Product Info Summary
SKU: | PA1400 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Fractalkine CX3CL1 Antibody Picoband®
View all CX3CL1/Fractalkine Antibodies
SKU/Catalog Number
PA1400
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Fractalkine CX3CL1 Antibody catalog # PA1400. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Fractalkine CX3CL1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1400)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human CX3CL1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1400 is reactive to CX3CL1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
90-100 kDa
Calculated molecular weight
42203 MW
Background of CX3CL1/Fractalkine
Chemokine (C-X3-C motif) ligand 1, also known as CX3CL1, is a large cytokine protein. It is said to be the only known member of the CX3C chemokine family. It is also commonly known under the names fractalkine (in humans) and neurotactin (in mice). Its gene is mapped to the long arm of chromosome 8. Being the large protein, it has got 373 amino acids. Imai et al. (1997) identified a 7-transmembrane high-affinity receptor (CX3CR1; 601470) for fractalkine and showed that it mediates both the adhesive and migratory functions of fractalkine.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1400 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Anti-CX3CL1 antibody, PA1400, Western blotting
Lane 1: Recombinant Human Fractalkine Protein 10ng
Lane 2: Recombinant Human Fractalkine Protein 5ng
Lane 3: Recombinant Human Fractalkine Protein 2.5ng
Protein Target Info & Infographic
Gene/Protein Information For CX3CL1 (Source: Uniprot.org, NCBI)
Gene Name
CX3CL1
Full Name
Fractalkine
Weight
42203 MW
Superfamily
intercrine delta family
Alternative Names
Fractalkine;C-X3-C motif chemokine 1;CX3C membrane-anchored chemokine;Neurotactin;Small-inducible cytokine D1;Processed fractalkine;CX3CL1;FKN, NTT, SCYD1;A-152E5.2; CX3CL1 ABCD-3, C3Xkine, CXC3, CXC3C, NTN, NTT, SCYD1, fractalkine, neurotactin C-X3-C motif chemokine ligand 1 fractalkine|C-X3-C motif chemokine 1|CX3C membrane-anchored chemokine|chemokine (C-X3-C motif) ligand 1|small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin)|small inducible cytokine subfamily D (Cys-X3-Cys), member-1|small-inducible cytokine D1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CX3CL1, check out the CX3CL1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CX3CL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Fractalkine CX3CL1 Antibody Picoband® (PA1400)
Hello CJ!
PA1400 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Distribution Change of Mast Cells in Human Nasal Polyps
Distribution Change of Mast Cells in Human Nasal Polyps
Distribution Change of Mast Cells in Human Nasal Polyps
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Customer Q&As
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3 Customer Q&As for Anti-Fractalkine CX3CL1 Antibody Picoband®
Question
We are currently using anti-CX3CL1 antibody PA1400 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-17
Answer
The anti-CX3CL1 antibody (PA1400) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-17
Question
We have been able to see staining in human thoracic mammary gland. What should we do? Is anti-CX3CL1 antibody supposed to stain thoracic mammary gland positively?
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
Based on literature thoracic mammary gland does express CX3CL1. Based on Uniprot.org, CX3CL1 is expressed in thoracic mammary gland, brain, cerebrospinal fluid, among other tissues. Regarding which tissues have CX3CL1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Cerebrospinal fluid, Pubmed ID: 19838169
Boster Scientific Support
Answered: 2020-03-30
Question
My colleagues were happy with the WB result of your anti-CX3CL1 antibody. However we have observed positive staining in thoracic mammary gland cell membrane using this antibody. Is that expected? Could you tell me where is CX3CL1 supposed to be expressed?
C. Krishna
Verified customer
Asked: 2014-11-27
Answer
Based on literature, thoracic mammary gland does express CX3CL1. Generally CX3CL1 expresses in cell membrane. Regarding which tissues have CX3CL1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Cerebrospinal fluid, Pubmed ID: 19838169
Boster Scientific Support
Answered: 2014-11-27