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Product Info Summary
| SKU: | PB10091 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-PVRL4/NECTIN4 Antibody Picoband®
View all Nectin-4/PVRL4 Antibodies
SKU/Catalog Number
PB10091
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PVRL4/NECTIN4 Antibody Picoband® catalog # PB10091. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PVRL4/NECTIN4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10091)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Nectin-4/PVRL4, different from the related mouse sequence by seven amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB10091 is reactive to NECTIN4 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
66 kDa
Calculated molecular weight
55454 MW
Background of Nectin-4/PVRL4
PVRL4, also known as Nectin-4, is expressed in human skin, hair follicles, and cultured keratinocytes, but not in fibroblasts. This gene encodes a member of the nectin family. The encoded protein contains two immunoglobulin-like (Ig-like) C2-type domains and one Ig-like V-type domain. It is involved in cell adhesion through trans-homophilic and -heterophilic interactions. It is a single-pass type I membrane protein. The soluble form is produced by proteolytic cleavage at the cell surface by the metalloproteinase ADAM17/TACE. The secreted form is found in both breast tumor cell lines and breast tumor patients. Mutations in this gene are the cause of ectodermal dysplasia-syndactyly syndrome type 1, an autosomal recessive disorder. Alternatively spliced transcript variants have been found but the full-length nature of the variant has not been determined.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB10091 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: MCF-7 whole cell, MM453 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Nectin-4/PVRL4 using anti-Nectin-4/PVRL4 antibody (PB10091).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: MCF-7 whole cell lysates,
Lane 2: MM453 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nectin-4/PVRL4 antigen affinity purified polyclonal antibody (Catalog # PB10091) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nectin-4/PVRL4 at approximately 66 kDa. The expected band size for Nectin-4/PVRL4 is at 55 kDa.
Protein Target Info & Infographic
Gene/Protein Information For NECTIN4 (Source: Uniprot.org, NCBI)
Gene Name
NECTIN4
Full Name
Nectin-4
Weight
55454 MW
Superfamily
nectin family
Alternative Names
Nectin-4;Ig superfamily receptor LNIR;Nectin cell adhesion molecule 4 ;Poliovirus receptor-related protein 4;Processed poliovirus receptor-related protein 4;NECTIN4 ;LNIR, PRR4, PVRL4; Nectin4|1200017F15Rik, P, Prr4, Pvrl4|nectin cell adhesion molecule 4|nectin-4|ig superfamily receptor LNIR|nectin 4|poliovirus receptor-related 4|poliovirus receptor-related protein 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NECTIN4, check out the NECTIN4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NECTIN4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PVRL4/NECTIN4 Antibody Picoband® (PB10091)
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Customer Q&As
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4 Customer Q&As for Anti-PVRL4/NECTIN4 Antibody Picoband®
Question
We are currently using anti-PVRL4/NECTIN4 antibody PB10091 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-30
Answer
The anti-PVRL4/NECTIN4 antibody (PB10091) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-30
Question
See attached the WB image, lot number and protocol we used for placenta using anti-PVRL4/NECTIN4 antibody PB10091. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-20
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-PVRL4/NECTIN4 antibody PB10091. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-11-02
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-11-02
Question
Does anti-PVRL4/NECTIN4 antibody PB10091 work for WB with placenta?
F. Gonzalez
Verified customer
Asked: 2013-07-05
Answer
According to the expression profile of placenta, NECTIN4 is highly expressed in placenta. So, it is likely that anti-PVRL4/NECTIN4 antibody PB10091 will work for WB with placenta.
Boster Scientific Support
Answered: 2013-07-05
