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Product Info Summary
| SKU: | P00001-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, ICC, WB |
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Product info
Product Name
Anti-p53 (acetyl K370) TP53 Rabbit Monoclonal Antibody
SKU/Catalog Number
P00001-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-p53 (acetyl K370) TP53 Rabbit Monoclonal Antibody catalog # P00001-1. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-p53 (acetyl K370) TP53 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00001-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
DEO-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human p53 (acetyl K370)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P00001-1 is reactive to TP53 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
15 kDa
Calculated molecular weight
43653 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00001-1 is guaranteed for IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
IP 1:50
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Western blot analysis of p53 (acetyl K370) expression in HeLa cell lysate treated with Trichostatin A.
Protein Target Info & Infographic
Gene/Protein Information For TP53 (Source: Uniprot.org, NCBI)
Gene Name
TP53
Full Name
Cellular tumor antigen p53
Weight
43653 MW
Superfamily
p53 family
Alternative Names
Cellular tumor antigen p53;Antigen NY-CO-13;Phosphoprotein p53;Tumor suppressor p53;TP53;P53; TP53 BCC7, BMFS5, LFS1, P53, TRP53 tumor protein p53 cellular tumor p53| NY-CO-13|mutant tumor protein 53|p53 tumor suppressor|phosphoprotein p53|transformation-related protein 53|tumor protein 53|tumor supressor p53
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TP53, check out the TP53 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TP53: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-p53 (acetyl K370) TP53 Rabbit Monoclonal Antibody (P00001-1)
Hello CJ!
P00001-1 has been cited in 17 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
P-glycoprotein Mediates Postoperative Peritoneal Adhesion Formation by Enhancing Phosphorylation of the Chloride Channel-3
Effects of human umbilical cord mesenchymal stem cell transplantation combined with minimally invasive hematoma aspiration on intracerebral hemorrhage in rats.
Lactotransferrin expression is downregulated and affects the mitogen-activated protein kinase pathway in gastric cancer
Correlation ofp53gene mutation and expression of P53 protein in cholangiocarcinoma
Suspension culture combined with chemotherapeutic agents for sorting of breast cancer stem cells
A novel polysaccharide from Sargassum integerrimum induces apoptosis in A549 cells and prevents angiogensis in vitro and in vivo
TALENs-directed?knockout?of the?full-length?transcription?factor?Nrf1? that?represses?malignantbehaviour?of?human?hepatocellular?carcinoma?(HepG2)?cells
Effect of ligustilide on Ang II-induced hypertrophy in cardiomyocytes and the potential mechanisms
Feng D, Wu J, Tian Y, Zhou H, Zhou Y, Hu W, Zhao W, Wei H, Ling B, Ma C. Plos One. 2013 Nov 19;8(11):E80657. Doi: 10.1371/Journal.Pone.0080657. Ecollection 2013. Targeting Of Histone Deacetylases To Reactivate Tumour Suppressor Genes And Its Thera...
Li W, Wu D, Wei B, Wang S, Sun H, Li X, Zhang F, Zhang C, Xin Y. Afr J Tradit Complement Altern Med. 2014 Aug 23;11(5):99-104. Ecollection 2014. Anti-Tumor Effect Of Cactus Polysaccharides On Lung Squamous Carcinoma Cells (Sk-Mes-1).
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-p53 (acetyl K370) TP53 Rabbit Monoclonal Antibody
Question
We need to test anti-p53 (acetyl K370) Rabbit Monoclonal antibody P00001-1 on human ovarian adenocarcinoma for research purposes, then I may be interested in using anti-p53 (acetyl K370) Rabbit Monoclonal antibody P00001-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-25
Answer
The products we sell, including anti-p53 (acetyl K370) Rabbit Monoclonal antibody P00001-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-25
Question
We have seen staining in human epithelium of mammary gland. Are there any suggestions? Is anti-p53 (acetyl K370) Rabbit Monoclonal antibody supposed to stain epithelium of mammary gland positively?
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
According to literature epithelium of mammary gland does express TP53. According to Uniprot.org, TP53 is expressed in epithelium of mammary gland, colon, kidney, lung carcinoma, blood, ovarian adenocarcinoma, glial cell glial tumor, embryonic kidney, among other tissues. Regarding which tissues have TP53 expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 16131611
Embryonic kidney, Pubmed ID: 17525332
Glial cell, and Glial tumor, Pubmed ID: 19011621
Kidney, Pubmed ID: 15489334
Lung carcinoma, Pubmed ID: 14660794
Boster Scientific Support
Answered: 2019-11-08
Question
I was wanting to use your anti-p53 (acetyl K370) Rabbit Monoclonal antibody for WB for human ovarian adenocarcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human ovarian adenocarcinoma identification?
