Product Info Summary
SKU: | A01683 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-CD146/Mcam Antibody Picoband™
View all MCAM/CD146 Antibodies
SKU/Catalog Number
A01683
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD146/Mcam Antibody Picoband™ catalog # A01683. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD146/Mcam Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01683)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse CD146/Mcam recombinant protein (Position: E27-E621).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01683 is reactive to MCAM in Mouse, Rat
Applications
A01683 is guaranteed for ELISA, Flow Cytometry, IF, IHC, WB Boster Guarantee
Observed Molecular Weight
120 kDa
Calculated molecular weight
50514 MW
Background of MCAM/CD146
In humans, the CD146 protein is encoded by the MCAM gene. It is mapped to 9; 9 A5.1. CD146 (cluster of differentiation 146) also known as the melanoma cell adhesion molecule (MCAM) or cell surface glycoprotein MUC18, is a 113kDa cell adhesion molecule currently used as a marker for endothelial cell lineage. MCAM functions as a receptor for laminin alpha 4, a matrix molecule that is broadly expressed within the vascular wall. Accordingly, MCAM is highly expressed by cells that are components of the blood vessel wall, including vascular endothelial cells, smooth muscle cells and pericytes. Its function is still poorly understood, but evidence points to it being part of the endothelial junction associated with the actin cytoskeleton. A member of the Immunoglobulin superfamily, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. It is expressed on chicken embryonic spleen and thymus, activated human T cells, endothelial progenitors such as angioblasts and mesenchymal stem cells, and strongly expressed on blood vessel endothelium and smooth muscle.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat
Immunofluorescence, 2μg/ml, Mouse
Flow Cytometry, 1-3μg/1x106 cells, Mouse, Rat
Direct ELISA, 0.1-0.5μg/ml, Mouse
Validation Images & Assay Conditions
![a01683 mcam primary antibodies wb testing 1 a01683 mcam primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a01683-mcam-primary-antibodies-wb-testing-1.jpg)
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Figure 1. Western blot analysis of MCAM using anti-MCAM antibody (A01683).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat lung tissue lysates,
Lane 2: rat ovary tissue lysates,
Lane 3: rat PC-12 whole cell lysates,
Lane 4: mouse lung tissue lysates,
Lane 5: mouse spleen tissue lysates,
Lane 6: mouse thymus tissue lysates,
Lane 7: mouse kidney tissue lysates,
Lane 8: mouse ovary tissue lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCAM antigen affinity purified polyclonal antibody (Catalog # A01683) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCAM at approximately 120KD. The expected band size for MCAM is at 120KD.
![a01683 mcam primary antibodies ihc testing 2 a01683 mcam primary antibodies ihc testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a01683-mcam-primary-antibodies-ihc-testing-2.jpg)
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Figure 2. IHC analysis of MCAM using anti-MCAM antibody (A01683).
MCAM was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MCAM Antibody (A01683) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![a01683 mcam primary antibodies ihc testing 3 a01683 mcam primary antibodies ihc testing 3](https://www.bosterbio.com/media/catalog/product/a/0/a01683-mcam-primary-antibodies-ihc-testing-3.jpg)
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Figure 3. IHC analysis of MCAM using anti-MCAM antibody (A01683).
MCAM was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MCAM Antibody (A01683) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![a01683 mcam primary antibodies ihc testing 4 a01683 mcam primary antibodies ihc testing 4](https://www.bosterbio.com/media/catalog/product/a/0/a01683-mcam-primary-antibodies-ihc-testing-4.jpg)
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Figure 4. IHC analysis of MCAM using anti-MCAM antibody (A01683).
MCAM was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MCAM Antibody (A01683) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![a01683 mcam primary antibodies if testing 5 a01683 mcam primary antibodies if testing 5](https://www.bosterbio.com/media/catalog/product/a/0/a01683-mcam-primary-antibodies-if-testing-5.jpg)
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Figure 5. IF analysis of MCAM using anti-MCAM antibody (A01683).
MCAM was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-MCAM Antibody (A01683) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
![a01683 mcam primary antibodies fc testing 6 a01683 mcam primary antibodies fc testing 6](https://www.bosterbio.com/media/catalog/product/a/0/a01683-mcam-primary-antibodies-fc-testing-6.png)
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Figure 6. Flow Cytometry analysis of C6 cells using anti-MCAM antibody (A01683).
Overlay histogram showing C6 cells stained with A01683 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MCAM Antibody (A01683, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
![a01683 mcam primary antibodies fc testing 7 a01683 mcam primary antibodies fc testing 7](https://www.bosterbio.com/media/catalog/product/a/0/a01683-mcam-primary-antibodies-fc-testing-7.png)
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Figure 7. Flow Cytometry analysis of Neuro-2a cells using anti-MCAM antibody (A01683).
Overlay histogram showing Neuro-2a cells stained with A01683 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MCAM Antibody (A01683, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For MCAM (Source: Uniprot.org, NCBI)
Gene Name
MCAM
Full Name
Cell surface glycoprotein MUC18
Weight
50514 MW
Alternative Names
CD146 antigen; CD146; cell surface glycoprotein MUC18; Cell surface glycoprotein P1H12; L-Gicerin; MCAM; melanoma adhesion molecule; melanoma cell adhesion moleculeS-endo 1 endothelial-associated antigen; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; MUC18; MUC18Gicerin MCAM CD146, HEMCAM, METCAM, MUC18, MelCAM melanoma cell adhesion molecule cell surface glycoprotein MUC18|Gicerin|S-endo 1 endothelial-associated antigen|cell surface glycoprotein P1H12|melanoma adhesion molecule|melanoma-associated antigen A32|melanoma-associated antigen MUC18
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MCAM, check out the MCAM Infographic
![MCAM infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MCAM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD146/Mcam Antibody Picoband™ (A01683)
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