Click image to see more details
Product Info Summary
| SKU: | P00001-7 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Phospho-p53 (S376) TP53 Rabbit Monoclonal Antibody
SKU/Catalog Number
P00001-7
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-p53 (S376) TP53 Rabbit Monoclonal Antibody catalog # P00001-7. Tested in WB application. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-p53 (S376) TP53 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00001-7)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
DGA-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-p53 (S376)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P00001-7 is reactive to TP53 in Human
Reconstitution
Observed Molecular Weight
53 kDa
Calculated molecular weight
43653 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00001-7 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
Validation Images & Assay Conditions
Click image to see more details
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Click image to see more details
Western blot analysis of Phospho-p53 (S376) in HEK293 cell lysates treated with Calyculin A and Okadaic Acid.
Protein Target Info & Infographic
Gene/Protein Information For TP53 (Source: Uniprot.org, NCBI)
Gene Name
TP53
Full Name
Cellular tumor antigen p53
Weight
43653 MW
Superfamily
p53 family
Alternative Names
Cellular tumor antigen p53;Antigen NY-CO-13;Phosphoprotein p53;Tumor suppressor p53;TP53;P53; TP53 BCC7, BMFS5, LFS1, P53, TRP53 tumor protein p53 cellular tumor p53| NY-CO-13|mutant tumor protein 53|p53 tumor suppressor|phosphoprotein p53|transformation-related protein 53|tumor protein 53|tumor supressor p53
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TP53, check out the TP53 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TP53: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-p53 (S376) TP53 Rabbit Monoclonal Antibody (P00001-7)
Hello CJ!
No publications found for P00001-7
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Phospho-p53 (S376) TP53 Rabbit Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Phospho-p53 (S376) TP53 Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
3 Customer Q&As for Anti-Phospho-p53 (S376) TP53 Rabbit Monoclonal Antibody
Question
My colleagues were satisfied with the WB result of your anti-Phospho-p53 (S376) Rabbit Monoclonal antibody. However we have seen positive staining in lung carcinoma cytoplasm using this antibody. Is that expected? Could you tell me where is TP53 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-04-29
Answer
According to literature, lung carcinoma does express TP53. Generally TP53 expresses in cytoplasm. Regarding which tissues have TP53 expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 16131611
Embryonic kidney, Pubmed ID: 17525332
Glial cell, and Glial tumor, Pubmed ID: 19011621
Kidney, Pubmed ID: 15489334
Lung carcinoma, Pubmed ID: 14660794
Boster Scientific Support
Answered: 2020-04-29
Question
We have been able to see staining in human blood. Are there any suggestions? Is anti-Phospho-p53 (S376) Rabbit Monoclonal antibody supposed to stain blood positively?
Verified Customer
Verified customer
Asked: 2019-06-17
Answer
From what I have seen in literature blood does express TP53. From what I have seen in Uniprot.org, TP53 is expressed in epithelium of mammary gland, colon, kidney, lung carcinoma, blood, ovarian adenocarcinoma, glial cell glial tumor, embryonic kidney, among other tissues. Regarding which tissues have TP53 expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 16131611
Embryonic kidney, Pubmed ID: 17525332
Glial cell, and Glial tumor, Pubmed ID: 19011621
Kidney, Pubmed ID: 15489334
Lung carcinoma, Pubmed ID: 14660794
Boster Scientific Support
Answered: 2019-06-17
Question
We are currently using anti-Phospho-p53 (S376) Rabbit Monoclonal antibody P00001-7 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2017-11-13
Answer
The anti-Phospho-p53 (S376) Rabbit Monoclonal antibody (P00001-7) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-11-13
