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Key=sku, value=A08081-2
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Key=created_at, value=2019-12-19 08:04:01
Key=updated_at, value=2024-10-25 05:02:52
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Key=name, value=Anti-Nectin 2/NECTIN2 Antibody Picoband®
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Key=yoast_keyword_score, value=-1211
Key=yoast_content_score, value=60
Key=badge, value=free-antibody-validation
Key=price, value=370.0000
Key=weight, value=1.0000
Key=meta_title, value=Nectin 2/NECTIN2 Antibody
Key=meta_description, value=Boster Bio Anti-Nectin 2/NECTIN2 Antibody Picoband® catalog # A08081-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat. Supplied as 100μg/vial in Lyophilized form antibody.
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Key=image_label, value=Figure 1. Western blot analysis of Nectin 2/NECTIN2 using anti-Nectin 2/NECTIN2 antibody (A08081-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was lo
Key=small_image_label, value=Figure 1. Western blot analysis of Nectin 2/NECTIN2 using anti-Nectin 2/NECTIN2 antibody (A08081-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was lo
Key=thumbnail_label, value=Figure 1. Western blot analysis of Nectin 2/NECTIN2 using anti-Nectin 2/NECTIN2 antibody (A08081-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was lo
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Key=size, value=100 μg/vial
Key=protein_name, value=Chromaffin granule amine transporter
Key=gene_full_name, value=nectin cell adhesion molecule 2
Key=uniprot_id, value=Q92692
Key=reconstitution, value=Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Key=applications, value=ELISA, Flow Cytometry, IF, IHC, ICC, WB
Key=reactivity, value=Human, Rat
Key=form, value=Lyophilized
Key=concentration, value=Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Key=cross_reactivity, value=No cross-reactivity with other proteins.
Key=recommended_detection_systems, value=Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
Key=source_company, value=Boster A08081-2
Key=isotype, value=Rabbit IgG
Key=molecular_weight, value=17136 MW
Key=sample_size_available, value=30ug for $99, contact us for details
Key=related_product_skus, value=B0001-5,AR1156,AR0146
Key=product_category, value=Primary Antibodies
Key=research_category, value=Atherosclerosis, Cardiovascular, Chaperones, Heat Shock Proteins, Protein Trafficking, Signal Transduction
Key=clonality, value=Polyclonal
Key=swatch_image, value=/a/0/a08081-2-nectin2-primary-antibodies-wb-testing-1.jpg
Key=rating_value, value=100
Key=review_count, value=5
Key=phospho_site
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Key=yoast_twitter_image, value=/a/0/a08072-2-blvrb-primary-antibodies-wb-testing-1.jpg
Key=cj_related_products, value=Peripheral T-cell Lymphoma:M01385|EK0739^Regulation Of Cyclin-dependent Protein Kinase Activity:EK1960|M08081|PROTQ92692
Key=description, value=Boster Bio Anti-Nectin 2/NECTIN2 Antibody Picoband® catalog # A08081-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key=short_description, value=Boster Bio Anti-Nectin 2/NECTIN2 Antibody Picoband® catalog # A08081-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key=meta_keyword, value=Anti-Nectin 2/NECTIN2 Antibody Picoband®
Key=contents, value=Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Key=background, value=Nectin-2 is a 60 or 65 kDa type I TM glycoprotein that is found on a variety of cell types. It is mapped to 19q13.32. This gene encodes a single-pass type I membrane glycoprotein with two Ig-like C2-type domains and an Ig-like V-type domain. This protein is one of the plasma membrane components of adherens junctions. It also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and it is involved in cell to cell spreading of these viruses. Variations in this gene have been associated with differences in the severity of multiple sclerosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Key=gene_name, value=NECTIN2
Key=synonyms, value=Nectin-2; Herpes virus entry mediator B; Herpesvirus entry mediator B; HveB; Nectin cell adhesion molecule 2; Poliovirus receptor-related protein 2; CD112; NECTIN2; HVEB; PRR2; PVRL2
Key=application_details, value=Western blot, 0.25-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -

