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Product Info Summary
| SKU: | PA1829 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC, IHC-F, ICC, WB |
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Product info
Product Name
Anti-NM23A/NME1 Antibody Picoband®
SKU/Catalog Number
PA1829
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NM23A/NME1 Antibody catalog # PA1829. Tested in Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NM23A/NME1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1829)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human NM23A, different from the related mouse sequence by two amino acids and from rat sequence by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1829 is reactive to NME1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
17 kDa
Calculated molecular weight
17 kDa
Background of NME1
NME1 (NME/NM23 nucleoside diphosphate kinase 1) also called non-metastatic cells 1, protein (NM23A) expressed in,NM23, NM23-H1, NDPKA, GAAD or AWD, is an enzyme that in humans is encoded by the NME1 gene. The promoters of the mouse and human NME1 genes, like those of other NME genes, contain several binding sites for AP2, NF1, Sp1, LEF1, and response elements to glucocorticoid receptors. The NME1 gene is mapped on 17q21.33. Immunofluorescence microscopy demonstrated colocalization of NME1 in nuclei of B cells expressing EBNA3C. Expression of EBNA3C reversed the ability of NME1 to inhibit migration of BL and breast carcinoma cells. NM23H1 bound SET and was released from inhibition by GZMA cleavage of SET. After GZMA loading or cytotoxic T lymphocyte attack, SET and NM23H1 translocated to the nucleus and SET was degraded, allowing NM23H1 to nick chromosomal DNA. Using a Drosophila model system, Dammai et al. (2003) showed that the Drosophila NME1 homolog, awd, regulates trachea cell motility by modulating FGFR levels through a dynamin -mediated pathway.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1829 is guaranteed for Flow Cytometry, IHC, IHC-F, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human
Immunocytochemistry, 0.5-1μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: human A549 whole cell, human MCF-7 whole cell, human Hela whole cell, human PC-3 whole cell, rat brain tissue,
rat kidney tissue, mouse brain tissue, mouse kidney tissue
IHC: Rat Cerebellum tissue, human mammary cancer tissue
ICC: HELA Cell
FCM: Hela cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of NM23A using anti-NM23A antibody (PA1829).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human PC-3 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat kidney tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NM23A antigen affinity purified polyclonal antibody (Catalog # PA1829) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NM23A at approximately 17 kDa. The expected band size for NM23A is at 17 kDa.
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Figure 2. IHC analysis of NM23A using anti-NM23A antibody (PA1829).
NM23A was detected in paraffin-embedded section of Rat Cerebellum tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NM23A Antibody (PA1829) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis of NM23A using anti-NM23A antibody (PA1829).
NM23A was detected in immunocytochemical section of HELA Cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-NM23A Antibody (PA1829) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 4. Flow Cytometry analysis of Hela cells using anti-NME1 antibody (PA1829).
Overlay histogram showing Hela cells stained with PA1829 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NME1 Antibody (PA1829,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Figure 5. IHC analysis of NM23A using anti-NM23A antibody (PA1829).
NM23A was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NM23A Antibody (PA1829) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For NME1 (Source: Uniprot.org, NCBI)
Gene Name
NME1
Full Name
Nucleoside diphosphate kinase A
Weight
17 kDa
Superfamily
NDK family
Alternative Names
Nucleoside diphosphate kinase A;NDK A;NDP kinase A;2.7.4.6;Granzyme A-activated DNase;GAAD;Metastasis inhibition factor nm23;NM23-H1;Tumor metastatic process-associated protein;NME1;NDPKA, NM23; NME1 AWD, GAAD, NB, NBS, NDKA, NDPK-A, NDPKA, NM23, NM23-H1 NME/NM23 nucleoside diphosphate kinase 1 nucleoside diphosphate kinase A|NDP kinase A|epididymis secretory sperm binding protein|granzyme A-activated DNase|metastasis inhibition factor nm23|non-metastatic cells 1, protein (NM23A) expressed in|tumor metastatic process-associated protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NME1, check out the NME1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NME1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NM23A/NME1 Antibody Picoband® (PA1829)
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Customer Q&As
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5 Customer Q&As for Anti-NM23A/NME1 Antibody Picoband®
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for left adrenal gland using anti-NM23A/NME1 antibody PA1829. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-23
Question
Will anti-NM23A/NME1 antibody PA1829 work on canine Flow Cytometry with neuroblastoma?
Verified Customer
Verified customer
Asked: 2020-04-16
Answer
Our lab technicians have not validated anti-NM23A/NME1 antibody PA1829 on canine. You can run a BLAST between canine and the immunogen sequence of anti-NM23A/NME1 antibody PA1829 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine neuroblastoma in Flow Cytometry, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-16
Question
I see that the anti-NM23A/NME1 antibody PA1829 works with ICC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-09-05
Answer
You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-09-05
Question
Does anti-NM23A/NME1 antibody PA1829 work for ICC with left adrenal gland?
Verified Customer
Verified customer
Asked: 2018-07-20
Answer
According to the expression profile of left adrenal gland, NME1 is highly expressed in left adrenal gland. So, it is likely that anti-NM23A/NME1 antibody PA1829 will work for ICC with left adrenal gland.
Boster Scientific Support
Answered: 2018-07-20
Question
I was wanting to use your anti-NM23A/NME1 antibody for ICC for human left adrenal gland on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human left adrenal gland identification?
S. Johnson
Verified customer
Asked: 2015-10-28
Answer
As indicated on the product datasheet, PA1829 anti-NM23A/NME1 antibody has been validated for Flow Cytometry, IHC-P, IHC-F, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human left adrenal gland in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-10-28
