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Product Info Summary
| SKU: | A00034-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-NLRP3 Antibody
View all NLRP3/NALP3 Antibodies
SKU/Catalog Number
A00034-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-NLRP3 Antibody catalog # A00034-1. Tested in WB,ICC/IF,IHC,IP,Flow Cytometry applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NLRP3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00034-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
recombinant protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00034-1 is reactive to NLRP3 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
118173 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00034-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
ICC: 1:100-1:500
IF: 1:50-1:200
IHC: 1:50-1:200
FC: 1:50-1:100
IP: 1:50-1:100
Validation Images & Assay Conditions
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Figure 1. Immunocytochemistry staining of NLRP3 using Anti-NLRP3 Antibody (A00034-1).
ICC staining NLRP3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde
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Figure 2. Immunocytochemistry staining of NLRP3 using Anti-NLRP3 Antibody (A00034-1).
ICC staining NLRP3 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehydeProtein Target Info & Infographic
Gene/Protein Information For NLRP3 (Source: Uniprot.org, NCBI)
Gene Name
NLRP3
Full Name
NACHT, LRR and PYD domains-containing protein 3
Weight
118173 MW
Superfamily
NLRP family
Alternative Names
NACHT, LRR and PYD domains-containing protein 3;Angiotensin/vasopressin receptor AII/AVP-like;Caterpiller protein 1.1;CLR1.1;Cold-induced autoinflammatory syndrome 1 protein;Cryopyrin;PYRIN-containing APAF1-like protein 1;NLRP3;C1orf7, CIAS1, NALP3, PYPAF1; NLRP3 AGTAVPRL, AII, AVP, C1orf7, CIAS1, CLR1.1, DFNA34, FCAS, FCAS1, FCU, KEFH, MWS, NALP3, PYPAF1 NLR family pyrin domain containing 3 NACHT, LRR and PYD domains-containing protein 3|NACHT domain-, leucine-rich repeat-, and PYD-containing protein 3|NACHT, LRR and PYD containing protein 3|PYRIN-containing APAF1-like protein 1|caterpiller protein 1.1|cold autoinflammatory syndrome 1 protein|cold-induced autoinflammatory syndrome 1 protein|cryopyrin|cryopyrin, NACHT, LRR and PYD domains - containing protein 3|deafness, autosomal dominant 34|nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NLRP3, check out the NLRP3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NLRP3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NLRP3 Antibody (A00034-1)
Hello CJ!
A00034-1 has been cited in 8 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Xia S,Yang P,Li F,Yu Q,Kuang W,Zhu Y,Lu J,Wu H,Li L,Huang H.Chaihu-Longgu-Muli Decoction Exerts an Antiepileptic Effect in Rats by Improving Pyroptosis in Hippocampal Neurons.J Ethnopharmacol.2021 Jan 7:113794.doi:10.1016/j.jep.2021.113794.Epub ahead of p
Species: Rat
A00034-1 usage in article: APP:IF, SAMPLE:BRAIN TISSUE, DILUTION:1:100
Huang Y,Wang S,Huang F,Zhang Q,Qin B,Liao L,Wang M,Wan H,Yan W,Chen D,Liu F,Jiang B,Ji D,Xia X,Huang J,Xiong K.c-FLIP regulates pyroptosis in retinal neurons following oxygen-glucose deprivation/recovery via a GSDMD-mediated pathway.Ann Anat.2021 Jan 9;23
Species: Rat
A00034-1 usage in article: APP:WB, SAMPLE:R28 RETINAL NEURONAL LIKE CELL AND RETINAL TISSUE, DILUTION:1:800
Zuowei Pei,Ying Guo,Huolan Zhu et al.Thymoquinone protects against hyperlipemia-induced cardiac damage in low-density lipoprotein receptor-deficient (LDL-R−/−) mice,28 April 2020,PREPRINT (Version 1) available at Research Square[https://doi.org/10.21203/r
Species: Mouse
A00034-1 usage in article: APP:WB, SAMPLE:CARDIAC TISSUE, DILUTION:1:1000
Qianrui Zhang,Sheng Zhao,Wenxia Zheng,Haitan Fu,Tao Wu & Fei Hu (2020) Plumbagin attenuated oxygen-glucose deprivation/reoxygenation-induced injury in human SH-SY5Y cells by inhibiting NOX4-derived ROS-activated NLRP3 inflammasome, Bioscience, Biotechnolo
Species: Human
A00034-1 usage in article: APP:WB, SAMPLE:SH-SY5Y CELL, DILUTION:1:200
Hu M,Wu X,Luo M,Wei H,Xu D,Xu F. Lactobacillus rhamnosus FLRH93 protects against intestinal damage in mice induced by 5-fluorouracil. J Dairy Sci. 2020 Jun;103(6):5003-5018.doi:10.3168/jds.2019-17836. Epub 2020 Mar 27.PMID:32229117.
Species: Human,Mouse
A00034-1 usage in article: APP:IHC, SAMPLE:ILEUM TISSUE, DILUTION:1:100
Yang R,Jia Q,Mehmood S,Ma S,Liu X.Genistein ameliorates inflammation and insulin resistance through mediation of gut microbiota composition in type 2 diabetic mice.Eur J Nutr.2020 Oct 17.doi:10.1007/s0 0394-020-02403-0.Epub ahead of print.PMID:33068158.
Species: Mouse
A00034-1 usage in article: APP:IHC, SAMPLE:HEPATIC TISSUE, DILUTION:NA
Liu R,Wu S,Guo C,Hu Z,Peng J,Guo K,Zhang X,Li J.Ibuprofen Exerts Antiepileptic and Neuroprotective Effects in the Rat Model of Pentylenetetrazol-Induced Epilepsy via the COX-2/NLRP3/IL-18 Pathway.Neurochem Res.2020 Oct ;45(10):2516-2526.doi:10.1007/s11064
Species: rat
A00034-1 usage in article: APP:WB, SAMPLE:HIPPOCAMPUL TISSUE, DILUTION:1:1000
Zuo-Wei Pei, Ying Guo, Huo-Lan Zhu, Min Dong, Qian Zhang, Fang Wang, "Thymoquinone Protects against Hyperlipidemia-Induced Cardiac Damage in Low-Density Lipoprotein Receptor-Deficient (LDL-R-/-) Mice via Its Anti-inflammatory and Antipyroptotic Effects",
Species: Mouse
A00034-1 usage in article: APP:WB, SAMPLE:CARDIAC TISSUES, DILUTION:1:1000
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