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Product Info Summary
| SKU: | M00034 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-NALP3 NLRP3 Rabbit Monoclonal Antibody
View all NLRP3/NALP3 Antibodies
SKU/Catalog Number
M00034
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-NALP3 NLRP3 Rabbit Monoclonal Antibody catalog # M00034. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NALP3 NLRP3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00034)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
FBD-14
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human NALP3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00034 is reactive to NLRP3 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
110 kDa
Calculated molecular weight
88068 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00034 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
IHC: human lung carcinoma tissue
ICC/IF: Raw2647 cell
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Western blot analysis of NLRP3 expression in SH-SY5Y cell lysate.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using NALP3 Antibody.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Immunofluorescent analysis of Raw264.7 cells, using NALP3 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For NLRP3 (Source: Uniprot.org, NCBI)
Gene Name
NLRP3
Full Name
NACHT, LRR and PYD domains-containing protein 3
Weight
88068 MW
Superfamily
NLRP family
Alternative Names
Signal transducer and activator of transcription 3;Acute-phase response factor;STAT3;APRF; NLRP3 AGTAVPRL, AII, AVP, C1orf7, CIAS1, CLR1.1, DFNA34, FCAS, FCAS1, FCU, KEFH, MWS, NALP3, PYPAF1 NLR family pyrin domain containing 3 NACHT, LRR and PYD domains-containing protein 3|NACHT domain-, leucine-rich repeat-, and PYD-containing protein 3|NACHT, LRR and PYD containing protein 3|PYRIN-containing APAF1-like protein 1|caterpiller protein 1.1|cold autoinflammatory syndrome 1 protein|cold-induced autoinflammatory syndrome 1 protein|cryopyrin|cryopyrin, NACHT, LRR and PYD domains - containing protein 3|deafness, autosomal dominant 34|nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NLRP3, check out the NLRP3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NLRP3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NALP3 NLRP3 Rabbit Monoclonal Antibody (M00034)
Hello CJ!
M00034 has been cited in 14 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Mangiferin inhibits lipopolysaccharide‐induced epithelial‐mesenchymal transition (EMT) and enhances the expression of tumor suppressor gene PER1 in non‐small cell lung cancer cells
Wei Z,Wang J,Wang Y,Wang C,Liu X,Han Z,Fu Y,Yang Z.Effects of Neutrophil Extracellular Traps on Bovine Mammary Epithelial Cells in vitro.Front Immunol.2019 May 17;10:1003.doi:10.3389/fimmu.2019.01003.PMID:31156617;PMCID:PMC6533846.
Species: Cows
M00034 usage in article: APP:WB, SAMPLE:BMECS, DILUTION:NA
Huiqing Zhang,Yuna Du,Yujie Guo,Zeyu Wang,Hua Li,Zhe Lv,Lifeng Zeng,Yiguo Chen,Zhengyu Xie,Rong Li,"TLR4-NLRP3-GSDMD-Mediated Pyroptosis Plays an Important Role in Aggravated Liver Injury of CD38-/- Sepsis Mice",Journal of Immunology Research,vol.2021,Article ID 6687555,15 pages,2021.https://doi.org/10.1155/2021/6687555
Species: Mouse
M00034 usage in article: APP:WB, SAMPLE: LIVER TISSUE, DILUTION:1:500
Du X,Xu Y,Chen S,Fang M.Inhibited CSF1R Alleviates Ischemia Injury via Inhibition of Microglia M1 Polarization and NLRP3 Pathway.Neural Plast.2020 Aug 28;2020:8825954.doi:10.1155/2020/8825954.PMID:32908485;PMCID:PMC7474788.
Species: Mouse
M00034 usage in article: APP:IF, SAMPLE:BRAIN TISSUE, DILUTION:1:100
Hou J,Lei Z,Cui L,Hou Y,Yang L,An R,Wang Q,Li S,Zhang H,Zhang L.Polystyrene microplastics lead to pyroptosis and apoptosis of ovarian granulosa cells via NLRP3/Caspase-1 signaling pathway in rats.Ecotoxicol Environ Saf.2021 Feb 4;212:112012.doi:10.1016/j.
