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Product Info Summary
| SKU: | PA1665 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-CIAS1/NALP3/NLRP3 Antibody Picoband®
View all NLRP3/NALP3 Antibodies
SKU/Catalog Number
PA1665
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CIAS1/NALP3/NLRP3 Antibody catalog # PA1665. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CIAS1/NALP3/NLRP3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1665)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human CIAS1, different from the related rat and mouse sequences by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1665 is reactive to NLRP3 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
110 kDa
Calculated molecular weight
118173 MW
Background of NLRP3/NALP3
NLRP3 (NLR FAMILY, PYRIN DOMAIN-CONTAINING 3), also known as CIAS1, CRYOPYRIN, NALP3 or PYPAF1, is a protein that in humans is encoded by the NLRP3 (NOD-like receptor family, pryin domain containing 3) gene. The NLRP3 gene encodes a pyrin-like protein expressed predominantly in peripheral blood leukocytes. And the NLRP3 gene is mapped on 1q44. NLRP3 interacts with apoptosis-associated speck-like protein containing a CARD (ASC). The encoded protein may play a role in the regulation of inflammation and apoptosis. Mutation of the NALP3 nucleotide-binding domain reduced ATP binding, CASP1 activation, IL1B production, cell death, macromolecular complex formation, self-association, and association with ASC. Consistent with an essential role for Nlrp3 inflammasomes in antifungal immunity, Gross et al.showed that Nlrp3-deficient mice are hypersusceptible to C. albicans infection. Activation of the NLRP3 inflammasome in response to virus or to RNA was dependent upon lysosomal maturation and reactive oxygen species production in human cells. The NLRP3 inflammasome senses obesity-associated danger signals and contributes to obesity-induced inflammation and insulin resistance.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1665 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, By Heat
Flow Cytometry(Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: human THP-1 whole cell, rat PC-12 whole cell, mouse spleen whole cell, mouse RAW2647 whole cell
IHC: human tonsil tissue, human squamous cell cervical carcinoma tissue
FCM: THP-1 cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of NLRP3 using anti-NLRP3 antibody (PA1665).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates,
Lane 2: rat PC-12 whole cell lysates,
Lane 3: mouse spleen whole cell lysates,
Lane 4: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NLRP3 antigen affinity purified polyclonal antibody (Catalog # PA1665) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NLRP3 at approximately 110 kDa. The expected band size for NLRP3 is at 118 kDa.
Click image to see more details
Figure 2. IHC analysis of NLRP3 using anti-NLRP3 antibody (PA1665).
NLRP3 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NLRP3 Antibody (PA1665) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of NLRP3 using anti-NLRP3 antibody (PA1665).
NLRP3 was detected in a paraffin-embedded section of human squamous cell cervical carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NLRP3 Antibody (PA1665) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 4. Flow Cytometry analysis of THP-1 cells using anti-NLRP3 antibody (PA1665).
Overlay histogram showing THP-1 cells stained with PA1665 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NLRP3 Antibody (PA1665, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For NLRP3 (Source: Uniprot.org, NCBI)
Gene Name
NLRP3
Full Name
NACHT, LRR and PYD domains-containing protein 3
Weight
118173 MW
Superfamily
NLRP family
Alternative Names
NACHT, LRR and PYD domains-containing protein 3;Angiotensin/vasopressin receptor AII/AVP-like;Caterpiller protein 1.1;CLR1.1;Cold autoinflammatory syndrome 1 protein;Cryopyrin;PYRIN-containing APAF1-like protein 1;NLRP3;C1orf7, CIAS1, NALP3, PYPAF1; NLRP3 AGTAVPRL, AII, AVP, C1orf7, CIAS1, CLR1.1, DFNA34, FCAS, FCAS1, FCU, KEFH, MWS, NALP3, PYPAF1 NLR family pyrin domain containing 3 NACHT, LRR and PYD domains-containing protein 3|NACHT domain-, leucine-rich repeat-, and PYD-containing protein 3|NACHT, LRR and PYD containing protein 3|PYRIN-containing APAF1-like protein 1|caterpiller protein 1.1|cold autoinflammatory syndrome 1 protein|cold-induced autoinflammatory syndrome 1 protein|cryopyrin|cryopyrin, NACHT, LRR and PYD domains - containing protein 3|deafness, autosomal dominant 34|nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NLRP3, check out the NLRP3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NLRP3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CIAS1/NALP3/NLRP3 Antibody Picoband® (PA1665)
Hello CJ!
