Anti-Phospho-Olig2 (Ser10,13,14) Antibody

Product Name

Anti-Phospho-Olig2 (Ser10,13,14) Antibody

View all OLIG2 Antibodies

SKU/Catalog Number

P02247

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Phospho-Olig2 (Ser10, 13, 14) Antibody (Catalog # P02247). Tested in WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

Cite This Product

Anti-Phospho-Olig2 (Ser10,13,14) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P02247)

Host

Rabbit

Contents

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser10,13,14 of human Olig2, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Human.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

P02247 is reactive to OLIG2 in Mouse, Rat

Reconstitution

Observed Molecular Weight

52 kDa

Calculated molecular weight

32407 MW

Background of OLIG2

Olig2 is a well conserved bHLH transcription factor that shows both anti-neural functions and pro-neural functions at different stages in the formation of the oligodendrocyte lineage (Sun et al., 2011). Olig2 is expressed in 100% of the human diffuse gliomas irrespective of grade and required for intracranial tumor formation in a genetically relevant model of malignant glioma (Ligon et al., 2004; Ligon et al., 2007). A developmentally regulated triple serine motif at positions 10, 13 and 14 in the amino terminus is well conserved across species ranging from humans to zebrafish and is essential for Olig2 proliferative function in both normal and malignant neural progenitors (Sun et al., 2011). All three serine residues must be mutated to achieve a strong loss-of-function or gain-of-function phenotype, suggesting that the phosphorylation state of Olig2 represents a significant conformational change in the amino terminus (Sun et al., 2011).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

P02247 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000
IHC: 1:1000

Validation Images & Assay Conditions

Gene/Protein Information For OLIG2 (Source: Uniprot.org, NCBI)

Alternative Names

Basic domain helix loop helix protein class B 6 antibody, Basic domain helix loop helix protein class B6 antibody, BHLH B6 antibody, BHLHB 6 antibody, bHLHb6 antibody, bHLHe21 antibody, Class B basic helix-loop-helix protein 6 antibody, Class E basic helix-loop-helix protein 21 antibody, Olig 1 antibody, Olig1 antibody, OLIG1_HUMAN antibody, Oligo 1 antibody, Oligo1 antibody, Oligodendrocyte lineage transcription factor 1 antibody, Oligodendrocyte specific bHLH transcription factor 1 antibody, Oligodendrocyte transcription factor 1 antibody OLIG2 BHLHB1, OLIGO2, PRKCBP2, RACK17, bHLHe19 oligodendrocyte transcription factor 2 oligodendrocyte transcription factor 2|basic domain, helix-loop-helix protein, class B, 1|class B basic helix-loop-helix protein 1|class E basic helix-loop-helix protein 19|human protein kinase C-binding protein RACK17|oligodendrocyte lineage transcription factor 2|oligodendrocyte-specific bHLH transcription factor 2|protein kinase C-binding protein 2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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