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Product Info Summary
| SKU: | EK1772 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate ). |
Product info
Product Name
Mouse Contactin-1 ELISA Kit PicoKine®
View all Contactin-1 ELISA kits
SKU/Catalog Number
EK1772
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse Contactin-1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Cntn1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate ). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse Contactin-1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1772)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: D21-S1001
Sensitivity
<50 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1772 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1772 is reactive to Cntn1 in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate ).
Application Guarantee
EK1772 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Contactin-1
Contactin 1, also known as CNTN1, is a protein which in humans is encoded by the CNTN1 gene. This gene is mapped to 15 E3; 15 46.39 cM. The protein encoded by this gene is a member of the immunoglobulin superfamily. It is a glycosylphosphatidylinositol (GPI)-anchored neuronal membrane protein that functions as a cell adhesion molecule. It may play a role in the formation of axon connections in the developing nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1772-CAP | Anti-Mouse Cntn1 Pre-coated 96-well strip microplate | 1 |
| EK1772-ST | Mouse Cntn1 Standard | 2 vials, 10 ng/tube |
| EK1772-DA | Mouse Cntn1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.007 | 0.225 | 0.336 | 0.696 | 1.119 | 1.911 | 2.220 | 2.395 |
Data Example Images
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Mouse Contactin-1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Contactin-1, Dilution ratio of 1:10, concentration in serum and plasma is 20-30 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse Contactin-1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 298 | 1189 | 4684 | 303 | 1194 | 4854 |
| Standard deviation | 12.22 | 61.83 | 257.62 | 19.09 | 88.34 | 378.6 |
| CV (%) | 4.1% | 5.2% | 5.5% | 6.3% | 7.4% | 7.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Contactin-1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 298 | 288 | 323 | 291 | 300 | 13.76 | 4.5% |
| Sample 2 | 1189 | 1277 | 1139 | 1303 | 1227 | 66.07 | 5.3% |
| Sample 3 | 4684 | 4230 | 4964 | 4885 | 4690 | 284.92 | 6.0% |
Protein Target Info & Infographic
Gene/Protein Information For Cntn1 (Source: Uniprot.Org, NCBI)
Gene Name
Cntn1
Full Name
Contactin-1
Weight
113.32kDa
Superfamily
immunoglobulin superfamily
Alternative Names
Contactin-1; Neural cell surface protein F3; CNTN1 CNTN1 F3, GP135, MYPCN contactin 1 contactin-1|glycoprotein gP135|neural cell surface protein F3
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Cntn1, check out the Cntn1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Cntn1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse Contactin-1 ELISA Kit PicoKine® (EK1772)
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Customer Q&As
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5 Customer Q&As for Mouse Contactin-1 ELISA Kit PicoKine®
Question
Q: how can I thaw whole blood sample for Contactin-1 ELISA after freezing?
L. Gandhi
Verified customer
Asked: 2020-11-30
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Contactin-1 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2020-11-30
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-09-11
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2019-05-17
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-05-17
Question
Q: if the enzyme conjugated Contactin-1 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Contactin-1 antigen?
Z. Krishna
Verified customer
Asked: 2018-05-10
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-05-10
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2016-11-18
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2016-11-18
