Anti-OLIG2 Antibody

Product Name

Anti-OLIG2 Antibody

View all OLIG2 Antibodies

SKU/Catalog Number

A02247-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-OLIG2 Antibody (Catalog # A02247-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

OLIG2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-OLIG2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02247-1)

Host

Rabbit

Contents

OLIG2 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

OLIG2 antibody was raised against a 15 amino acid peptide near the amino terminus of human OLIG2. The immunogen is located within amino acids 80 - 130 of OLIG2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

OLIG2 antibody is human, mouse and rat reactive. It is predicted to not cross-react with other members of the OLIG family of proteins. At least three isoforms of OLIG2 are known to exist; this antibody will detect all three isoforms.

Reactive Species

A02247-1 is reactive to OLIG2 in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

32385 MW

Background of OLIG2

The oligodendrocyte transcription factors 1 and 2 (OLIG1 and OLIG2, respectively) make up part of basic helix-loop-helix (bHLH) family of transcription factors that are specifically expressed in zones of the neuroepithelium from which oligodendrocyte precursors emerge (1). Both OLIG1 and OLIG2 genes are downstream targets of Sonic hedgehog and are expressed exclusively in the central nervous system (2). OLIG2 is first observed in the ventral most p3 progenitor domain of the ventral neural tube while OLIG1 is first expressed in the dorsal portion of the p3 domain (2). Mice overexpressing OLIG2 exhibit impaired potassium channel expression in neural progenitors and proliferation of these cells similar to that seen in Down Syndrome, suggesting that OLIG2 may play a role in this pathology (3).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A02247-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

OLIG2 antibody can be used for detection of OLIG2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For OLIG2 (Source: Uniprot.org, NCBI)

Alternative Names

BHLHB1, OLIGO2, RACK17, PRKCBP2, bHLHe19, BHLHB1, BHLHE19, Oligodendrocyte transcription factor 2, Class B basic helix-loop-helix protein 1, Oligo2 OLIG2 BHLHB1, OLIGO2, PRKCBP2, RACK17, bHLHe19 oligodendrocyte transcription factor 2 oligodendrocyte transcription factor 2|basic domain, helix-loop-helix protein, class B, 1|class B basic helix-loop-helix protein 1|class E basic helix-loop-helix protein 19|human protein kinase C-binding protein RACK17|oligodendrocyte lineage transcription factor 2|oligodendrocyte-specific bHLH transcription factor 2|protein kinase C-binding protein 2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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