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Product Info Summary
| SKU: | A30474 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, ICC |
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Product info
Product Name
Anti-TNAP2 Antibody
SKU/Catalog Number
A30474
Size
100ul
Form
Liquid
Description
Boster Bio Anti-TNAP2 Antibody (Catalog# A30474). Tested in IHC-P, IF, ICC, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TNAP2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30474)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human TNAP2. Immunogen sequence location: 131-180
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A30474 is reactive to TNFAIP2 in Human, Mouse
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
72661 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30474 is guaranteed for ELISA, IF, IHC-P, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry, 1:100 - 1:300
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:20000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue, using TNAP2 Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunofluorescence analysis of HUVEC cells, using TNAP2 Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For TNFAIP2 (Source: Uniprot.org, NCBI)
Gene Name
TNFAIP2
Full Name
Tumor necrosis factor alpha-induced protein 2
Weight
72661 MW
Superfamily
SEC6 family
Alternative Names
TNFAIP2;Tumor necrosis factor alpha-induced protein 2;TNF alpha-induced protein 2;Primary response gene B94 protein TNFAIP2 B94, EXOC3L3 TNF alpha induced protein 2 tumor necrosis factor alpha-induced protein 2|exocyst complex component 3-like 3|primary response gene B94 protein|tumor necrosis factor, alpha induced protein 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TNFAIP2, check out the TNFAIP2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TNFAIP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TNAP2 Antibody (A30474)
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No publications found for A30474
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-TNAP2 Antibody
Question
We are currently using anti-TNAP2 antibody A30474 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
The anti-TNAP2 antibody (A30474) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-24
Question
Would A30474 anti-TNAP2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-01
Answer
As indicated on the product datasheet, A30474 anti-TNAP2 antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-01
Question
Is a blocking peptide available for product anti-TNAP2 antibody (A30474)?
Verified Customer
Verified customer
Asked: 2019-08-06
Answer
We do provide the blocking peptide for product anti-TNAP2 antibody (A30474). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-08-06
Question
I have a question about product A30474, anti-TNAP2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
S. Johnson
Verified customer
Asked: 2019-04-30
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30474 anti-TNAP2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-04-30