Species: Rat
M00034 usage in article: APP:IHC, SAMPLE:OVARY TISSUE, DILUTION:1:300
Xia S,Yang P,Li F,Yu Q,Kuang W,Zhu Y,Lu J,Wu H,Li L,Huang H.Chaihu-Longgu-Muli Decoction Exerts an Antiepileptic Effect in Rats by Improving Pyroptosis in Hippocampal Neurons.J Ethnopharmacol.2021 Jan 7:113794.doi:10.1016/j.jep.2021.113794.Epub ahead of p
Species: Rat
Huang Y,Wang S,Huang F,Zhang Q,Qin B,Liao L,Wang M,Wan H,Yan W,Chen D,Liu F,Jiang B,Ji D,Xia X,Huang J,Xiong K.c-FLIP regulates pyroptosis in retinal neurons following oxygen-glucose deprivation/recovery via a GSDMD-mediated pathway.Ann Anat.2021 Jan 9;23
Species: Rat
M00034 usage in article: APP:WB, SAMPLE:R28 RETINAL NEURONAL LIKE CELL AND RETINAL TISSUE, DILUTION:1:800
Zuowei Pei,Ying Guo,Huolan Zhu et al.Thymoquinone protects against hyperlipemia-induced cardiac damage in low-density lipoprotein receptor-deficient (LDL-R−/−) mice,28 April 2020,PREPRINT (Version 1) available at Research Square[https://doi.org/10.21203/r
Species: Mouse
M00034 usage in article: APP:WB, SAMPLE:CARDIAC TISSUE, DILUTION:1:1000
Qianrui Zhang,Sheng Zhao,Wenxia Zheng,Haitan Fu,Tao Wu & Fei Hu (2020) Plumbagin attenuated oxygen-glucose deprivation/reoxygenation-induced injury in human SH-SY5Y cells by inhibiting NOX4-derived ROS-activated NLRP3 inflammasome, Bioscience, Biotechnolo
Species: Human
M00034 usage in article: APP:WB, SAMPLE:SH-SY5Y CELL, DILUTION:1:200
Hu M,Wu X,Luo M,Wei H,Xu D,Xu F. Lactobacillus rhamnosus FLRH93 protects against intestinal damage in mice induced by 5-fluorouracil. J Dairy Sci. 2020 Jun;103(6):5003-5018.doi:10.3168/jds.2019-17836. Epub 2020 Mar 27.PMID:32229117.
Species: Human,Mouse
M00034 usage in article: APP:IHC, SAMPLE:ILEUM TISSUE, DILUTION:1:100
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Customer Reviews
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Customer Q&As
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17 Customer Q&As for Anti-NALP3 NLRP3 Rabbit Monoclonal Antibody
Question
I was wanting to use your anti-NALP3 Monoclonal antibody for WB for human umbilical cord blood on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human umbilical cord blood identification?
Verified Customer
Verified customer
Asked: 2020-04-14
Answer
It shows on the product datasheet, M00034 anti-NALP3 Monoclonal antibody has been tested for IP, IF, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human umbilical cord blood in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-14
Question
Is this M00034 anti-NALP3 Monoclonal antibody reactive to the isotypes of NLRP3?
Verified Customer
Verified customer
Asked: 2020-03-04
Answer
The immunogen of M00034 anti-NALP3 Monoclonal antibody is A synthesized peptide derived from human NALP3. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-04
Question
Would M00034 anti-NALP3 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-24
Answer
You can see on the product datasheet, M00034 anti-NALP3 Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-24
Question
We are currently using anti-NALP3 Monoclonal antibody M00034 for human tissue, and we are satisfied with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-20
Answer
The anti-NALP3 Monoclonal antibody (M00034) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-20
Question
See below the WB image, lot number and protocol we used for umbilical cord blood using anti-NALP3 Monoclonal antibody M00034. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-10
Question
Do you have a BSA free version of anti-NALP3 Monoclonal antibody M00034 available?
