Click image to see more details
Product Info Summary
| SKU: | M00286-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-MMP2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00286-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-MMP2 Rabbit Monoclonal Antibody catalog # M00286-1. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MMP2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00286-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
BBA-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human MMP2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00286-1 is reactive to MMP2 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
95 kDa
Calculated molecular weight
73882 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00286-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:1000
IHC 1:50-1:200
Positive Control
WB: human HepG2 whole cell
IHC: human breast adenocarcinoma tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MMP2 using anti-MMP2 antibody (M00286-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP2 antigen affinity purified monoclonal antibody (Catalog # M00286-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMP2 at approximately 95 kDa. The expected band size for MMP2 is at 74 kDa.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human breast adenocarcinoma, using MMP2 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For MMP2 (Source: Uniprot.org, NCBI)
Gene Name
MMP2
Full Name
72 kDa type IV collagenase
Weight
73882 MW
Superfamily
peptidase M10A family
Alternative Names
72 kDa type IV collagenase;3.4.24.24;72 kDa gelatinase;Gelatinase A;Matrix metalloproteinase-2;MMP-2;TBE-1;PEX;MMP2;CLG4A; MMP2 CLG4, CLG4A, MMP-2, MMP-II, MONA, TBE-1 matrix metallopeptidase 2 72 kDa type IV collagenase|collagenase type IV-A|matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)|matrix metalloproteinase-2|matrix metalloproteinase-II|neutrophil gelatinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MMP2, check out the MMP2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MMP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MMP2 Rabbit Monoclonal Antibody (M00286-1)
Hello CJ!
M00286-1 has been cited in 28 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Gao X,Qiao X,Xing X,Huang J,Qian J,Wang Y,Zhang Y,Zhang X,Li M,Cui J,Yang Y.Matrix Stiffness-Upregulated MicroRNA-17-5p Attenuates the Intervention Effects of Metformin on HCC Invasion and Metastasis by Targeting the PTEN/PI3K/Akt Pathway.Front Oncol.2020 Aug 19;10:1563.doi:10.3389/fonc.2020.01563.PMID:32974191;PMCID:PMC7466473.
Species: Human
M00286-1 usage in article: APP:IHC, SAMPLE:HCC CELL, DILUTION:1:50
Gao X,Qiao X,Xing X,Huang J,Qian J,Wang Y,Zhang Y,Zhang X,Li M,Cui J,Yang Y.Matrix Stiffness-Upregulated MicroRNA-17-5p Attenuates the Intervention Effects of Metformin on HCC Invasion and Metastasis by Targeting the PTEN/PI3K/Akt Pathway.Front Oncol.2020
Species: Human
M00286-1 usage in article: APP:IHC, SAMPLE:HCC CELL, DILUTION:1:50
Integrated Transcriptomic and Proteomic Analyses of the Interaction Between Chicken Synovial Fibroblasts and Mycoplasma synoviae. X. Jian,Xiao Chunhong,C. Hongyan,Lu Huijun,Yu Haizhong,Y. Jianfen
Species: Human
M00286-1 usage in article: APP:WB, SAMPLE:MCF-7 CELL, DILUTION:NA
Wang F,Ji S,Wang M,Liu L,Li Q,Jiang F,Cen J,Ji B.HMGB1 promoted P-glycoprotein at the blood-brain barrier in MCAO rats via TLR4/NF-κB signaling pathway.Eur J Pharmacol.2020 Aug 5;880:173189.doi:10.1016/j.ejphar.2020.173189.Epub 2020 May 15.PMID:32417325.
Species: Rat
M00286-1 usage in article: APP:IHC, SAMPLE:BRAIN TISSUE, DILUTION:1:100
Hua, D.,Tang, L.,Wang, W.,Tang, S.,Yu, L.,Zhou, X.,Wang, Q.,Sun, C.,Shi, C.,Luo, W.,Jiang, Z.,Li,H.,Yu, S.,Improved Antiglioblastoma Activity and BBB Permeability by Conjugation of Paclitaxel to a Cell‐Penetrative MMP‐2‐Cleavable Peptide. Adv.Sci.2020,200
Species: Human,Mouse
M00286-1 usage in article: APP:WB, SAMPLE:U87MG CELL AND U251 CELL, DILUTION:1:1000
Xu T,Pan L,Li L,Hu S,Zhou H,Yang C,Yang J,Li H,Liu Y,Meng X,Li J.MicroRNA-708 modulates Hepatic Stellate Cells activation and enhances extracellular matrix accumulation via direct targeting TMEM88. J Cell Mol Med. 2020 Jul;24(13):7127-7140.doi: 10.1111/jc
Species: Human
M00286-1 usage in article: APP:WB, SAMPLE:LX-2 CELLS, DILUTION:1:1000
Cyclophosphamide promotes pulmonary metastasis on mouse lung adenocarcinoma
Anti-endometriosis Mechanism of Jiawei Foshou San Based on Network Pharmacology
Tetrahydrocurcumin induces mesenchymal-epithelial transition and suppresses angiogenesis by targeting HIF-1%u03B1 and autophagy in human osteosarcoma
Inactivation of lysyl oxidase by %u03B2-aminopropionitrile inhibits hypoxia-induced invasion and migration of cervical cancer cells
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-MMP2 Rabbit Monoclonal Antibody
Question
Will anti-MMP2 Rabbit Monoclonal antibody M00286-1 work on horse WB with teratocarcinoma testis?
