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Product Info Summary
| SKU: | M06821-2 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Bovine, Chicken, Equine, Human, Mouse, Pig, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Neurofilament NF-M Antibody
SKU/Catalog Number
M06821-2
Size
100ul
Description
Boster Bio Anti-Neurofilament NF-M Antibody catalog # M06821-2. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Bovine, Chicken, Equine, Human, Mouse, Pig, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Neurofilament NF-M Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06821-2)
Host
Rabbit
Contents
Supplied as an aliquot of serum plus 5mM NaN3
Clonality
Polyclonal
Immunogen
Recombinant fusion protein containing the extreme C-terminal segment of rat NF-M, amino acids 549-845
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M06821-2 is reactive to NEFM in Bovine, Chicken, Equine, Human, Mouse, Pig, Rat
Reconstitution
Calculated molecular weight
102472 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06821-2 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000-5,000. IF/ICC and IHC: 1:1,000-1:2500. ABC: 1:5,000.
Validation Images & Assay Conditions
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Western blot analysis of neuronal tissue lysates using rabbit pAb to NF-M, M06821-2, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] pig brain, [7] pig spinal cord. Strong bands at 145kDa correspond to rodent NF-M molecules, while the NF-M of pig and other larger mammals including humans run at about 160kDa.
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Immunofluorescent analysis of rat cerebellum section stained with rabbit pAb to NF-M, M06821-2, dilution 1:2,000 in red, and costained with mouse mAb to GAP43, dilution 1:2,000 in green. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45M, and free-floating sections were stained with the above antibodies. The M06821-2 antibody strongly labels neuronal processes throughout the cerebellum, while the GAP43 antibody stains predominantly synaptic regions in the molecular layer.
Protein Target Info & Infographic
Gene/Protein Information For NEFM (Source: Uniprot.org, NCBI)
Gene Name
NEFM
Full Name
Neurofilament medium polypeptide
Weight
102472 MW
Superfamily
intermediate filament family
Alternative Names
Neurofilament medium polypeptide;NF-M;160 kDa neurofilament protein;Neurofilament 3;Neurofilament triplet M protein;NEFM;NEF3, NFM; NEFM NEF3, NF-M, NFM neurofilament medium neurofilament medium polypeptide|160 kDa neurofilament protein|neurofilament 3|neurofilament triplet M protein|neurofilament, medium polypeptide 150kDa|neurofilament-3 (150 kD medium)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NEFM, check out the NEFM Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NEFM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Neurofilament NF-M Antibody (M06821-2)
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Customer Q&As
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1 Customer Q&As for Anti-Neurofilament NF-M Antibody
Question
At what pH are you recommending to use for target retrieval for IHC using M06821-2?
Verified customer
Asked: 2022-02-21
Answer
When we do antigen retrieval using Anti-Neurofilament NF-M Antibody (M06821-2), the buffer is pH 6.0.
Boster Scientific Support
Answered: 2022-03-04