Verified Customer
Verified customer
Asked: 2019-08-07
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-NALP3 Monoclonal antibody M00034 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-08-07
Question
I see that the anti-NALP3 Monoclonal antibody M00034 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-15
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-15
Question
Can you help my question with product M00034, anti-NALP3 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-30
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00034 anti-NALP3 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-30
Question
I am interested in to test anti-NALP3 Monoclonal antibody M00034 on human umbilical cord blood for research purposes, then I may be interested in using anti-NALP3 Monoclonal antibody M00034 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-05-22
Answer
The products we sell, including anti-NALP3 Monoclonal antibody M00034, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-22
Question
We need using your anti-NALP3 Monoclonal antibody for positive regulation of t-helper 17 cell differentiation studies. Has this antibody been tested with western blotting on raw264.7 cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-01-10
Answer
Thanks for your inquiry. This M00034 anti-NALP3 Monoclonal antibody is validated on raw264.7 cells. It is guaranteed to work for IP, IF, IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-01-10
Question
Would anti-NALP3 Monoclonal antibody M00034 work for WB with umbilical cord blood?
Verified Customer
Verified customer
Asked: 2018-09-10
Answer
According to the expression profile of umbilical cord blood, NLRP3 is highly expressed in umbilical cord blood. So, it is likely that anti-NALP3 Monoclonal antibody M00034 will work for WB with umbilical cord blood.
Boster Scientific Support
Answered: 2018-09-10
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for umbilical cord blood using anti-NALP3 Monoclonal antibody M00034. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-01-29
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-01-29
Question
Our team were satisfied with the WB result of your anti-NALP3 Monoclonal antibody. However we have observed positive staining in brain cytoplasm using this antibody. Is that expected? Could you tell me where is NLRP3 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-11-01
Answer
According to literature, brain does express NLRP3. Generally NLRP3 expresses in cytoplasm, cytosol, golgi apparatus membrane. note=(microbial. Regarding which tissues have NLRP3 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14702039, 16710414
Colon, Pubmed ID: 15489334
Umbilical cord blood, Pubmed ID: 11042152
Boster Scientific Support
Answered: 2017-11-01
Question
We purchased anti-NALP3 Monoclonal antibody for IP on blood in a previous experiment. I am using mouse, and We want to use the antibody for IHC next. My lab would like examining blood as well as brain in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
M. Parker
Verified customer
Asked: 2016-04-21
Answer
I looked at the website and datasheets of our anti-NALP3 Monoclonal antibody and it seems that M00034 has been validated on mouse in both IP and IHC. Thus M00034 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2016-04-21
Question
Is a blocking peptide available for product anti-NALP3 Monoclonal antibody (M00034)?
B. Li
Verified customer
Asked: 2014-07-23
Answer
We do provide the blocking peptide for product anti-NALP3 Monoclonal antibody (M00034). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-07-23
Question
We have been able to see staining in human brain. What should we do? Is anti-NALP3 Monoclonal antibody supposed to stain brain positively?
H. Brown
Verified customer
Asked: 2013-12-24
Answer
According to literature brain does express NLRP3. According to Uniprot.org, NLRP3 is expressed in blood, brain, colon, umbilical cord blood, among other tissues. Regarding which tissues have NLRP3 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14702039, 16710414
Colon, Pubmed ID: 15489334
Umbilical cord blood, Pubmed ID: 11042152
Boster Scientific Support
Answered: 2013-12-24
Question
A customer called and wanted to confirm the clone number for
Verified Customer
Verified customer
Asked: 2013-02-25
Answer
The clone number for Anti-NALP3 Monoclonal Antibody M00034 is FBD-14. This antibody isn't validated for IHC-F. Abways suggest the customer run a pilot IHC-F test if the customer wants to have a try.
Boster Scientific Support
Answered: 2013-02-25
