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Product Info Summary
| SKU: | M02247 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Olig2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M02247
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Olig2 Rabbit Monoclonal Antibody catalog # M02247. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Olig2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02247)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ADC-15
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Olig2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02247 is reactive to OLIG2 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
39 kDa
Calculated molecular weight
32385 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02247 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
WB: Human oligodendroglioma
IHC: human mouse brain tissue
Validation Images & Assay Conditions
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Western blot analysis of Olig2 in Human oligodendroglioma lysate.
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Immunohistochemical analysis of paraffin-embedded human mouse brain, using Olig2 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For OLIG2 (Source: Uniprot.org, NCBI)
Gene Name
OLIG2
Full Name
Oligodendrocyte transcription factor 2
Weight
32385 MW
Alternative Names
Oligodendrocyte transcription factor 2;Oligo2;Class B basic helix-loop-helix protein 1;bHLHb1;Class E basic helix-loop-helix protein 19;bHLHe19;Protein kinase C-binding protein 2;Protein kinase C-binding protein RACK17;OLIG2;BHLHB1, BHLHE19, PRKCBP2, RACK17; OLIG2 BHLHB1, OLIGO2, PRKCBP2, RACK17, bHLHe19 oligodendrocyte transcription factor 2 oligodendrocyte transcription factor 2|basic domain, helix-loop-helix protein, class B, 1|class B basic helix-loop-helix protein 1|class E basic helix-loop-helix protein 19|human protein kinase C-binding protein RACK17|oligodendrocyte lineage transcription factor 2|oligodendrocyte-specific bHLH transcription factor 2|protein kinase C-binding protein 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on OLIG2, check out the OLIG2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for OLIG2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Olig2 Rabbit Monoclonal Antibody (M02247)
Hello CJ!
M02247 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
[Met5]-enkephalin preserves diffusion metrics in EAE mice
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Customer Q&As
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4 Customer Q&As for Anti-Olig2 Rabbit Monoclonal Antibody
Question
Does M02247 anti-Olig2 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
You can see on the product datasheet, M02247 anti-Olig2 Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-10
Question
Do you have a BSA free version of anti-Olig2 Rabbit Monoclonal antibody M02247 available?
Verified Customer
Verified customer
Asked: 2019-06-06
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Olig2 Rabbit Monoclonal antibody M02247 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-06
Question
I see that the anti-Olig2 Rabbit Monoclonal antibody M02247 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-03-21
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-03-21
Question
Will anti-Olig2 Rabbit Monoclonal antibody M02247 work on canine WB with globus pallidus?
Verified Customer
Verified customer
Asked: 2017-10-04
Answer
Our lab technicians have not tested anti-Olig2 Rabbit Monoclonal antibody M02247 on canine. You can run a BLAST between canine and the immunogen sequence of anti-Olig2 Rabbit Monoclonal antibody M02247 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine globus pallidus in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-10-04