PA1665 has been cited in 18 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Pelargonic acid vanillylamide and rosuvastatin protect against oxidized low-density lipoprotein-induced endothelial dysfunction by inhibiting the NF-κB/NLRP3 pathway and improving cell-cell junctions
The combination of dapagliflozin and statins ameliorates renal injury through attenuating the activation of inflammasome-mediated autophagy in insulin-resistant rats
Sivasinprasasn S,Wikan N,Tocharus J,Chaichompoo W,Suksamrarn A,Tocharus C.Pelargonic acid vanillylamide and rosuvastatin protect against oxidized low-density lipoprotein-induced endothelial dysfunction by inhibiting the NF-κB/NLRP3 pathway and improving cell-cell junctions.Chem Biol Interact.2021 Jul 1:109572.doi:10.1016/j.cbi.2021.109572.Epub ahead of print.PMID:34217687.
Species: Human
PA1665 usage in article: APP:WB, SAMPLE:HUVEC CELL, DILUTION:1:1000
Huiqing Zhang,Yuna Du,Yujie Guo,Zeyu Wang,Hua Li,Zhe Lv,Lifeng Zeng,Yiguo Chen,Zhengyu Xie,Rong Li,"TLR4-NLRP3-GSDMD-Mediated Pyroptosis Plays an Important Role in Aggravated Liver Injury of CD38-/- Sepsis Mice",Journal of Immunology Research,vol.2021,Article ID 6687555,15 pages,2021.https://doi.org/10.1155/2021/6687555
Species: Mouse
PA1665 usage in article: APP:WB, SAMPLE:LIVER TISSUE, DILUTION:1:500
Lei L,Guo Y,Lin J,Lin X,He S,Qin Z,Lin Q. Inhibition of endotoxin-induced acute lung injury in rats by bone marrow-derived mesenchymal stem cells: Role of Nrf2/HO-1 signal axis in inhibition of NLRP3 activation. Biochem Biophys Res Commun.2021 Mar 10;551:7-13.doi:10.1016/j.bbrc.2021.03.009.Epub ahead of print.PMID: 33713981.
Species: Rat
PA1665 usage in article: APP:WB, SAMPLE:LUNG TISSUE, DILUTION:1:1000
Du X,Xu Y,Chen S,Fang M.Inhibited CSF1R Alleviates Ischemia Injury via Inhibition of Microglia M1 Polarization and NLRP3 Pathway.Neural Plast.2020 Aug 28;2020:8825954.doi:10.1155/2020/8825954.PMID:32908485;PMCID:PMC7474788.
Species: Mouse
PA1665 usage in article: APP:IF, SAMPLE:BRAIN TISSUE, DILUTION:1:100
Hou J,Lei Z,Cui L,Hou Y,Yang L,An R,Wang Q,Li S,Zhang H,Zhang L.Polystyrene microplastics lead to pyroptosis and apoptosis of ovarian granulosa cells via NLRP3/Caspase-1 signaling pathway in rats.Ecotoxicol Environ Saf.2021 Feb 4;212:112012.doi:10.1016/j.
