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Product Info Summary
| SKU: | EK1960 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human Nectin-2/CD112 ELISA Kit PicoKine®
View all Nectin-2/CD112 ELISA kits
SKU/Catalog Number
EK1960
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Nectin-2/CD112 ELISA Kit PicoKine® (96 Tests). Quantitate Human NECTIN2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 0.1ng/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Nectin-2/CD112 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1960)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: Q32-G360
Sensitivity
<0.1 ng/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1960 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1960 is reactive to NECTIN2 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK1960 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Nectin-2/CD112
Poliovirus receptor-related 2 (PVRL2), also known as nectin-2 and CD112 (formerly herpesvirus entry mediator B, HVEB), is a human plasma membrane glycoprotein. It is mapped to 19q13.32. This gene encodes a single-pass type I membrane glycoprotein with two Ig-like C2-type domains and an Ig-like V-type domain. This protein is one of the plasma membrane components of adherens junctions. It also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and it is involved in cell to cell spreading of these viruses. Variations in this gene have been associated with differences in the severity of multiple sclerosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1960-CAP | Anti-Human NECTIN2 Pre-coated 96-well strip microplate | 1 |
| EK1960-ST | Human NECTIN2 Standard | 2 vials, 10 ng/tube |
| EK1960-DA | Human NECTIN2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.043 | 0.162 | 0.176 | 0.258 | 0.449 | 0.857 | 1.573 | 2.181 |
Data Example Images
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Human Nectin-2/CD112 PicoKine ELISA Kitstandard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Nectin-2/CD112, Dilution ratio of 1:10, concentration in serum and plasma is 4-10 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Nectin-2/CD112 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 211 | 1599 | 5167 | 264 | 1794 | 5274 |
| Standard deviation | 9.92 | 84.75 | 289.35 | 17.69 | 91.49 | 253.15 |
| CV (%) | 4.7% | 5.3% | 5.6% | 6.7% | 5.1% | 4.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Nectin-2/CD112 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 211 | 194 | 202 | 199 | 201 | 6.18 | 3.0% |
| Sample 2 | 1599 | 1649 | 1794 | 1735 | 1694 | 75.38 | 4.4% |
| Sample 3 | 5167 | 5008 | 5074 | 5608 | 5214 | 234.24 | 4.4% |
Protein Target Info & Infographic
Gene/Protein Information For NECTIN2 (Source: Uniprot.Org, NCBI)
Gene Name
NECTIN2
Full Name
Nectin-2
Weight
57.742kDa
Superfamily
nectin family
Alternative Names
Nectin-2; Herpes virus entry mediator B; Herpesvirus entry mediator B; HveB; Nectin cell adhesion molecule 2; Poliovirus receptor-related protein 2; CD112; NECTIN2; HVEB; PRR2; PVRL2 NECTIN2 CD112, HVEB, PRR2, PVRL2, PVRR2 nectin cell adhesion molecule 2 nectin-2|herpesvirus entry protein B|poliovirus receptor-like 2|poliovirus receptor-related 2 (herpesvirus entry mediator B)
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on NECTIN2, check out the NECTIN2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for NECTIN2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Nectin-2/CD112 ELISA Kit PicoKine® (EK1960)
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Customer Q&As
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8 Customer Q&As for Human Nectin-2/CD112 ELISA Kit PicoKine®
Question
Q: if the enzyme conjugated CD112 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CD112 antigen?
Verified Customer
Verified customer
Asked: 2020-12-07
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-12-07
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Z. Krishna
Verified customer
Asked: 2020-07-03
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-07-03
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2020-05-09
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-05-09
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2020-02-16
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-02-16
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement CD112 using the EK1960 Human CD112 Picokine® ELISA Kit?
D. Zhao
Verified customer
Asked: 2019-11-24
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2019-11-24
Question
Q: how can I thaw whole blood sample for CD112 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2019-06-20
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CD112 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2019-06-20
Question
Q: how to proceed with the analysis of ELISA data? I have performed CD112 level in serum.
Verified Customer
Verified customer
Asked: 2018-12-05
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-12-05
Question
Q: is it okay to use citrate plasma as samples in Human CD112 Picokine® ELISA Kit (Catalog # EK1960)?
C. Campbell
Verified customer
Asked: 2018-02-01
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of CD112 causing disruption of its protein structure. CD112 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the CD112 ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-02-01
