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Product Info Summary
| SKU: | M06821-3 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Bovine, Chicken, Equine, Pig |
| Host: | Chicken |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-Neurofilament NF-M Antibody
SKU/Catalog Number
M06821-3
Size
100ul
Description
Boster Bio Anti-Neurofilament NF-M Antibody catalog # M06821-3. Tested in IF, IHC, WB applications. This antibody reacts with Bovine, Chicken, Equine, Pig.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Neurofilament NF-M Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06821-3)
Host
Chicken
Contents
Purified IgY preparation at 20-30 mg/mL with 5mM NaN3
Clonality
Polyclonal
Immunogen
Recombinant construct containing the C-terminus of the human sequence (amino acids 708-877) expressed in and purified from E. coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M06821-3 is reactive to NEFM in Bovine, Chicken, Equine, Pig
Reconstitution
Calculated molecular weight
102472 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06821-3 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Use 1:10,000 for WB, 1:1,000 for ICC
Validation Images & Assay Conditions
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Western blot analysis of different neuronal tissue and cell lysates using chicken pAb to NF-M, M06821-3, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] NIH/3T3 cells, [7] HEK293, [8] HeLa, [9] SH-SY5Y, and [10] C6 cells. Strong band at 145kDa corresponds to rodent NF-M, and about 160kDa band corresponds to human NF-M protein, visible in SHSY-5Y and HEK293 cells which have neuronal properties. NF-M is not expressed in HeLa and other cell lines tested.
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Immunofluorescent analysis of rat cerebellum section stained with chicken pAb to NF-M, M06821-3, dilution 1:1,000 in red, and costained with mouse mAb to CNP, dilution 1:500 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45M, and free-floating sections were stained with the above antibodies. The NF-M antibody labels the network of axons of basket neurons and other neurons. The CNP antibody stains oligodendrocytes, cells that create myelin sheaths around axons.
Protein Target Info & Infographic
Gene/Protein Information For NEFM (Source: Uniprot.org, NCBI)
Gene Name
NEFM
Full Name
Neurofilament medium polypeptide
Weight
102472 MW
Superfamily
intermediate filament family
Alternative Names
Neurofilament medium polypeptide;NF-M;160 kDa neurofilament protein;Neurofilament 3;Neurofilament triplet M protein;NEFM;NEF3, NFM; NEFM NEF3, NF-M, NFM neurofilament medium neurofilament medium polypeptide|160 kDa neurofilament protein|neurofilament 3|neurofilament triplet M protein|neurofilament, medium polypeptide 150kDa|neurofilament-3 (150 kD medium)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NEFM, check out the NEFM Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NEFM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Neurofilament NF-M Antibody (M06821-3)
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4 Customer Q&As for Anti-Neurofilament NF-M Antibody
Question
I have a question about product M06821-3, anti-Neurofilament NF-M antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-06
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M06821-3 anti-Neurofilament NF-M antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-06
Question
We are currently using anti-Neurofilament NF-M antibody M06821-3 for pig tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says bovine, chicken, equine, pig. Is it true that the antibody can work on feline tissues as well?
A. Rodriguez
Verified customer
Asked: 2018-03-09
Answer
The anti-Neurofilament NF-M antibody (M06821-3) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-03-09
Question
Is this M06821-3 anti-Neurofilament NF-M antibody reactive to the isotypes of NEFM?
Verified Customer
Verified customer
Asked: 2017-10-05
Answer
The immunogen of M06821-3 anti-Neurofilament NF-M antibody is Recombinant construct containing the C-terminus of the human sequence (amino acids 708-877) expressed in and purified from E. coli.. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-10-05
Question
Would anti-Neurofilament NF-M antibody M06821-3 work for IF with retina?
T. Johnson
Verified customer
Asked: 2017-07-05
Answer
According to the expression profile of retina, NEFM is highly expressed in retina. So, it is likely that anti-Neurofilament NF-M antibody M06821-3 will work for IF with retina.
Boster Scientific Support
Answered: 2017-07-05