Species: Rat
PA1665 usage in article: APP:WB, SAMPLE:OVARY AND GRANULOSA CELL, DILUTION:1:300
Xia S,Yang P,Li F,Yu Q,Kuang W,Zhu Y,Lu J,Wu H,Li L,Huang H.Chaihu-Longgu-Muli Decoction Exerts an Antiepileptic Effect in Rats by Improving Pyroptosis in Hippocampal Neurons.J Ethnopharmacol.2021 Jan 7:113794.doi:10.1016/j.jep.2021.113794.Epub ahead of p
Species: Rat
PA1665 usage in article: APP:IF, SAMPLE:BRAIN TISSUE, DILUTION:1:100
Huang Y,Wang S,Huang F,Zhang Q,Qin B,Liao L,Wang M,Wan H,Yan W,Chen D,Liu F,Jiang B,Ji D,Xia X,Huang J,Xiong K.c-FLIP regulates pyroptosis in retinal neurons following oxygen-glucose deprivation/recovery via a GSDMD-mediated pathway.Ann Anat.2021 Jan 9;23
Species: Rat
PA1665 usage in article: APP:WB, SAMPLE:R28 RETINAL NEURONAL LIKE CELL AND RETINAL TISSUE, DILUTION:1:800
Zuowei Pei,Ying Guo,Huolan Zhu et al.Thymoquinone protects against hyperlipemia-induced cardiac damage in low-density lipoprotein receptor-deficient (LDL-R−/−) mice,28 April 2020,PREPRINT (Version 1) available at Research Square[https://doi.org/10.21203/r
Species: Mouse
PA1665 usage in article: APP:WB, SAMPLE:CARDIAC TISSUE, DILUTION:1:1000
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Customer Reviews
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Customer Q&As
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18 Customer Q&As for Anti-CIAS1/NALP3/NLRP3 Antibody Picoband®
Question
Do you have a BSA free version of anti-CIAS1/NALP3/NLRP3 antibody PA1665 available?
Verified Customer
Verified customer
Asked: 2020-04-09
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CIAS1/NALP3/NLRP3 antibody PA1665 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-09
Question
Is this PA1665 anti-CIAS1/NALP3/NLRP3 antibody reactive to the isotypes of NLRP3?
Verified Customer
Verified customer
Asked: 2020-04-02
Answer
The immunogen of PA1665 anti-CIAS1/NALP3/NLRP3 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human CIAS1(12-31aa RYLEDLEDVDLKKFKMHLED), different from the related rat and mouse sequences by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-02
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for umbilical cord blood using anti-CIAS1/NALP3/NLRP3 antibody PA1665. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-16
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-16
Question
We have seen staining in rat blood. Are there any suggestions? Is anti-CIAS1/NALP3/NLRP3 antibody supposed to stain blood positively?
Verified Customer
Verified customer
Asked: 2020-02-06
Answer
From what I have seen in literature blood does express NLRP3. From what I have seen in Uniprot.org, NLRP3 is expressed in blood, brain, colon, umbilical cord blood, among other tissues. Regarding which tissues have NLRP3 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14702039, 16710414
Colon, Pubmed ID: 15489334
Umbilical cord blood, Pubmed ID: 11042152
Boster Scientific Support
Answered: 2020-02-06
Question
We purchased anti-CIAS1/NALP3/NLRP3 antibody for Flow Cytometry on brain a few years ago. I am using mouse, and We are going to use the antibody for ICC next. I would like examining brain as well as blood in our next experiment. Could you please give me some suggestion on which antibody would work the best for ICC?
Verified Customer
Verified customer
Asked: 2020-01-16
Answer
I have checked the website and datasheets of our anti-CIAS1/NALP3/NLRP3 antibody and I see that PA1665 has been validated on mouse in both Flow Cytometry and ICC. Thus PA1665 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-01-16
Question
Does PA1665 anti-CIAS1/NALP3/NLRP3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-28
Answer
As indicated on the product datasheet, PA1665 anti-CIAS1/NALP3/NLRP3 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-28
Question
Does anti-CIAS1/NALP3/NLRP3 antibody PA1665 work for WB with umbilical cord blood?
Verified Customer
Verified customer
Asked: 2019-11-27
Answer
According to the expression profile of umbilical cord blood, NLRP3 is highly expressed in umbilical cord blood. So, it is likely that anti-CIAS1/NALP3/NLRP3 antibody PA1665 will work for WB with umbilical cord blood.
Boster Scientific Support
Answered: 2019-11-27
Question
I see that the anti-CIAS1/NALP3/NLRP3 antibody PA1665 works with WB, what is the protocol used to produce the result images on the product page?
