Anti-CNTF Antibody Picoband™

Product Name

Anti-CNTF Antibody Picoband™

View all CNTF Antibodies

SKU/Catalog Number

PB9067

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CNTF Antibody Picoband™ catalog # PB9067. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CNTF Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9067)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived rat CNTF recombinant protein (Position: A2-M200). Rat CNTF shares 84% and 95% amino acid (aa) sequences identity with human and mouse CNTF, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9067 is reactive to CNTF in Mouse, Rat

Applications

PB9067 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

23 kDa

Calculated molecular weight

22854 MW

Background of CNTF

Ciliary neurotrophic factor is a protein that in humans is encoded by the CNTF gene. The protein encoded by this gene is a polypeptide hormone andnerve growth factor whose actions have mainly been studied in the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neural populations includingastrocytes. It is mapped to 11q12.1. In addition to neurotrophic effects on parasympathetic neurons, CNTF was shown to have activities on sympathetic and sensory neurons. CNTF appears not to be involved in motor neuron survival during development. human CNTF has a trophic influence on degenerating striatal neurons as well as on critical nonstriatal regions. CNTF plays a role in a cardiac signal transduction pathway that regulates obesity-related left ventricular hypertrophy.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA , 0.1-0.5μg/ml, Rat, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Rat

Validation Images & Assay Conditions

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Figure 1. IHC analysis of CNTF using anti-CNTF antibody (PB9067).
CNTF was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CNTF Antibody (PB9067) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

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Figure 2. IHC analysis of CNTF using anti-CNTF antibody (PB9067).
CNTF was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CNTF Antibody (PB9067) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

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Figure 3. Western blot analysis of CNTF using anti-CNTF antibody (PB9067).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat PC-12 whole cell lysates,
Lane 2: rat NRK whole cell lysates,
Lane 3: rat RH-35 whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat spleen tissue lysates,
Lane 7: rat testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CNTF antigen affinity purified polyclonal antibody (Catalog # PB9067) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CNTF at approximately 23 kDa. The expected band size for CNTF is at 23 kDa.

Gene/Protein Information For CNTF (Source: Uniprot.org, NCBI)

Alternative Names

ciliary neurotrophic factor; CNTF; HCNTF CNTF HCNTF ciliary neurotrophic factor ciliary neurotrophic factor|Ciliary Neuronotrophic Factor

For more info on CNTF, check out the CNTF Infographic

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In this infographic, you will see the following information for CNTF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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