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Product Info Summary
| SKU: | A06734 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-SKA1 Antibody
SKU/Catalog Number
A06734
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-SKA1 Antibody (Catalog # A06734). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
SKA1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-SKA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06734)
Host
Rabbit
Contents
SKA1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
SKA1 antibody was raised against a 13 amino acid synthetic peptide from near the carboxy terminus of human SKA1. The immunogen is located within amino acids 180 - 230 of SKA1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
VTI1a antibody is human, mouse and rat reactive. At least two isoforms of VTI1a are known to exist; this antibody will detect both isoforms. VTI1a antibody is predicted to not cross-react with VTI1b.
Reactive Species
A06734 is reactive to SKA1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
29484 MW
Background of Ska1
Upon entry into mitosis, the cell's microtubule (MT) network forms the mitotic spindle, allowing the segregation of paired chromosomes. Proteinaceous structures on centromeric chromatin termed kinetochores (KT) are essential for the proper attachment of the chromosomes to the spindle MTs. A recently discovered spindle and kinetochore complex, comprised of proteins SKA1, SKA2, and SKA3, has been found to be required for stable KT-MT interactions and timely anaphase onset. Depletion of either SKA1 or SKA2 by siRNA results in the loss of both proteins from the KT, but does not impact overall KT structure. Cells depleted of the SKA complex undergo a prolonged checkpoint-dependent delay in a metaphase-like state, indicating the importance of the SKA complex in the maintenance of the metaphase plate and spindle checkpoint silencing.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06734 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
SKA1 antibody can be used for detection of SKA1 by Western blot at 0.5 - 1 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of SKA1 in A549 cell lysate with SKA1 antibody at (A) 0.5 and (B) 1 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For SKA1 (Source: Uniprot.org, NCBI)
Gene Name
SKA1
Full Name
Spindle and kinetochore-associated protein 1
Weight
29484 MW
Superfamily
SKA1 family
Alternative Names
C18orf24, C18orf24, Spindle and kinetochore-associated protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SKA1, check out the SKA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SKA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SKA1 Antibody (A06734)
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No publications found for A06734
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