Click image to see more details
Product Info Summary
| SKU: | A00809-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-LBP Antibody Picoband®
SKU/Catalog Number
A00809-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-LBP Antibody Picoband® catalog # A00809-3. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-LBP Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00809-3)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse LBP recombinant protein (Position: V26-R257).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00809-3 is reactive to Lbp in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
65 kDa
Calculated molecular weight
53384 MW
Background of LBP
Lipopolysaccharide binding protein is a protein that in humans is encoded by the LBP gene. The protein encoded by this gene is involved in the acute-phase immunologic response to gram-negative bacterial infections. Gram-negative bacteria contain a glycolipid, lipopolysaccharide (LPS), on their outer cell wall. Together with bactericidal permeability-increasing protein (BPI), the encoded protein binds LPS and interacts with the CD14 receptor, probably playing a role in regulating LPS-dependent monocyte responses. Studies in mice suggest that the encoded protein is necessary for the rapid acute-phase response to LPS but not for the clearance of LPS from circulation. This protein is part of a family of structurally and functionally related proteins, including BPI, plasma cholesteryl ester transfer protein (CETP), and phospholipid transfer protein (PLTP).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00809-3 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA (Cap), 1-5μg/ml
Positive Control
WB: human Hela whole cell, human Jurkat whole cell, human A549 whole cell, human Raji whole cell, rat liver tissue, mouse liver tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of LBP using anti-LBP antibody (A00809-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human Raji whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: mouse liver tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LBP antigen affinity purified polyclonal antibody (Catalog # A00809-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LBP at approximately 65KD. The expected band size for LBP is at 53KD.
Protein Target Info & Infographic
Gene/Protein Information For Lbp (Source: Uniprot.org, NCBI)
Gene Name
Lbp
Full Name
Lipopolysaccharide-binding protein
Weight
53384 MW
Superfamily
BPI/LBP/Plunc superfamily
Alternative Names
Lipopolysaccharide-binding protein; LBP; Lbp; LBP BPIFD2 lipopolysaccharide binding protein lipopolysaccharide-binding protein|BPI fold containing family D, member 2|LPS-binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Lbp, check out the Lbp Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Lbp: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LBP Antibody Picoband® (A00809-3)
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Customer Q&As
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7 Customer Q&As for Anti-LBP Antibody Picoband®
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-LBP antibody A00809-3. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-04-27
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-27
Question
I was wanting to use your anti-LBP antibody for ELISA for rat liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat liver identification?
Verified Customer
Verified customer
Asked: 2020-02-26
Answer
As indicated on the product datasheet, A00809-3 anti-LBP antibody has been tested for ELISA, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-26
Question
Do you have a BSA free version of anti-LBP antibody A00809-3 available?
Verified Customer
Verified customer
Asked: 2019-09-13
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-LBP antibody A00809-3 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-13
Question
Is this A00809-3 anti-LBP antibody reactive to the isotypes of LBP?
R. Zhang
Verified customer
Asked: 2018-06-15
Answer
The immunogen of A00809-3 anti-LBP antibody is E. coli-derived mouse LBP recombinant protein (Position: V26-R257). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-06-15
Question
Can you help my question with product A00809-3, anti-LBP antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
A. Huang
Verified customer
Asked: 2017-07-26
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00809-3 anti-LBP antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-07-26
Question
We are currently using anti-LBP antibody A00809-3 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?
P. Lewis
Verified customer
Asked: 2016-06-01
Answer
The anti-LBP antibody (A00809-3) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-06-01
Question
Will anti-LBP antibody A00809-3 work for ELISA with liver?
G. Huang
Verified customer
Asked: 2013-04-30
Answer
According to the expression profile of liver, LBP is highly expressed in liver. So, it is likely that anti-LBP antibody A00809-3 will work for ELISA with liver.
Boster Scientific Support
Answered: 2013-04-30