Key=subcellular_localization, value=Cell membrane. Single-pass type I membrane protein.
Key=tissue_specificity, value=Ubiquitous.
Key=protein_function, value=Modulator of T-cell signaling. Can be either a costimulator of T-cell function, or a coinhibitor, depending on the receptor it binds to. Upon binding to CD226, stimulates T-cell proliferation and cytokine production, including that of IL2, IL5, IL10, IL13, and IFNG. Upon interaction with PVRIG, inhibits T-cell proliferation. These interactions are competitive. Probable cell adhesion protein. (Microbial infection) Acts as a receptor for herpes simplex virus 1 (HHV-1) mutant Rid1, herpes simplex virus 1 (HHV-2) and pseudorabies virus (PRV).
Key=application_notes, value=Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Key=competitor_equivalent_skus, value=sc 169667
Key=immunogen, value=E.coli-derived human Nectin 2/NECTIN2 recombinant protein (Position: Q32-E348).
Key=purification, value=Immunogen affinity purified.
Key=storage, value=Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Key=custom_attribute_1, value=70-80 kDa
Key=custom_attribute_2, value=WB: human K562 whole cell, human HEK293 whole cell
IHC: human mammary cancer tissue, rat testis tissue
ICC/IF: Hela cell
FCM: HepG2 cell
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Anti-Nectin 2/NECTIN2 Antibody Picoband®

Nectin-2/CD112 antibody

Boster Bio Anti-Nectin 2/NECTIN2 Antibody Picoband® catalog # A08081-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: A08081-2
Size: 100 μg/vial
Reactive Species: Human, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, IF, IHC, ICC, WB

Product Name

Anti-Nectin 2/NECTIN2 Antibody Picoband®

View all Nectin-2/CD112 Antibodies

SKU/Catalog Number

A08081-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Nectin 2/NECTIN2 Antibody Picoband® catalog # A08081-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Nectin 2/NECTIN2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08081-2)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Nectin 2/NECTIN2 recombinant protein (Position: Q32-E348).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A08081-2 is reactive to NECTIN2 in Human, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

70-80 kDa

Calculated molecular weight

17136 MW

Background of Nectin-2/CD112

Nectin-2 is a 60 or 65 kDa type I TM glycoprotein that is found on a variety of cell types. It is mapped to 19q13.32. This gene encodes a single-pass type I membrane glycoprotein with two Ig-like C2-type domains and an Ig-like V-type domain. This protein is one of the plasma membrane components of adherens junctions. It also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and it is involved in cell to cell spreading of these viruses. Variations in this gene have been associated with differences in the severity of multiple sclerosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A08081-2 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -

Positive Control

WB: human K562 whole cell, human HEK293 whole cell
IHC: human mammary cancer tissue, rat testis tissue
ICC/IF: Hela cell
FCM: HepG2 cell

Validation Images & Assay Conditions

Gene/Protein Information For NECTIN2 (Source: Uniprot.org, NCBI)

Gene Name

NECTIN2

Full Name

Nectin-2

Weight

17136 MW

Superfamily

nectin family

Alternative Names

Nectin-2; Herpes virus entry mediator B; Herpesvirus entry mediator B; HveB; Nectin cell adhesion molecule 2; Poliovirus receptor-related protein 2; CD112; NECTIN2; HVEB; PRR2; PVRL2 NECTIN2 CD112, HVEB, PRR2, PVRL2, PVRR2 nectin cell adhesion molecule 2 nectin-2|herpesvirus entry protein B|poliovirus receptor-like 2|poliovirus receptor-related 2 (herpesvirus entry mediator B)

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on NECTIN2, check out the NECTIN2 Infographic

NECTIN2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for NECTIN2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
A08081-2

100μg

$370
A08081-2-10ug

10μg sample (liquid)

$99
A08081-2-Biotin

100 μg Biotin conjugated

$570
A08081-2-Cy3

100 μg Cy3 conjugated

$570
A08081-2-Dylight488

100 μg Dylight488 conjugated

$570
A08081-2-Dylight550

100 μg Dylight550 conjugated

$570
A08081-2-Dylight594

100 μg Dylight594 conjugated

$570
A08081-2-FITC

100 μg FITC conjugated

$570
A08081-2-HRP

100 μg HRP conjugated

$570
A08081-2-APC

100 μg APC conjugated

$670
A08081-2-PE

100 μg PE conjugated

$670
A08081-2-iFluor647

100 μg iFluor647 conjugated

$670
A08081-2-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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A08081-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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