Product Info Summary
SKU: | A05814-2 |
---|---|
Size: | 80 µl |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-NCOA7 Antibody (N-term)
SKU/Catalog Number
A05814-2
Size
80 µl
Description
Boster Bio Anti-NCOA7 Antibody (N-term) (Catalog # A05814-2). Tested in WB, Flow Cytometry, IHC-P application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-NCOA7 Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05814-2)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This NCOA7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 76-103 amino acids from the N-terminal region of human NCOA7.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05814-2 is reactive to NCOA7 in Human, Mouse
Applications
A05814-2 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
106162 Da
Background of NCOA7
NCOA7 enhances the transcriptional activities of several nuclear receptors. It is involved in the coactivation of different nuclear receptors, such as ESR1, THRB, PPARG and RARA.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:10-1:50
FC: 1:10-1:50
Validation Images & Assay Conditions
Click image to see more details
All lanes : Anti-NCOA7 Antibody (N-term) at 1:1000 dilution
Lane 1: Hela whole cell lysate
Lane 2: human brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 106 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Click image to see more details
NCOA7 Antibody (N-term) western blot analysis in Hela,MDA-MB-453 cell line and mouse kidney tissue lysates (35ug/lane).This demonstrates the NCOA7 antibody detected the NCOA7 protein (arrow).
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Formalin-fixed and paraffin-embedded human brain tissue reacted with NCOA7 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Click image to see more details
NCOA7 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Protein Target Info & Infographic
Gene/Protein Information For NCOA7 (Source: Uniprot.org, NCBI)
Gene Name
NCOA7
Full Name
Nuclear receptor coactivator 7
Weight
106162 Da
Superfamily
OXR1 family
Alternative Names
140 kDa estrogen receptor-associated protein; dJ187J11.3; ERAP140Nbla00052; ESNA1; Estrogen nuclear receptor coactivator 1; estrogen receptor associated protein 140 kDa; FLJ45605; MGC88425; Nbla10993; nuclear receptor coactivator 7; putative protein product of Nbla00052; putative protein product of Nbla10993 NCOA7 ERAP140, ESNA1-AS, Nbla00052, Nbla10993, TLDC4, dJ187J11.3, NCOA7 nuclear receptor coactivator 7 nuclear receptor coactivator 7|140 kDa estrogen receptor-associated protein|TBC/LysM-associated domain containing 4|estrogen nuclear receptor coactivator 1|estrogen receptor associated protein 140 kDa|putative protein product of Nbla00052|putative protein product of Nbla10993
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NCOA7, check out the NCOA7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NCOA7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NCOA7 Antibody (N-term) (A05814-2)
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No publications found for A05814-2
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