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Product Info Summary
| SKU: | M00025 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-MyD88 Monoclonal Antibody
SKU/Catalog Number
M00025
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-MyD88 Monoclonal Antibody catalog # M00025. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MyD88 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00025)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
FED-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human MyD88 Members of the Toll-like receptor (TLR) family, named for the closely related Toll receptor in Drosophila, play a pivotal role in innate immune responses. TLRs recognize conserved motifs found in various pathogens and mediate defense responses. Triggering of the TLR pathway leads to the activation of NF- Kappa B and subsequent regulation of immune and inflammatory genes.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00025 is reactive to MYD88 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
56 kDa
Calculated molecular weight
88068 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00025 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:100
Positive Control
WB: Raji cell
IHC: human breast cancer tissue
ICC/IF: A549 cell
Validation Images & Assay Conditions
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Western blot analysis of MyD88 expression in Raji cell lysate.
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Immunohistochemical analysis of paraffin-embedded human breast cancer, using MyD88 Antibody.
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Immunofluorescent analysis of A549 cells, using MyD88 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For MYD88 (Source: Uniprot.org, NCBI)
Gene Name
MYD88
Full Name
Myeloid differentiation primary response protein MyD88
Weight
88068 MW
Alternative Names
Signal transducer and activator of transcription 3;Acute-phase response factor;STAT3;APRF; MYD88 IMD68D, MYD88 MYD88 innate immune signal transduction adaptor myeloid differentiation primary response protein MyD88|mutant myeloid differentiation primary response 88|myeloid differentiation primary response 88|myeloid differentiation primary response gene (88)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MYD88, check out the MYD88 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MYD88: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MyD88 Monoclonal Antibody (M00025)
Hello CJ!
M00025 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
High glucose induces and activates Toll-like receptor 4 in endothelial cells of diabetic retinopathy
Toll-Like Receptor 4 Prompts Human Breast Cancer Cells Invasiveness via Lipopolysaccharide Stimulation and Is Overexpressed in Patients with Lymph Node Metastasis
Recommended Resources
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-MyD88 Monoclonal Antibody
Question
See attached the WB image, lot number and protocol we used for umbilical cord blood using anti-MyD88 Monoclonal antibody M00025. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-15
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-15
Question
Can you help my question with product M00025, anti-MyD88 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00025 anti-MyD88 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-25
Question
Is there a BSA free version of anti-MyD88 Monoclonal antibody M00025 available?
Verified Customer
Verified customer
Asked: 2019-12-19
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-MyD88 Monoclonal antibody M00025 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-19
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for umbilical cord blood using anti-MyD88 Monoclonal antibody M00025. Let me know if you need anything else.
T. Johnson
Verified customer
Asked: 2019-12-17
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-17
Question
Would anti-MyD88 Monoclonal antibody M00025 work for WB with umbilical cord blood?
Verified Customer
Verified customer
Asked: 2019-09-13
Answer
According to the expression profile of umbilical cord blood, MYD88 is highly expressed in umbilical cord blood. So, it is likely that anti-MyD88 Monoclonal antibody M00025 will work for WB with umbilical cord blood.
Boster Scientific Support
Answered: 2019-09-13
Question
We are interested in using your anti-MyD88 Monoclonal antibody for cellular response to oxidised low-density lipoprotein particle stimulus studies. Has this antibody been tested with western blotting on a549 cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
We appreciate your inquiry. This M00025 anti-MyD88 Monoclonal antibody is validated on a549 cells. It is guaranteed to work for IF, IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-08-22
Question
We have been able to see staining in human dendritic cell. Are there any suggestions? Is anti-MyD88 Monoclonal antibody supposed to stain dendritic cell positively?
Verified Customer
Verified customer
Asked: 2019-08-21
Answer
From what I have seen in literature dendritic cell does express MYD88. From what I have seen in Uniprot.org, MYD88 is expressed in blood, dendritic cell, epidermal carcinoma, umbilical cord blood, pancreas, erythroleukemia, among other tissues. Regarding which tissues have MYD88 expression, here are a few articles citing expression in various tissues:
Dendritic cell, Pubmed ID: 8957090
Epidermal carcinoma, Pubmed ID: 9013863
Erythroleukemia, Pubmed ID: 23186163
Pancreas, Pubmed ID: 15489334
Umbilical cord blood, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-08-21
Question
Does M00025 anti-MyD88 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-12-10
Answer
As indicated on the product datasheet, M00025 anti-MyD88 Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-12-10
Question
We are currently using anti-MyD88 Monoclonal antibody M00025 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-11-06
Answer
The anti-MyD88 Monoclonal antibody (M00025) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-11-06
Question
Is a blocking peptide available for product anti-MyD88 Monoclonal antibody (M00025)?
A. Patel
Verified customer
Asked: 2018-09-21
Answer
We do provide the blocking peptide for product anti-MyD88 Monoclonal antibody (M00025). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-09-21
Question
Is this M00025 anti-MyD88 Monoclonal antibody reactive to the isotypes of MYD88?
B. Collins
Verified customer
Asked: 2018-03-15
Answer
The immunogen of M00025 anti-MyD88 Monoclonal antibody is A synthesized peptide derived from human MyD88 Members of the Toll-like receptor (TLR) family, named for the closely related Toll receptor in Drosophila, play a pivotal role in innate immune responses. TLRs recognize conserved motifs found in various pathogens and mediate defense responses. Triggering of the TLR pathway leads to the activation of NF- Kappa B and subsequent regulation of immune and inflammatory genes. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-03-15
Question
We want to test anti-MyD88 Monoclonal antibody M00025 on human umbilical cord blood for research purposes, then I may be interested in using anti-MyD88 Monoclonal antibody M00025 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-12-04
Answer
The products we sell, including anti-MyD88 Monoclonal antibody M00025, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-12-04
Question
We were satisfied with the WB result of your anti-MyD88 Monoclonal antibody. However we have seen positive staining in pancreas cytoplasm using this antibody. Is that expected? Could you tell me where is MYD88 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-11-15
Answer
From what I have seen in literature, pancreas does express MYD88. Generally MYD88 expresses in cytoplasm. Regarding which tissues have MYD88 expression, here are a few articles citing expression in various tissues:
Dendritic cell, Pubmed ID: 8957090
Epidermal carcinoma, Pubmed ID: 9013863
Erythroleukemia, Pubmed ID: 23186163
Pancreas, Pubmed ID: 15489334
Umbilical cord blood, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2017-11-15
Question
I see that the anti-MyD88 Monoclonal antibody M00025 works with WB, what is the protocol used to produce the result images on the product page?
H. Kulkarni
Verified customer
Asked: 2016-12-30
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-12-30
Question
I was wanting to use your anti-MyD88 Monoclonal antibody for WB for human umbilical cord blood on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human umbilical cord blood identification?
O. Walker
Verified customer
Asked: 2015-04-29
Answer
You can see on the product datasheet, M00025 anti-MyD88 Monoclonal antibody has been tested for IF, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human umbilical cord blood in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-04-29
Question
We have tried in the past anti-MyD88 Monoclonal antibody for IHC on epidermal carcinoma in the past. I am using human, and We are going to use the antibody for IF next. I would like examining epidermal carcinoma as well as erythroleukemia in our next experiment. Do you have any suggestion on which antibody would work the best for IF?
E. Li
Verified customer
Asked: 2013-06-13
Answer
I have checked the website and datasheets of our anti-MyD88 Monoclonal antibody and I see that M00025 has been tested on human in both IHC and IF. Thus M00025 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2013-06-13