L. Taylor
Verified customer
Asked: 2019-10-29
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-29
Question
I am looking for to test anti-CIAS1/NALP3/NLRP3 antibody PA1665 on human umbilical cord blood for research purposes, then I may be interested in using anti-CIAS1/NALP3/NLRP3 antibody PA1665 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-18
Answer
The products we sell, including anti-CIAS1/NALP3/NLRP3 antibody PA1665, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-18
Question
I am interested in using your anti-CIAS1/NALP3/NLRP3 antibody for positive regulation of interleukin-13 production studies. Has this antibody been tested with western blotting on u87 cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-10-08
Answer
I appreciate your inquiry. This PA1665 anti-CIAS1/NALP3/NLRP3 antibody is tested on a549 cell lysate, u87 cell lysate, cem cell lysate, u937 cells, rat kidney. It is guaranteed to work for Flow Cytometry, IF, IHC-P, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-10-08
Question
My question regarding product PA1665, anti-CIAS1/NALP3/NLRP3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-23
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1665 anti-CIAS1/NALP3/NLRP3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-23
Question
I was wanting to use your anti-CIAS1/NALP3/NLRP3 antibody for WB for human umbilical cord blood on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human umbilical cord blood identification?
Verified Customer
Verified customer
Asked: 2019-07-16
Answer
It shows on the product datasheet, PA1665 anti-CIAS1/NALP3/NLRP3 antibody has been tested for Flow Cytometry, IF, IHC-P, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human umbilical cord blood in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-16
Question
Does anti-CIAS1/NALP3/NLRP3 antibody PA1665 work on primate IHC-P with colon?
Verified Customer
Verified customer
Asked: 2019-05-16
Answer
Our lab technicians have not tested anti-CIAS1/NALP3/NLRP3 antibody PA1665 on primate. You can run a BLAST between primate and the immunogen sequence of anti-CIAS1/NALP3/NLRP3 antibody PA1665 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate colon in IHC-P, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-16
Question
We were happy with the WB result of your anti-CIAS1/NALP3/NLRP3 antibody. However we have been able to see positive staining in blood golgi apparatus membrane. note=(microbial using this antibody. Is that expected? Could you tell me where is NLRP3 supposed to be expressed?
R. Banerjee
Verified customer
Asked: 2019-03-19
Answer
From literature, blood does express NLRP3. Generally NLRP3 expresses in cytoplasm, cytosol, golgi apparatus membrane. note=(microbial. Regarding which tissues have NLRP3 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14702039, 16710414
Colon, Pubmed ID: 15489334
Umbilical cord blood, Pubmed ID: 11042152
Boster Scientific Support
Answered: 2019-03-19
Question
Hello, I currently trying to establish NLRP3 staining (IF) in cryo sections from colon using at the moment an polyclonal NLRP3 antibody from abcam (ab4207, goat). My current antibody shows unspecific staining of colon mucus and epithelia. Did you find a way to counteract this? Is it possible with your aNLRP3-Antibody, to stain NLRP3 specific in cryo colon sections?
Verified Customer
Verified customer
Asked: 2018-10-08
Answer
We are uncertain if PA1665 is specific in cryo colon sections. Please suggest the customer to purchase a sample size to test.
Boster Scientific Support
Answered: 2018-10-08
Question
Is a blocking peptide available for product anti-CIAS1/NALP3/NLRP3 antibody (PA1665)?
M. Edwards
Verified customer
Asked: 2016-08-09
Answer
We do provide the blocking peptide for product anti-CIAS1/NALP3/NLRP3 antibody (PA1665). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-08-09
Question
We are currently using anti-CIAS1/NALP3/NLRP3 antibody PA1665 for human tissue, and we are content with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on feline tissues as well?
J. Roberts
Verified customer
Asked: 2015-09-18
Answer
The anti-CIAS1/NALP3/NLRP3 antibody (PA1665) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-09-18
Question
See below the WB image, lot number and protocol we used for umbilical cord blood using anti-CIAS1/NALP3/NLRP3 antibody PA1665. Please let me know if you require anything else.
E. Evans
Verified customer
Asked: 2014-01-29
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-01-29
