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Product Info Summary
| SKU: | M06821-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Bovine, Chicken, Equine, Human, Mouse, Pig, Rat |
| Host: | Mouse |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-Neurofilament Medium NF-M Monoclonal Antibody
SKU/Catalog Number
M06821-1
Size
100ul
Description
Boster Bio Anti-Neurofilament Medium NF-M Monoclonal Antibody catalog # M06821-1. Tested in IF, IHC, WB applications. This antibody reacts with Bovine, Chicken, Equine, Human, Mouse, Pig, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Neurofilament Medium NF-M Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06821-1)
Host
Mouse
Contents
Affinity purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3
Clonality
Monoclonal
Immunogen
Recombinant fusion protein containing the extreme C-terminus of rat NF-M, amino acids 677-845, expressed in and purified from E. coli
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M06821-1 is reactive to NEFM in Bovine, Chicken, Equine, Human, Mouse, Pig, Rat
Reconstitution
Calculated molecular weight
102472 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06821-1 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:5,000. IF/ICC and IHC: 1:500.
Validation Images & Assay Conditions
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Western blot analysis of neuronal tissue lysates using mouse mAb to NF-M, M06821-1, dilution 1:10,000 in green: [1] protein standard (red), [2] rat spinal cord, [3] mouse spinal cord, [4] cow spinal cord, [5] rat sciatic nerve. Strong bands at 145kDa correspond to rodent NF-M while that at about 160kDa corresponds to the significantly larger bovine NF-M protein.
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Immunofluorescence analysis of adult rat frontal cortex section stained with mouse mAb to neurofilament NF-M, M06821-1, dilution 1:5,000 in green, and costained with chicken pAb to neurofilament NF-H, dilution 1:5,000 in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45M, and free-floating sections were stained with above antibodies. M06821-1 antibody labels neuron cell bodies and dendrites of pyramidal neurons, as well as dendrites and axons of other neuronal cells, while the NF-H antibody stains the network of neuronal axons only.
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Chromogenic immunostaining of a 4% PFA fixed paraffin embedded rat cerebellum section with mouse mAb to NF-M, M06821-1, dilution 1:10,000, detected with DAB (brown) using the Vector Labs ImmPRESS method and reagents with citra buffer retrieval. Hematoxylin (blue) was used as the counterstain. This antibody performs well in testing with both 4% PFA and standard NBF fixed tissues but does not stain long term NBF fixed tissue effectively.
Protein Target Info & Infographic
Gene/Protein Information For NEFM (Source: Uniprot.org, NCBI)
Gene Name
NEFM
Full Name
Neurofilament medium polypeptide
Weight
102472 MW
Superfamily
intermediate filament family
Alternative Names
Neurofilament medium polypeptide;NF-M;160 kDa neurofilament protein;Neurofilament 3;Neurofilament triplet M protein;NEFM;NEF3, NFM; NEFM NEF3, NF-M, NFM neurofilament medium neurofilament medium polypeptide|160 kDa neurofilament protein|neurofilament 3|neurofilament triplet M protein|neurofilament, medium polypeptide 150kDa|neurofilament-3 (150 kD medium)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NEFM, check out the NEFM Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NEFM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Neurofilament Medium NF-M Monoclonal Antibody (M06821-1)
Hello CJ!
M06821-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Wu K,Yue J,Shen K,He J,Zhu G,Liu S,Yang H,Zhang C.Expression and cellular distribution of FGF13 in cortical tubers of the tuberous sclerosis complex.Neurosci Lett. 2021 Feb 11:135714.doi:10.1016/j.neulet.2021.135714.Epub ahead of print.PMID:33582188.
Species: Human
M06821-1 usage in article: APP:IF, SAMPLE:BRAIN TISSUE, DILUTION:1:50
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