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Product Info Summary
| SKU: | PB9851 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-CNTF Antibody Picoband®
SKU/Catalog Number
PB9851
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CNTF Antibody Picoband® catalog # PB9851. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CNTF Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9851)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human CNTF recombinant protein (Position: H12-M200). Human CNTF shares 84.1% and 85.2% amino acid (aa) sequence identity with mouse and rat CNTF, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9851 is reactive to CNTF in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
27 kDa
Calculated molecular weight
22931 MW
Background of CNTF
Ciliary neurotrophic factor (CNTF) is a potent polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes survival, neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. This gene is located on chromosome 11, as determined using human-hamster somatic cell hybrids. The CNTF protein is highly conserved in evolution. The amino acid (aa) sequences of rat and rabbit CNTF translated from cDNAs display approx. 85% homology with the deduced aa sequence encoding human CNTF. CNTF induces weight loss and improves glucose tolerance in humans and rodents. It is thought to act centrally by inducing hypothalamic neurogenesis to modulate food intake and peripherally by altering hepatic gene expression, in a manner similar to that of leptin.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9851 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA, 0.1-0.5μg/ml, -
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: MCF-7 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CNTF using anti-CNTF antibody (PB9851).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CNTF antigen affinity purified polyclonal antibody (Catalog # PB9851) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CNTF at approximately 27 kDa. The expected band size for CNTF is at 23 kDa.
Protein Target Info & Infographic
Gene/Protein Information For CNTF (Source: Uniprot.org, NCBI)
Gene Name
CNTF
Full Name
Ciliary neurotrophic factor
Weight
22931 MW
Superfamily
CNTF family
Alternative Names
Ciliary neurotrophic factor;CNTF;CNTF; CNTF HCNTF ciliary neurotrophic factor ciliary neurotrophic factor|Ciliary Neuronotrophic Factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CNTF, check out the CNTF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CNTF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CNTF Antibody Picoband® (PB9851)
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Customer Q&As
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6 Customer Q&As for Anti-CNTF Antibody Picoband®
Question
I have attached the WB image, lot number and protocol we used for brain using anti-CNTF antibody PB9851. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-01-29
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-29
Question
I was wanting to use your anti-CNTF antibody for WB for human brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human brain identification?
A. Jha
Verified customer
Asked: 2019-05-17
Answer
You can see on the product datasheet, PB9851 anti-CNTF antibody has been validated for ELISA, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-17
Question
Does anti-CNTF antibody PB9851 work for WB with brain?
Verified Customer
Verified customer
Asked: 2017-12-25
Answer
According to the expression profile of brain, CNTF is highly expressed in brain. So, it is likely that anti-CNTF antibody PB9851 will work for WB with brain.
Boster Scientific Support
Answered: 2017-12-25
Question
We are currently using anti-CNTF antibody PB9851 for human tissue, and we are satisfied with the ELISA results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2017-08-17
Answer
The anti-CNTF antibody (PB9851) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-08-17
Question
I see that the anti-CNTF antibody PB9851 works with WB, what is the protocol used to produce the result images on the product page?
S. Zhang
Verified customer
Asked: 2015-11-05
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-11-05
Question
Is a blocking peptide available for product anti-CNTF antibody (PB9851)?
R. Zhang
Verified customer
Asked: 2014-10-09
Answer
We do provide the blocking peptide for product anti-CNTF antibody (PB9851). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-10-09
