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Product Info Summary
| SKU: | A05814 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Nuclear receptor coactivator 7 NCOA7 Antibody
SKU/Catalog Number
A05814
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Nuclear receptor coactivator 7 NCOA7 Antibody (Catalog # A05814). Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Nuclear receptor coactivator 7 NCOA7 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05814)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human NCOA7.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05814 is reactive to NCOA7 in Human, Mouse
Reconstitution
Calculated molecular weight
106162 MW
Background of NCOA7
Enhances the transcriptional activities of several nuclear receptors. Involved in the coactivation of different nuclear receptors, such as ESR1, THRB, PPARG and RARA.
Shao W., Mol. Cell. Biol. 22:3358-3372(2002).
Ohira M., Cancer Lett. 197:63-68(2003).
Ota T., Nat. Genet. 36:40-45(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05814 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from LOVO cells, 3T3 cells and RAW264.7 cells, using NCOA7 antibody A05814.
Protein Target Info & Infographic
Gene/Protein Information For NCOA7 (Source: Uniprot.org, NCBI)
Gene Name
NCOA7
Full Name
Nuclear receptor coactivator 7
Weight
106162 MW
Superfamily
OXR1 family
Alternative Names
Nuclear receptor coactivator 7;140 kDa estrogen receptor-associated protein;Estrogen nuclear receptor coactivator 1;NCOA7;ERAP140, ESNA1;Nbla00052, Nbla10993; NCOA7 ERAP140, ESNA1-AS, Nbla00052, Nbla10993, TLDC4, dJ187J11.3, NCOA7 nuclear receptor coactivator 7 nuclear receptor coactivator 7|140 kDa estrogen receptor-associated protein|TBC/LysM-associated domain containing 4|estrogen nuclear receptor coactivator 1|estrogen receptor associated protein 140 kDa|putative protein product of Nbla00052|putative protein product of Nbla10993
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NCOA7, check out the NCOA7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NCOA7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Nuclear receptor coactivator 7 NCOA7 Antibody (A05814)
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No publications found for A05814
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Customer Q&As
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6 Customer Q&As for Anti-Nuclear receptor coactivator 7 NCOA7 Antibody
Question
Is there a BSA free version of anti-NCOA7 antibody A05814 available?
Verified Customer
Verified customer
Asked: 2020-04-01
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-NCOA7 antibody A05814 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-01
Question
Is this A05814 anti-NCOA7 antibody reactive to the isotypes of NCOA7?
Verified Customer
Verified customer
Asked: 2019-11-15
Answer
The immunogen of A05814 anti-NCOA7 antibody is Synthesized peptide derived from internal of human NCOA7. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-15
Question
We are currently using anti-NCOA7 antibody A05814 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2018-05-08
Answer
The anti-NCOA7 antibody (A05814) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-05-08
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-NCOA7 antibody A05814. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-01-02
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-01-02
Question
My question regards to test anti-NCOA7 antibody A05814 on mouse brain for research purposes, then I may be interested in using anti-NCOA7 antibody A05814 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Zhang
Verified customer
Asked: 2017-04-21
Answer
The products we sell, including anti-NCOA7 antibody A05814, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-04-21
Question
Will anti-NCOA7 antibody A05814 work on zebrafish WB with liver?
A. Yang
Verified customer
Asked: 2013-11-04
Answer
Our lab technicians have not tested anti-NCOA7 antibody A05814 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-NCOA7 antibody A05814 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-11-04
