Click image to see more details
Product Info Summary
| SKU: | A00025-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-MyD88 Antibody Picoband®
SKU/Catalog Number
A00025-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MyD88 Antibody Picoband® catalog # A00025-2. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MyD88 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00025-2)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived rat MyD88 recombinant protein (Position: D14-D263).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00025-2 is reactive to Myd88 in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
33 kDa
Calculated molecular weight
134277 MW
Background of MYD88
MYD88 (MYELOID DIFFERENTIATION PRIMARY RESPONSE GENE 88), is a protein that, in humans, is encoded by the MYD88 gene. MyD88 is a key downstream adapter for most Toll-like receptors (TLRs) and interleukin-1 receptors (IL1Rs). And it is mapped on 3p22.2. MYD88 encodes a cytosolic adapter protein that plays a central role in the innate and adaptive immune response. This protein functions as an essential signal transducer in the interleukin-1 and Toll-like receptor signaling pathways. Overexpression of MYD88 caused an increase in the level of transcription from the interleukin-8 promoter. The C-terminal domain of MYD88 has significant sequence similarity to the cytoplasmic domain of IL1RAP. Inhibiting the IL1R-MYD88 pathway in vivo could block the damage from acute inflammation that occurs in response to sterile cell death, and do so in a way that might not compromise tissue repair or host defense against pathogens.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00025-2 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Positive Control
WB: rat liver tissue, rat ovary tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of MyD88 using anti-MyD88 antibody (A00025-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat ovary tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MyD88 antigen affinity purified polyclonal antibody (Catalog # A00025-2) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MyD88 at approximately 33KD. The expected band size for MyD88 is at 33KD.
Protein Target Info & Infographic
Gene/Protein Information For Myd88 (Source: Uniprot.org, NCBI)
Gene Name
Myd88
Full Name
Myeloid differentiation primary response protein MyD88
Weight
134277 MW
Alternative Names
Myeloid differentiation primary response protein MyD88; Myd88; MYD88 IMD68D, MYD88 MYD88 innate immune signal transduction adaptor myeloid differentiation primary response protein MyD88|mutant myeloid differentiation primary response 88|myeloid differentiation primary response 88|myeloid differentiation primary response gene (88)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Myd88, check out the Myd88 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Myd88: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MyD88 Antibody Picoband® (A00025-2)
Hello CJ!
A00025-2 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Therapeutic effects of Saikosapoin D on bleomycininduced pulmonary fibrosis in mice via regulation of IL- 33/ST2 pathway
Puerarin protects brain tissue against cerebral ischemia/reperfusion injury by inhibiting the inflammatory response
Curcumin exerts anti-inflammatory and antioxidative properties in 1-methyl-4-phenylpyridinium ion (MPP(+))-stimulated mesencephalic astrocytes by interference with TLR4 and downstream signaling pathway
High glucose induces and activates Toll-like receptor 4 in endothelial cells of diabetic retinopathy
Toll-Like Receptor 4 Prompts Human Breast Cancer Cells Invasiveness via Lipopolysaccharide Stimulation and Is Overexpressed in Patients with Lymph Node Metastasis
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-MyD88 Antibody Picoband®
Question
Is a blocking peptide available for product anti-MyD88 antibody (A00025-2)?
Verified Customer
Verified customer
Asked: 2020-04-29
Answer
We do provide the blocking peptide for product anti-MyD88 antibody (A00025-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-04-29
Question
Is there a BSA free version of anti-MyD88 antibody A00025-2 available?
Verified Customer
Verified customer
Asked: 2020-04-24
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-MyD88 antibody A00025-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-24
Question
Is this A00025-2 anti-MyD88 antibody reactive to the isotypes of MYD88?
Verified Customer
Verified customer
Asked: 2020-04-02
Answer
The immunogen of A00025-2 anti-MyD88 antibody is E. coli-derived rat MyD88 recombinant protein (Position: D14-D263). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-02
Question
Please see the WB image, lot number and protocol we used for erythroleukemia using anti-MyD88 antibody A00025-2. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-20
Question
I am interested in to test anti-MyD88 antibody A00025-2 on rat erythroleukemia for research purposes, then I may be interested in using anti-MyD88 antibody A00025-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
The products we sell, including anti-MyD88 antibody A00025-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-24
Question
My team were content with the WB result of your anti-MyD88 antibody. However we have observed positive staining in dendritic cell cytoplasm using this antibody. Is that expected? Could you tell me where is MYD88 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-11-15
Answer
Based on literature, dendritic cell does express MYD88. Generally MYD88 expresses in cytoplasm. Regarding which tissues have MYD88 expression, here are a few articles citing expression in various tissues:
Dendritic cell, Pubmed ID: 8957090
Epidermal carcinoma, Pubmed ID: 9013863
Erythroleukemia, Pubmed ID: 23186163
Pancreas, Pubmed ID: 15489334
Umbilical cord blood, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-11-15
Question
Would A00025-2 anti-MyD88 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-11
Answer
You can see on the product datasheet, A00025-2 anti-MyD88 antibody as been validated on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-11
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for erythroleukemia using anti-MyD88 antibody A00025-2. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-12
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-12
Question
We are currently using anti-MyD88 antibody A00025-2 for rat tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says mouse, rat. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-04
Answer
The anti-MyD88 antibody (A00025-2) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-04
Question
We have been able to see staining in rat pancreas. What should we do? Is anti-MyD88 antibody supposed to stain pancreas positively?
Verified Customer
Verified customer
Asked: 2018-02-19
Answer
According to literature pancreas does express MYD88. According to Uniprot.org, MYD88 is expressed in blood, dendritic cell, epidermal carcinoma, umbilical cord blood, pancreas, erythroleukemia, among other tissues. Regarding which tissues have MYD88 expression, here are a few articles citing expression in various tissues:
Dendritic cell, Pubmed ID: 8957090
Epidermal carcinoma, Pubmed ID: 9013863
Erythroleukemia, Pubmed ID: 23186163
Pancreas, Pubmed ID: 15489334
Umbilical cord blood, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2018-02-19
Question
We ordered your anti-MyD88 antibody for WB on erythroleukemia last year. I am using rat, and We intend to use the antibody for ELISA next. you antibody examining erythroleukemia as well as epidermal carcinoma in our next experiment. Could give a recommendation on which antibody would work the best for ELISA?
Verified Customer
Verified customer
Asked: 2017-11-16
Answer
I viewed the website and datasheets of our anti-MyD88 antibody and it appears that A00025-2 has been tested on rat in both WB and ELISA. Thus A00025-2 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-11-16
Question
I was wanting to use your anti-MyD88 antibody for ELISA for rat erythroleukemia on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat erythroleukemia identification?
Verified Customer
Verified customer
Asked: 2017-08-30
Answer
You can see on the product datasheet, A00025-2 anti-MyD88 antibody has been tested for ELISA, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-08-30
Question
I see that the anti-MyD88 antibody A00025-2 works with ELISA, what is the protocol used to produce the result images on the product page?
E. Jha
Verified customer
Asked: 2015-12-31
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-12-31
Question
Would anti-MyD88 antibody A00025-2 work for ELISA with erythroleukemia?
C. Singh
Verified customer
Asked: 2015-08-24
Answer
According to the expression profile of erythroleukemia, MYD88 is highly expressed in erythroleukemia. So, it is likely that anti-MyD88 antibody A00025-2 will work for ELISA with erythroleukemia.
Boster Scientific Support
Answered: 2015-08-24
Question
I have a question about product A00025-2, anti-MyD88 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
V. Krishna
Verified customer
Asked: 2015-08-13
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00025-2 anti-MyD88 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-08-13