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
Our lab technicians have not tested anti-MMP2 Rabbit Monoclonal antibody M00286-1 on horse. You can run a BLAST between horse and the immunogen sequence of anti-MMP2 Rabbit Monoclonal antibody M00286-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse teratocarcinoma testis in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-13
Question
See below the WB image, lot number and protocol we used for melanoma using anti-MMP2 Rabbit Monoclonal antibody M00286-1. Please let me know if you require anything else.
K. Zhang
Verified customer
Asked: 2019-11-19
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-19
Question
We have seen staining in mouse teratocarcinoma testis. Are there any suggestions? Is anti-MMP2 Rabbit Monoclonal antibody supposed to stain teratocarcinoma testis positively?
Verified Customer
Verified customer
Asked: 2019-11-18
Answer
Based on literature teratocarcinoma testis does express MMP2. Based on Uniprot.org, MMP2 is expressed in mucosa of stomach, teratocarcinoma testis, brain, melanoma, among other tissues. Regarding which tissues have MMP2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Melanoma, Pubmed ID: 1480041, 1655733, 8646777
Teratocarcinoma, and Testis, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-11-18
Question
My team were satisfied with the WB result of your anti-MMP2 Rabbit Monoclonal antibody. However we have observed positive staining in teratocarcinoma testis isoform 1: secreted using this antibody. Is that expected? Could you tell me where is MMP2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-11-12
Answer
From what I have seen in literature, teratocarcinoma testis does express MMP2. Generally MMP2 expresses in isoform 1: secreted, extracellular space,, isoform 2: cytoplasm. mitochondrion. Regarding which tissues have MMP2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Melanoma, Pubmed ID: 1480041, 1655733, 8646777
Teratocarcinoma, and Testis, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-11-12
Question
Is this M00286-1 anti-MMP2 Rabbit Monoclonal antibody reactive to the isotypes of MMP2?
Verified Customer
Verified customer
Asked: 2019-10-21
Answer
The immunogen of M00286-1 anti-MMP2 Rabbit Monoclonal antibody is A synthesized peptide derived from human MMP2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-21
Question
Do you have a BSA free version of anti-MMP2 Rabbit Monoclonal antibody M00286-1 available?
Verified Customer
Verified customer
Asked: 2019-09-30
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MMP2 Rabbit Monoclonal antibody M00286-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-30
Question
Does M00286-1 anti-MMP2 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-14
Answer
As indicated on the product datasheet, M00286-1 anti-MMP2 Rabbit Monoclonal antibody as been tested on IHC-P. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-14
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for melanoma using anti-MMP2 Rabbit Monoclonal antibody M00286-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-13
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-13
Question
I was wanting to use your anti-MMP2 Rabbit Monoclonal antibody for IHC-P for rat melanoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat melanoma identification?
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
As indicated on the product datasheet, M00286-1 anti-MMP2 Rabbit Monoclonal antibody has been tested for IHC-P, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat melanoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-29
Question
We ordered your anti-MMP2 Rabbit Monoclonal antibody for IHC-P on brain a few months ago. I am using human, and We want to use the antibody for WB next. We are interested in examining brain as well as melanoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2018-06-12
Answer
I looked at the website and datasheets of our anti-MMP2 Rabbit Monoclonal antibody and it seems that M00286-1 has been tested on human in both IHC-P and WB. Thus M00286-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-06-12
Question
Is a blocking peptide available for product anti-MMP2 Rabbit Monoclonal antibody (M00286-1)?
Verified Customer
Verified customer
Asked: 2017-10-12
Answer
We do provide the blocking peptide for product anti-MMP2 Rabbit Monoclonal antibody (M00286-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-10-12
Question
Will anti-MMP2 Rabbit Monoclonal antibody M00286-1 work for IHC-P with melanoma?
Verified Customer
Verified customer
Asked: 2017-09-06
Answer
According to the expression profile of melanoma, MMP2 is highly expressed in melanoma. So, it is likely that anti-MMP2 Rabbit Monoclonal antibody M00286-1 will work for IHC-P with melanoma.
Boster Scientific Support
Answered: 2017-09-06
Question
I was wanting to use to test anti-MMP2 Rabbit Monoclonal antibody M00286-1 on rat melanoma for research purposes, then I may be interested in using anti-MMP2 Rabbit Monoclonal antibody M00286-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-08-22
Answer
The products we sell, including anti-MMP2 Rabbit Monoclonal antibody M00286-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-08-22
Question
Can you help my question with product M00286-1, anti-MMP2 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
C. Zhang
Verified customer
Asked: 2016-06-02
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00286-1 anti-MMP2 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-06-02
Question
We are currently using anti-MMP2 Rabbit Monoclonal antibody M00286-1 for rat tissue, and we are happy with the IHC-P results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?
A. Thomas
Verified customer
Asked: 2016-02-24
Answer
The anti-MMP2 Rabbit Monoclonal antibody (M00286-1) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-02-24
Question
I see that the anti-MMP2 Rabbit Monoclonal antibody M00286-1 works with IHC-P, what is the protocol used to produce the result images on the product page?
K. Collins
Verified customer
Asked: 2013-05-22
Answer
You can find protocols for IHC-P on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-05-22
