Anti-SKA1 Antibody

Product Name

Anti-SKA1 Antibody

View all Ska1 Antibodies

SKU/Catalog Number

A06734

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-SKA1 Antibody (Catalog # A06734). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

SKA1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-SKA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06734)

Host

Rabbit

Contents

SKA1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

Clone: SK7

Isotype

IgG

Immunogen

SKA1 antibody was raised against a 13 amino acid synthetic peptide from near the carboxy terminus of human SKA1. The immunogen is located within amino acids 180 - 230 of SKA1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

VTI1a antibody is human, mouse and rat reactive. At least two isoforms of VTI1a are known to exist; this antibody will detect both isoforms. VTI1a antibody is predicted to not cross-react with VTI1b.

Reactive Species

A06734 is reactive to Ska1 in Human, Mouse, Rat

Applications

A06734 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

29484 MW

Background of Ska1

Upon entry into mitosis, the cell's microtubule (MT) network forms the mitotic spindle, allowing the segregation of paired chromosomes. Proteinaceous structures on centromeric chromatin termed kinetochores (KT) are essential for the proper attachment of the chromosomes to the spindle MTs. A recently discovered spindle and kinetochore complex, comprised of proteins SKA1, SKA2, and SKA3, has been found to be required for stable KT-MT interactions and timely anaphase onset. Depletion of either SKA1 or SKA2 by siRNA results in the loss of both proteins from the KT, but does not impact overall KT structure. Cells depleted of the SKA complex undergo a prolonged checkpoint-dependent delay in a metaphase-like state, indicating the importance of the SKA complex in the maintenance of the metaphase plate and spindle checkpoint silencing.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

SKA1 antibody can be used for detection of SKA1 by Western blot at 0.5 - 1 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of SKA1 in A549 cell lysate with SKA1 antibody at (A) 0.5 and (B) 1 μg/mL.

Gene/Protein Information For Ska1 (Source: Uniprot.org, NCBI)

Alternative Names

C18orf24; chromosome 18 open reading frame 24; MGC10200; spindle and kinetochore associated complex subunit 1; spindle and kinetochore-associated protein 1; spindle and KT (kinetochore) associated 1

For more info on Ska1, check out the Ska1 Infographic

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In this infographic, you will see the following information for Ska1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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