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Product Info Summary
| SKU: | PA1682 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC, ICC, WB |
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Product info
Product Name
Anti-Protein C/PROC Antibody Picoband®
View all Coagulation Factor XIV/Protein C Antibodies
SKU/Catalog Number
PA1682
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Protein C/PROC Antibody catalog # PA1682. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Protein C/PROC Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1682)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Protein C.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1682 is reactive to PROC in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
36 kDa
Calculated molecular weight
52071 MW
Background of Coagulation Factor XIV/Protein C
Protein C (PROC), also called PC, is a zymogenic (inactive) protein, the activated form of which plays an important role in regulating blood clotting, inflammation, cell death and maintaining the permeability of blood vessel walls in humans and other animals. The PROC gene is mapped on 2q14.3. The PROC gene contains 8 exons and spans about 11 kb by Foster et al. The conversion of protein C to a protease with anticoagulant function by thrombin requires as a cofactor thrombomodulin, an endothelial cell membrane protein. Riewald et al. demonstrated that activated protein C uses the endothelial cell protein C receptor as a coreceptor for cleavage of protease-activated receptor-1 on endothelial cells. Faust et al. demonstrated that the endothelial pathways required for protein C activation are impaired in severe meningococcal sepsis. They stated that improvement in the outcome of children with meningococcal sepsis who were treated with unactivated protein C concentrates had been described in case reports and in 1 uncontrolled series.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1682 is guaranteed for Flow Cytometry, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human
Immunocytochemistry, 0.5-1μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: JURKAT Cell, CEM Cell, SMMC Cell, HELA Cell
IHC: human mammary cancer tissue
FCM: A549 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Protein C using anti-Protein C antibody (PA1682).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: JURKAT Cell Lysate
Lane 2: CEM Cell Lysate
Lane 3: SMMC Cell Lysate
Lane 4: HELA Cell Lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Protein C antigen affinity purified polyclonal antibody (Catalog # PA1682) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Protein C at approximately 36KD. The expected band size for Protein C is at 52KD.
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Figure 2. IHC analysis of Protein C using anti-Protein C antibody (PA1682).
Protein C was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Protein C Antibody (PA1682) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 3. Flow Cytometry analysis of A549 cells using anti-PROC antibody (PA1682).
Overlay histogram showing A549 cells stained with PA1682 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PROC Antibody (PA1682,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For PROC (Source: Uniprot.org, NCBI)
Gene Name
PROC
Full Name
Vitamin K-dependent protein C
Weight
52071 MW
Superfamily
peptidase S1 family
Alternative Names
Vitamin K-dependent protein C;3.4.21.69;Anticoagulant protein C;Autoprothrombin IIA;Blood coagulation factor XIV;Vitamin K-dependent protein C light chain;Vitamin K-dependent protein C heavy chain;Activation peptide;PROC; PROC APC, PC1, THPH3, THPH4, PROC protein C, inactivator of coagulation factors Va and VIIIa vitamin K-dependent protein C|Protein C-Nagoya|activated protein C|anticoagulant protein C|autoprothrombin IIA|blood coagulation factor XIV|prepro-protein C|type I protein C
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PROC, check out the PROC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PROC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Protein C/PROC Antibody Picoband® (PA1682)
Hello CJ!
PA1682 has been cited in 10 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Chen Aq, Liu Zw, Yang Zg, Leng Xj. Comp Biochem Physiol B Biochem Mol Biol. 2012 Sep;163(1):121-8. Doi: 10.1016/J.Cbpb.2012.05.009. Epub 2012 May 18. Characterization Of Bmp15 And Its Regulation By Human Chorionic Gonadotropin In The Follicle Of G...
Zhang S, He B, Goldstein S, Ge J, Wang Z, Ruiz G. Virol J. 2010 Oct 27;7:285. Doi: 10.1186/1743-422X-7-285. The Expression And Significance Of Proto-Oncogene C-Fos In Viral Myocarditis.
Zhang S, Zhang M, Goldstein S, Li Y, Ge J, He B, Ruiz G. Cell Biochem Biophys. 2013 Mar;65(2):249-55. Doi: 10.1007/S12013-012-9428-0. The Effect Of C-Fos On Acute Myocardial Infarction And The Significance Of Metoprolol Intervention In A Rat Model.
Tang Y, Li Y, Yu H, Gao C, Liu L, Chen S, Xing M, Liu L, Yao P. J Nutr Biochem. 2014 Jun;25(6):675-82. Doi: 10.1016/J.Jnutbio.2014.02.009. Epub 2014 Mar 19. Quercetin Prevents Ethanol-Induced Iron Overload By Regulating Hepcidin Through The Bmp6/S...
Bao Hj, Liu Y, Qin Jh, Xu Cs, Hei Nn, Jaber Jr, Chen Qs. Res Vet Sci. 2011 Dec;91(3):E16-24. Doi: 10.1016/J.Rvsc.2011.02.015. Epub 2011 Apr 12. An Immunohistochemical Study Of S-100 Protein In The Intestinal Tract Of Chinese Soft-Shelled Turtle, P...
Ban Jb, Fan Xw, Huang Q, Li Bf, Chen C, Zhang Hc, Xu Sq. Plos One. 2014 May 16;9(5):E97607. Doi: 10.1371/Journal.Pone.0097607. Ecollection 2014. Mono-(2-Ethylhexyl) Phthalate Induces Injury In Human Umbilical Vein Endothelial Cells.
Yang H, Gu J, Zheng Q, Li M, Lian X, Miao J, Jiang J, Wei W. J Biol Chem. 2011 Apr 1;286(13):11865-74. Doi: 10.1074/Jbc.M110.136929. Epub 2011 Feb 10. Rpb5-Mediating Protein Is Required For The Proliferation Of Hepatocellular Carcinoma Cells.
Li Jt, Wang Lf, Zhao Yl, Yang T, Li W, Zhao J, Yu F, Wang L, Meng Yl, Liu Nn, Zhu Xs, Gao Cf, Jia Lt, Yang Ag. Breast Cancer Res. 2014 Oct 14;16(5):454. Doi: 10.1186/S13058-014-0454-2. Nuclear Factor Of Activated T Cells 5 Maintained By Hotair Sup...
Liu R, An L, Liu G, Li X, Tang W, Chen X. Antiviral Res. 2015 Aug;120:101-11. Doi: 10.1016/J.Antiviral.2015.05.008. Epub 2015 May 27. Mouse Lung Slices: An Ex Vivo Model For The Evaluation Of Antiviral And Anti-Inflammatory Agents Against Influenz...
Aksu I, Baykara B, Kiray M, Gurpinar T, Sisman Ar, Ekerbicer N, Tas A, Gokdemir-Yazar O, Uysal N. Biotech Histochem. 2013 May;88(3-4):194-201. Doi: 10.3109/10520295.2012.758311. Epub 2013 Jan 21. Serum Igf-1 Levels Correlate Negatively To Liver Da...
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Customer Q&As
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3 Customer Q&As for Anti-Protein C/PROC Antibody Picoband®
Question
We are currently using anti-Protein C/PROC antibody PA1682 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
The anti-Protein C/PROC antibody (PA1682) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-08
Question
Would PA1682 anti-Protein C/PROC antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-08-27
Answer
As indicated on the product datasheet, PA1682 anti-Protein C/PROC antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-08-27
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-Protein C/PROC antibody PA1682. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-11-30
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-11-30
