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Product Info Summary
| SKU: | A01530-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-ROBO1 Antibody Picoband®
SKU/Catalog Number
A01530-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ROBO1 Antibody Picoband® catalog # A01530-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ROBO1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01530-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human ROBO1 recombinant protein (Position: F264-P897).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01530-2 is reactive to ROBO1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
250 kDa
Calculated molecular weight
180,930 MW
Background of ROBO1
Roundabout homolog 1 is a protein that in humans is encoded by the ROBO1 gene. Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01530-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Flow Cytometry (Fixed), 1-3μg/1x106 cells
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human HepG2 whole cell, human Hela whole cell, human 293T whole cell, rat brain tissue, mouse brain tissue
FCM: A549 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ROBO1 using anti-ROBO1 antibody (A01530-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human Jurkat whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROBO1 antigen affinity purified polyclonal antibody (Catalog # A01530-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ROBO1 at approximately 250 kDa. The expected band size for ROBO1 is at 181 kDa.
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Figure 2. Flow Cytometry analysis of A549 cells using anti-ROBO1 antibody (A01530-2).
Overlay histogram showing A549 cells stained with A01530-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ROBO1 Antibody (A01530-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For ROBO1 (Source: Uniprot.org, NCBI)
Gene Name
ROBO1
Full Name
Roundabout homolog 1
Weight
180,930 MW
Superfamily
immunoglobulin superfamily
Alternative Names
Roundabout homolog 1; Deleted in U twenty twenty; H-Robo-1; ROBO1; DUTT1 ROBO1 DUTT1, SAX3 roundabout guidance receptor 1 roundabout homolog 1|deleted in U twenty twenty|roundabout, axon guidance receptor, homolog 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ROBO1, check out the ROBO1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ROBO1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ROBO1 Antibody Picoband® (A01530-2)
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Customer Q&As
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1 Customer Q&As for Anti-ROBO1 Antibody Picoband®
Question
We are currently using anti-ROBO1 antibody A01530-2 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
The anti-ROBO1 antibody (A01530-2) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-16
