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Product Info Summary
| SKU: | A03001-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-Ninein/NIN Antibody Picoband®
SKU/Catalog Number
A03001-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-Ninein/NIN Antibody Picoband® catalog # A03001-1. Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Ninein/NIN Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03001-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Ninein/NIN recombinant protein (Position: Q1023-H2090).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03001-1 is reactive to NIN in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
260 kDa
Calculated molecular weight
243.718kDa
Background of Ninein
Ninein is a protein that in humans is encoded by the NIN gene. This gene encodes one of the proteins important for centrosomal function. This protein is important for positioning and anchoring the microtubules minus-ends in epithelial cells. Localization of this protein to the centrosome requires three leucine zippers in the central coiled-coil domain. Multiple alternatively spliced transcript variants that encode different isoforms have been reported.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03001-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human
ELISA, 0.1-0.5 µg/ml, Human
Positive Control
WB: human Hela whole cell, human MOLT4 whole cell, human MCF-7 whole cell, human U20S whole cell, rat heart tissue, mouse heart tissue
FCM: MCF-7 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Ninein/NIN using anti-Ninein/NIN antibody (A03001-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MOLT4 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human U20S whole cell lysates,
Lane 5: rat heart tissue lysates,
Lane 6: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ninein/NIN antigen affinity purified polyclonal antibody (Catalog # A03001-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ninein/NIN at approximately 260 kDa. The expected band size for Ninein/NIN is at 243 kDa.
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Figure 2. Flow Cytometry analysis of MCF-7 cells using anti-Ninein/NIN antibody (A03001-1).
Overlay histogram showing MCF-7 cells stained with A03001-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Ninein/NIN Antibody (A03001-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For NIN (Source: Uniprot.org, NCBI)
Gene Name
NIN
Full Name
Ninein
Weight
243.718kDa
Alternative Names
Endoglin; Cell surface MJ7/18 antigen; CD105; Eng; Edg; Nin|3110068G20Rik, AI385615, AU024711, mKIAA1565|ninein|ninein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NIN, check out the NIN Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NIN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Ninein/NIN Antibody Picoband® (A03001-1)
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