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Product Info Summary
| SKU: | A11147 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Neudesin NENF Antibody
SKU/Catalog Number
A11147
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Neudesin NENF Antibody (Catalog # A11147). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
NENF antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Neudesin NENF Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11147)
Host
Rabbit
Contents
NENF Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
NENF antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human NENF. The immunogen is located within the last 50 amino acids of NENF.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody is predicted to have no cross-reactivity to ZBED2 or ZBED3.
Reactive Species
A11147 is reactive to NENF in Human, Mouse, Rat
Applications
A11147 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
18856 MW
Background of Neudesin
NENF is a secreted protein that is expressed in neurons but not glial cells of the brain. This protein has neurotrophic activity in primary cultured mouse neurons but not mitogenic activity in primary cultured mouse astrocytes. NENF activated mitogen-activated protein (MAP) and phosphotidylinositol-3 kinase pathways and could be inhibited by pertussis toxin but not receptor tyrosine kinases, suggesting that NENF acts through a Gi/Go-protein-coupled receptor.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
NENF antibody can be used for detection of NENF by Western blot at 1 - 2 μg/mL. Despite its predicted molecular weight of ~20kDa, NENF is observed at 37 kDa in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of NENF in human kidney tissue lysate with NENF antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of NENF in rat kidney tissue with NENF antibody at 10 μg/mL.
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Immunofluorescence of NENF in rat kidney tissue with NENF antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For NENF (Source: Uniprot.org, NCBI)
Gene Name
NENF
Full Name
Neudesin
Weight
18856 MW
Superfamily
cytochrome b5 family
Alternative Names
Cell immortalization-related protein 2; CIR2; CIR2cell growth-inhibiting protein 47; NENF; Neudesin; neuron derived neurotrophic factor; Neuron-derived neurotrophic factor; SCIRP10; SCIRP10-related protein; Secreted protein of unknown function; Spinal cord injury related protein 10; SPUF protein; SPUF; SPUFneudesin NENF CIR2, SCIRP10, SPUF neudesin neurotrophic factor neudesin|SCIRP10-related protein|Spinal cord injury related protein 10|cell growth-inhibiting protein 47|cell immortalization-related protein 2|neuron-derived neurotrophic factor|secreted protein of unknown function
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NENF, check out the NENF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NENF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Neudesin NENF Antibody (A11147)
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