Anti-NLRP3 Antibody

Product Name

Anti-NLRP3 Antibody

View all NLRP3/NALP3 Antibodies

SKU/Catalog Number

A00034-1

Size

100ul

Form

Liquid

Description

Boster Bio Anti-NLRP3 Antibody catalog # A00034-1. Tested in WB,ICC/IF,IHC,IP,Flow Cytometry applications. This antibody reacts with Human,Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-NLRP3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00034-1)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human Fusin around the phosphorylation site of S339.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Weakly cross-reacts with dog p53.

Reactive Species

A00034-1 is reactive to NLRP3 in Human, Mouse, Rat

Applications

A00034-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

118173 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000
ICC: 1:100-1:500
IF: 1:50-1:200
IHC: 1:50-1:200
FC: 1:50-1:100
IP: 1:50-1:100

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Immunocytochemistry staining of NLRP3 using Anti-NLRP3 Antibody (A00034-1).

ICC staining NLRP3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde

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Figure 2. Immunocytochemistry staining of NLRP3 using Anti-NLRP3 Antibody (A00034-1).

ICC staining NLRP3 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde

Gene/Protein Information For NLRP3 (Source: Uniprot.org, NCBI)

Alternative Names

AGTAVPRL; AII; AII/AVP; Angiotensin/vasopressin receptor AII/AVP-like; mouse NLRP3; NLRP3; AVP; C1orf7; Caterpiller protein 1.1; CIAS1; CLR1.1; Cold autoinflammatory syndrome 1 protein; Cold autoinflammatory syndrome 1; Cryopyrin; Familial cold autoinflammatory syndrome; FCAS; FCU; Leucine-rich repeat-, and PYD-containing protein 3; Muckle-Wells syndrome; MWS; NACHT; NACHT, LRR and PYD containing protein 3; NACHT, LRR and PYD domains-containing protein 3; NALP3; NLR family, pyrin domain containing 3; NLRP3 mouse; NLRP3 polyclonal; NLRP3 rat; NLRP3; Nucleotide-Binding Oligomerization Domain, Le NLRP3 AGTAVPRL, AII, AVP, C1orf7, CIAS1, CLR1.1, DFNA34, FCAS, FCAS1, FCU, KEFH, MWS, NALP3, PYPAF1 NLR family pyrin domain containing 3 NACHT, LRR and PYD domains-containing protein 3|NACHT domain-, leucine-rich repeat-, and PYD-containing protein 3|NACHT, LRR and PYD containing protein 3|PYRIN-containing APAF1-like protein 1|caterpiller protein 1.1|cold autoinflammatory syndrome 1 protein|cold-induced autoinflammatory syndrome 1 protein|cryopyrin|cryopyrin, NACHT, LRR and PYD domains - containing protein 3|deafness, autosomal dominant 34|nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 3

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