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Product Info Summary
| SKU: | M01683 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC |
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Product info
Product Name
Anti-MUC18 / CD146 / MCAM (Melanoma Cell Adhesion Molecule) Monoclonal Antibody
View all MCAM/CD146 Antibodies
SKU/Catalog Number
M01683
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-MUC18 / CD146 / MCAM (Melanoma Cell Adhesion Molecule) Monoclonal Antibody (Catalog # M01683). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-MUC18 / CD146 / MCAM (Melanoma Cell Adhesion Molecule) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01683)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: MUC18/1130
Isotype
IgG1, kappa
Immunogen
Recombinant full-length human MUC18 protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M01683 is reactive to MCAM in Human
Reconstitution
Reconstitute with distilled water.
Observed Molecular Weight
68 kDa
Calculated molecular weight
71607 MW
Background of MCAM/CD146
The human Mel-CAM gene maps to chromosome 11q23 and encodes a trans-membrane glycoprotein, also designated MCAM, MUC 18 or CD146, that belongs to the immunoglobulin superfamily and functions as a Ca2+-independent cell adhesion molecule. Mel-CAM expression is restricted to advanced primary and metastatic melanomas and to cell lines of the neuroectodermal lineage, but not normal melanocytes. Mel-CAM is found on 80% of advanced primary human melanomas and correlates well with development of metastatic disease.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01683 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 8.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded human Melanoma stained with Anti-MUC18 Mouse Monoclonal Antibody (MUC18/1130).
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Formalin-fixed, paraffin-embedded human Melanoma stained with Anti-MUC18 Mouse Monoclonal Antibody (MUC18/1130).
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Formalin-fixed, paraffin-embedded human Tonsil stained with Anti-MUC18 Mouse Monoclonal Antibody (MUC18/1130).
Protein Target Info & Infographic
Gene/Protein Information For MCAM (Source: Uniprot.org, NCBI)
Gene Name
MCAM
Full Name
Cell surface glycoprotein MUC18
Weight
71607 MW
Alternative Names
Cell surface glycoprotein MUC18;Cell surface glycoprotein P1H12;Melanoma cell adhesion molecule;Melanoma-associated antigen A32;Melanoma-associated antigen MUC18;S-endo 1 endothelial-associated antigen;CD146;MCAM;MUC18; MCAM CD146, HEMCAM, METCAM, MUC18, MelCAM melanoma cell adhesion molecule cell surface glycoprotein MUC18|Gicerin|S-endo 1 endothelial-associated |cell surface glycoprotein P1H12|melanoma adhesion molecule|melanoma-associated A32|melanoma-associated MUC18
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MCAM, check out the MCAM Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MCAM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MUC18 / CD146 / MCAM (Melanoma Cell Adhesion Molecule) Monoclonal Antibody (M01683)
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No publications found for M01683
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Customer Q&As
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15 Customer Q&As for Anti-MUC18 / CD146 / MCAM (Melanoma Cell Adhesion Molecule) Monoclonal Antibody
Question
Is a blocking peptide available for product anti-MUC18 / CD146 / MCAM (Melanoma Cell Adhesion Molecule) Monoclonal antibody (M01683)?
Verified Customer
Verified customer
Asked: 2020-01-29
Answer
We do provide the blocking peptide for product anti-MUC18 / CD146 / MCAM (Melanoma Cell Adhesion Molecule) Monoclonal antibody (M01683). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-29
Question
I was wanting to use your anti-MUC18 / CD146 / MCAM (Melanoma Cell Adhesion Molecule) Monoclonal antibody for IHC for human trachea on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human trachea identification?
Verified Customer
Verified customer
Asked: 2019-10-08
Answer
It shows on the product datasheet, M01683 anti-MUC18 / CD146 / MCAM (Melanoma Cell Adhesion Molecule) Monoclonal antibody has been tested for IHC on human tissues. We have an innovator award program that if you test this antibody and show it works in human trachea in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-08
Question
Would M01683 anti-MUC18 / CD146 / MCAM (Melanoma Cell Adhesion Molecule) Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-21
Answer
You can see on the product datasheet, M01683 anti-MUC18 / CD146 / MCAM (Melanoma Cell Adhesion Molecule) Monoclonal antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-21
Question
I see that the anti-MUC18 / CD146 / MCAM (Melanoma Cell Adhesion Molecule) Monoclonal antibody M01683 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-08-12
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for trachea using anti-MUC18 / CD146 / MCAM (Melanoma Cell Adhesion Molecule) Monoclonal antibody M01683. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-28
Question
Would anti-MUC18 / CD146 / MCAM (Melanoma Cell Adhesion Molecule) Monoclonal antibody M01683 work on canine IHC with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2019-06-03
Answer
Our lab technicians have not validated anti-MUC18 / CD146 / MCAM (Melanoma Cell Adhesion Molecule) Monoclonal antibody M01683 on canine. You can run a BLAST between canine and the immunogen sequence of anti-MUC18 / CD146 / MCAM (Melanoma Cell Adhesion Molecule) Monoclonal antibody M01683 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine cervix carcinoma in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-03
Question
Will anti-MUC18 / CD146 / MCAM (Melanoma Cell Adhesion Molecule) Monoclonal antibody M01683 work for IHC with trachea?
