2020). To Investigate The Impact Of Gut Bacteria On Efflux Transporter Expression And Function In Gastrointestinal Mucosae. Retrieved from the University of Minnesota Digital Conservancy, https://hdl.handle.net/11299/215018.
Species: Mouse
RP1034 usage in article: APP:IHC, SAMPLE:INTESTINE AND COLON TISSUE, DILUTION:1:200
The natural compound GL22, isolated from Ganoderma mushrooms, suppresses tumor growth by altering lipid metabolism and triggering cell death
Metronomic chemotherapy remodel cancer-associated fibroblasts to decrease chemoresistance of gastric cancer in nude mice
Acetazolamide Suppresses Multi-Drug Resistance-Related Protein 1 and P-Glycoprotein Expression by Inhibiting Aquaporins Expression in a Mesial Temporal Epilepsy Rat Model
Piwil2 is reactivated by HPV oncoproteins and initiates cell reprogramming via epigenetic regulation during cervical cancer tumorigenesis
P-glycoprotein Mediates Postoperative Peritoneal Adhesion Formation by Enhancing Phosphorylation of the Chloride Channel-3
Effects of human umbilical cord mesenchymal stem cell transplantation combined with minimally invasive hematoma aspiration on intracerebral hemorrhage in rats.
Lactotransferrin expression is downregulated and affects the mitogen-activated protein kinase pathway in gastric cancer
Correlation ofp53gene mutation and expression of P53 protein in cholangiocarcinoma
MDM4 Overexpressed in Acute Myeloid Leukemia Patients with Complex Karyotype and Wild-TypeTP53
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Customer Q&As
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4 Customer Q&As for Anti-P Glycoprotein/ABCB1 Antibody
Question
We are currently using anti-P Glycoprotein/ABCB1 antibody RP1034 for rat tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-22
Answer
The anti-P Glycoprotein/ABCB1 antibody (RP1034) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-22
Question
I have a question about product RP1034, anti-P Glycoprotein/ABCB1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-28
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free RP1034 anti-P Glycoprotein/ABCB1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-28
Question
I see that the anti-P Glycoprotein/ABCB1 antibody RP1034 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-12-20
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-12-20
Question
Will anti-P Glycoprotein/ABCB1 antibody RP1034 work on bovine IHC with thalamus?
R. Zhao
Verified customer
Asked: 2013-05-29
Answer
Our lab technicians have not tested anti-P Glycoprotein/ABCB1 antibody RP1034 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-P Glycoprotein/ABCB1 antibody RP1034 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine thalamus in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-05-29