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Product Info Summary
| SKU: | A06345-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-OPA3 Antibody Picoband®
SKU/Catalog Number
A06345-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-OPA3 Antibody Picoband® catalog # A06345-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-OPA3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06345-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human OPA3 recombinant protein (Position: M1-K179).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A06345-1 is reactive to OPA3 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
20 kDa
Calculated molecular weight
39162 MW
Background of OPA3
Optic atrophy 3 protein is a protein that in humans is encoded by the OPA3 gene. The mouse ortholog of this protein co-purifies with the mitochondrial inner membrane. Mutations in this gene have been shown to result in 3-methylglutaconic aciduria type III and autosomal dominant optic atrophy and cataract. Multiple transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06345-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human 293T whole cell, human A549 whole cell
FCM: U937 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of OPA3 using anti-OPA3 antibody (A06345-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human A549 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OPA3 antigen affinity purified polyclonal antibody (Catalog # A06345-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OPA3 at approximately 20 kDa. The expected band size for OPA3 is at 20 kDa.
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Figure 2. Flow Cytometry analysis of U937 cells using anti-OPA3 antibody (A06345-1).
Overlay histogram showing U937 cells stained with A06345-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OPA3 Antibody (A06345-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For OPA3 (Source: Uniprot.org, NCBI)
Gene Name
OPA3
Full Name
Optic atrophy 3 protein
Weight
39162 MW
Superfamily
OPA3 family
Alternative Names
Spindle and kinetochore-associated protein 2; Protein FAM33A; SKA2; FAM33A OPA3 MGA3 outer mitochondrial membrane lipid metabolism regulator OPA3 optic atrophy 3 protein|OPA3 outer mitochondrial membrane lipid metabolism regulator|Optic atrophy 3 (Iraqi-Jewish optic atrophy plus)|optic atrophy 3 (autosomal recessive, with chorea and spastic paraplegia)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on OPA3, check out the OPA3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for OPA3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-OPA3 Antibody Picoband® (A06345-1)
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