Verified Customer
Verified customer
Asked: 2019-09-26
Answer
You can see on the product datasheet, P00001-1 anti-p53 (acetyl K370) Rabbit Monoclonal antibody has been tested for IP, IF, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human ovarian adenocarcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-26
Question
We are currently using anti-p53 (acetyl K370) Rabbit Monoclonal antibody P00001-1 for human tissue, and we are well pleased with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-18
Answer
The anti-p53 (acetyl K370) Rabbit Monoclonal antibody (P00001-1) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-18
Question
Does P00001-1 anti-p53 (acetyl K370) Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-12
Answer
You can see on the product datasheet, P00001-1 anti-p53 (acetyl K370) Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-12
Question
Please see the WB image, lot number and protocol we used for ovarian adenocarcinoma using anti-p53 (acetyl K370) Rabbit Monoclonal antibody P00001-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-06-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-03
Question
Is this P00001-1 anti-p53 (acetyl K370) Rabbit Monoclonal antibody reactive to the isotypes of TP53?
Verified Customer
Verified customer
Asked: 2019-05-23
Answer
The immunogen of P00001-1 anti-p53 (acetyl K370) Rabbit Monoclonal antibody is A synthesized peptide derived from human p53 (acetyl K370). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-23
Question
We were well pleased with the WB result of your anti-p53 (acetyl K370) Rabbit Monoclonal antibody. However we have observed positive staining in embryonic kidney cytoplasm using this antibody. Is that expected? Could you tell me where is TP53 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-02-12
Answer
From literature, embryonic kidney does express TP53. Generally TP53 expresses in cytoplasm. Regarding which tissues have TP53 expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 16131611
Embryonic kidney, Pubmed ID: 17525332
Glial cell, and Glial tumor, Pubmed ID: 19011621
Kidney, Pubmed ID: 15489334
Lung carcinoma, Pubmed ID: 14660794
Boster Scientific Support
Answered: 2019-02-12
Question
We want using your anti-p53 (acetyl K370) Rabbit Monoclonal antibody for tp53 regulates transcription of genes involved in g1 cell cycle arrest studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-12-26
Answer
I appreciate your inquiry. This P00001-1 anti-p53 (acetyl K370) Rabbit Monoclonal antibody is tested on hela cell lysate. It is guaranteed to work for IP, IF, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-12-26
Question
Can you help my question with product P00001-1, anti-p53 (acetyl K370) Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-08-30
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free P00001-1 anti-p53 (acetyl K370) Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-08-30
Question
Is there a BSA free version of anti-p53 (acetyl K370) Rabbit Monoclonal antibody P00001-1 available?
Verified Customer
Verified customer
Asked: 2018-03-07
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-p53 (acetyl K370) Rabbit Monoclonal antibody P00001-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-03-07
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for ovarian adenocarcinoma using anti-p53 (acetyl K370) Rabbit Monoclonal antibody P00001-1. Let me know if you need anything else.
S. Williams
Verified customer
Asked: 2017-09-01
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-09-01
Question
I see that the anti-p53 (acetyl K370) Rabbit Monoclonal antibody P00001-1 works with WB, what is the protocol used to produce the result images on the product page?
D. Jha
Verified customer
Asked: 2016-03-11
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-03-11
Question
We ordered your anti-p53 (acetyl K370) Rabbit Monoclonal antibody for WB on colon in the past. I am using human, and We want to use the antibody for ICC next. We want examining colon as well as blood in our next experiment. Could you please give me some suggestion on which antibody would work the best for ICC?
F. Krishna
Verified customer
Asked: 2014-12-26
Answer
I looked at the website and datasheets of our anti-p53 (acetyl K370) Rabbit Monoclonal antibody and it seems that P00001-1 has been tested on human in both WB and ICC. Thus P00001-1 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2014-12-26
Question
Does anti-p53 (acetyl K370) Rabbit Monoclonal antibody P00001-1 work for WB with ovarian adenocarcinoma?
A. Jones
Verified customer
Asked: 2014-07-04
Answer
According to the expression profile of ovarian adenocarcinoma, TP53 is highly expressed in ovarian adenocarcinoma. So, it is likely that anti-p53 (acetyl K370) Rabbit Monoclonal antibody P00001-1 will work for WB with ovarian adenocarcinoma.
Boster Scientific Support
Answered: 2014-07-04
Question
Is a blocking peptide available for product anti-p53 (acetyl K370) Rabbit Monoclonal antibody (P00001-1)?
G. Anderson
Verified customer
Asked: 2013-10-07
Answer
We do provide the blocking peptide for product anti-p53 (acetyl K370) Rabbit Monoclonal antibody (P00001-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-10-07
