Product Info Summary
SKU: | RP1090 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-NM23A/NME1 Antibody
SKU/Catalog Number
RP1090
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NM23A/NME1 Antibody catalog # RP1090. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NM23A/NME1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1090)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human NM23A, different from the related mouse and rat sequences by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1090 is reactive to NME1 in Human, Mouse, Rat
Applications
RP1090 is guaranteed for Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
17 kDa
Calculated molecular weight
17149 MW
Background of NME1
NME1 (NME/NM23 nucleoside diphosphate kinase 1), also called non-metastatic cells 1, protein (NM23A) expressed in, NM23, NM23-H1, NDPKA, GAAD or AWD, is an enzyme that in humans is encoded by the NME1 gene. The promoters of the mouse and human NME1 genes, like those of other NME genes, contain several binding sites for AP2, NF1, Sp1, LEF1, and response elements to glucocorticoid receptors. The NME1 gene is mapped on 17q21.33. Immunofluorescence microscopy demonstrated colocalization of NME1 in nuclei of B cells expressing EBNA3C. Expression of EBNA3C reversed the ability of NME1 to inhibit migration of BL and breast carcinoma cells. NM23H1 bound SET and was released from inhibition by GZMA cleavage of SET. After GZMA loading or cytotoxic T lymphocyte attack, SET and NM23H1 translocated to the nucleus and SET was degraded, allowing NM23H1 to nick chromosomal DNA. Using a Drosophila model system, Dammai et al. (2003) showed that the Drosophila NME1 homolog, awd, regulates trachea cell motility by modulating FGFR levels through a dynamin -mediated pathway.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Flow Cytometry(Fixed), 1-3μg/1x106 cells, Human
Validation Images & Assay Conditions
![rp1090 nm23a primary antibodies wb testing 1 rp1090 nm23a primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/r/p/rp1090-nm23a-primary-antibodies-wb-testing-1.jpg)
Click image to see more details
Figure 1. Western blot analysis of NM23A/NME1 using anti-NM23A/NME1 antibody (RP1090).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: human MCF-7 whole cell lysates,
Lane 6: human A375 whole cell lysates,
Lane 7: human MOLT-4 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NM23A/NME1 antigen affinity purified polyclonal antibody (Catalog # RP1090) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NM23A/NME1 at approximately 17 kDa. The expected band size for NM23A/NME1 is at 17 kDa.
![rp1090 nm23a primary antibodies wb testing 2 rp1090 nm23a primary antibodies wb testing 2](https://www.bosterbio.com/media/catalog/product/r/p/rp1090-nm23a-primary-antibodies-wb-testing-2.jpg)
Click image to see more details
Figure 2. Western blot analysis of NM23A/NME1 using anti-NM23A/NME1 antibody (RP1090).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat liver tissue lysates,
Lane 3: rat lung tissue lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse liver tissue lysates,
Lane 7: mouse lung tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NM23A/NME1 antigen affinity purified polyclonal antibody (Catalog # RP1090) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NM23A/NME1 at approximately 17 kDa. The expected band size for NM23A/NME1 is at 17 kDa.
![rp1090 nm23a primary antibodies fcm testing 3 rp1090 nm23a primary antibodies fcm testing 3](https://www.bosterbio.com/media/catalog/product/r/p/rp1090-nm23a-primary-antibodies-fcm-testing-3.jpg)
Click image to see more details
Figure 3. Flow Cytometry analysis of HEL cells using anti-NM23A/NME1 antibody (RP1090).
Overlay histogram showing HEL cells stained with RP1090 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NM23A/NME1 Antibody (RP1090, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For NME1 (Source: Uniprot.org, NCBI)
Gene Name
NME1
Full Name
Nucleoside diphosphate kinase A
Weight
17149 MW
Superfamily
NDK family
Alternative Names
EC 2.7.4.6; GAAD; Granzyme A-activated DNase; Metastasis inhibition factor nm23; NB; NBS; NDK A; NDKA; NDP kinase A; NDPKA; NDPK-A; NM23A; NM23AWD; NM23H1; NM23-H1; NME1; non-metastatic cells 1, protein (NM23A) expressed in; nucleoside diphosphate kinase A; Tumor metastatic process-associated protein NME1 AWD, GAAD, NB, NBS, NDKA, NDPK-A, NDPKA, NM23, NM23-H1 NME/NM23 nucleoside diphosphate kinase 1 nucleoside diphosphate kinase A|NDP kinase A|epididymis secretory sperm binding protein|granzyme A-activated DNase|metastasis inhibition factor nm23|non-metastatic cells 1, protein (NM23A) expressed in|tumor metastatic process-associated protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NME1, check out the NME1 Infographic
![NME1 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NME1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NM23A/NME1 Antibody (RP1090)
Hello CJ!
RP1090 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Mutation of the nm23-H1 gene has a non-dominant role in colorectal adenocarcinoma
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Customer Q&As
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1 Customer Q&As for Anti-NM23A/NME1 Antibody
Question
We are currently using anti-NM23A/NME1 antibody RP1090 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?
S. Taylor
Verified customer
Asked: 2018-11-21
Answer
The anti-NM23A/NME1 antibody (RP1090) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-11-21