Verified Customer
Verified customer
Asked: 2019-04-17
Answer
According to the expression profile of trachea, MCAM is highly expressed in trachea. So, it is likely that anti-MUC18 / CD146 / MCAM (Melanoma Cell Adhesion Molecule) Monoclonal antibody M01683 will work for IHC with trachea.
Boster Scientific Support
Answered: 2019-04-17
Question
Our lab were well pleased with the WB result of your anti-MUC18 / CD146 / MCAM (Melanoma Cell Adhesion Molecule) Monoclonal antibody. However we have been able to see positive staining in trachea membrane using this antibody. Is that expected? Could you tell me where is MCAM supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-02-07
Answer
From literature, trachea does express MCAM. Generally MCAM expresses in membrane. Regarding which tissues have MCAM expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Liver, Pubmed ID: 19159218
Melanoma, Pubmed ID: 2602381, 8378324
PNS, Pubmed ID: 15489334
Trachea, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-02-07
Question
We have been able to see staining in human cervix carcinoma. Any tips? Is anti-MUC18 / CD146 / MCAM (Melanoma Cell Adhesion Molecule) Monoclonal antibody supposed to stain cervix carcinoma positively?
Verified Customer
Verified customer
Asked: 2017-07-14
Answer
From literature cervix carcinoma does express MCAM. From Uniprot.org, MCAM is expressed in popliteal artery, melanoma, trachea, aortic endothelium, pns, cervix carcinoma, liver, among other tissues. Regarding which tissues have MCAM expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Liver, Pubmed ID: 19159218
Melanoma, Pubmed ID: 2602381, 8378324
PNS, Pubmed ID: 15489334
Trachea, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2017-07-14
Question
We are currently using anti-MUC18 / CD146 / MCAM (Melanoma Cell Adhesion Molecule) Monoclonal antibody M01683 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2017-05-26
Answer
The anti-MUC18 / CD146 / MCAM (Melanoma Cell Adhesion Molecule) Monoclonal antibody (M01683) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-05-26
Question
My question regarding product M01683, anti-MUC18 / CD146 / MCAM (Melanoma Cell Adhesion Molecule) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-05-19
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01683 anti-MUC18 / CD146 / MCAM (Melanoma Cell Adhesion Molecule) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-05-19
Question
My lab would like to test anti-MUC18 / CD146 / MCAM (Melanoma Cell Adhesion Molecule) Monoclonal antibody M01683 on human trachea for research purposes, then I may be interested in using anti-MUC18 / CD146 / MCAM (Melanoma Cell Adhesion Molecule) Monoclonal antibody M01683 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
E. Zhang
Verified customer
Asked: 2016-12-22
Answer
The products we sell, including anti-MUC18 / CD146 / MCAM (Melanoma Cell Adhesion Molecule) Monoclonal antibody M01683, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-12-22
Question
See below the WB image, lot number and protocol we used for trachea using anti-MUC18 / CD146 / MCAM (Melanoma Cell Adhesion Molecule) Monoclonal antibody M01683. Please let me know if you require anything else.
E. Jones
Verified customer
Asked: 2016-05-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-05-03
Question
Is this M01683 anti-MUC18 / CD146 / MCAM (Melanoma Cell Adhesion Molecule) Monoclonal antibody reactive to the isotypes of MCAM?
V. Jones
Verified customer
Asked: 2015-11-04
Answer
The immunogen of M01683 anti-MUC18 / CD146 / MCAM (Melanoma Cell Adhesion Molecule) Monoclonal antibody is Recombinant human MUC18 protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-11-04
Question
Do you have a BSA free version of anti-MUC18 / CD146 / MCAM (Melanoma Cell Adhesion Molecule) Monoclonal antibody M01683 available?
R. Wu
Verified customer
Asked: 2015-09-28
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MUC18 / CD146 / MCAM (Melanoma Cell Adhesion Molecule) Monoclonal antibody M01683 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-09-28
