Product Info Summary
SKU: | A10870 |
---|---|
Size: | 100 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-AGPAT5/Lpaat Epsilon Antibody
SKU/Catalog Number
A10870
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-AGPAT5/Lpaat Epsilon Antibody (Catalog # A10870). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AGPAT5/Lpaat Epsilon Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10870)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human AGPAT5.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A10870 is reactive to AGPAT5 in Human
Reconstitution
Calculated molecular weight
42072 MW
Background of Agpat5
Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone. Acts on LPA containing saturated or unsaturated fatty acids C15:0-C20:4 at the sn-1 position using C18:1-CoA as the acyl donor. Also acts on lysophosphatidylethanolamine using oleoyl-CoA, but not arachidonoyl-CoA, and lysophosphatidylinositol using arachidonoyl-CoA, but not oleoyl-CoA. Activity toward lysophosphatidylglycerol not detectable.
Wiemann S., Genome Res. 11:422-435(2001).
Ota T., Nat. Genet. 36:40-45(2004).
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10870 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from LOVO cells, using AGPAT5 antibody A10870.
Protein Target Info & Infographic
Gene/Protein Information For AGPAT5 (Source: Uniprot.org, NCBI)
Gene Name
AGPAT5
Full Name
1-acyl-sn-glycerol-3-phosphate acyltransferase epsilon
Weight
42072 MW
Superfamily
1-acyl-sn-glycerol-3-phosphate acyltransferase family
Alternative Names
1-acyl-sn-glycerol-3-phosphate acyltransferase epsilon;2.3.1.51;1-acylglycerol-3-phosphate O-acyltransferase 5;1-AGP acyltransferase 5;1-AGPAT 5;Lysophosphatidic acid acyltransferase epsilon;LPAAT-epsilon;AGPAT5; Agpat5|1110013A05Rik, D8Ertd319, D8Ertd319e|1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon)|1-acyl-sn-glycerol-3-phosphate acyltransferase epsilon|1-AGP acyltransferase 5|1-AGPAT 5|LPAAT-epsilon|lysophosphatidic acid acyltransferase epsilon
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AGPAT5, check out the AGPAT5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AGPAT5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AGPAT5/Lpaat Epsilon Antibody (A10870)
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No publications found for A10870
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Customer Q&As
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3 Customer Q&As for Anti-AGPAT5/Lpaat Epsilon Antibody
Question
Here is the WB image, lot number and protocol we used for brain lung using anti-AGPAT5/Lpaat Epsilon antibody A10870. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-31
Question
We are currently using anti-AGPAT5/Lpaat Epsilon antibody A10870 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2018-01-26
Answer
The anti-AGPAT5/Lpaat Epsilon antibody (A10870) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-01-26
Question
My question regarding product A10870, anti-AGPAT5/Lpaat Epsilon antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
N. Gonzalez
Verified customer
Asked: 2014-09-08
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A10870 anti-AGPAT5/Lpaat Epsilon antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-09-08