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Product Info Summary
| SKU: | M00286 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-MMP2 / Collagenase Type IV A (Tumor Metastasis Marker) Monoclonal Antibody
SKU/Catalog Number
M00286
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-MMP2 / Collagenase Type IV A (Tumor Metastasis Marker) Monoclonal Antibody (Catalog # M00286). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-MMP2 / Collagenase Type IV A (Tumor Metastasis Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00286)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: MMP2/1501
Isotype
IgG1, kappa
Immunogen
Recombinant human MMP2 protein fragment (aa444-575) (exact sequence is proprietary)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00286 is reactive to MMP2 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Calculated molecular weight
73882 MW
Background of MMP-2
This monoclonal antibody recognizes a protein of 72kDa, which is identified as MMP2. The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, Fibronectin, Laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-2 (also designated type IV collagenase) cleaves collagen types IV,V, VII and X and gelatin type I. Activation of MMP-2 secretion requires the Ras signaling pathway.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00286 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot (1-2ug/ml) Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
Click image to see more details
Western Blot of (1) Recombinant MMP2 protein and (2) U87 cell lysate using Anti-MMP2 Monoclonal Antibody (MMP2/1501).
Protein Target Info & Infographic
Gene/Protein Information For MMP2 (Source: Uniprot.org, NCBI)
Gene Name
MMP2
Full Name
72 kDa type IV collagenase
Weight
73882 MW
Superfamily
peptidase M10A family
Alternative Names
72 kDa type IV collagenase;3.4.24.24;72 kDa gelatinase;Gelatinase A;Matrix metalloproteinase-2;MMP-2;TBE-1;PEX;MMP2;CLG4A; MMP2 CLG4, CLG4A, MMP-2, MMP-II, MONA, TBE-1 matrix metallopeptidase 2 72 kDa type IV collagenase|collagenase type IV-A|matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)|matrix metalloproteinase-2|matrix metalloproteinase-II|neutrophil gelatinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MMP2, check out the MMP2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MMP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MMP2 / Collagenase Type IV A (Tumor Metastasis Marker) Monoclonal Antibody (M00286)
Hello CJ!
M00286 has been cited in 18 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Downregulation of miR-21 inhibits the malignant phenotype of pancreatic cancer cells by targeting VHL
5-Hydroxy-4′-Nitro-7-Propionyloxy-Genistein Inhibited Invasion and Metastasis via Inactivating Wnt/β-Catenin Signal Pathway in Human Endometrial Carcinoma Ji Endometrial Cells
Hypoxia-induced microRNA-141 regulates trophoblast apoptosis, invasion, and vascularization by blocking CXCL12β/CXCR2/4 signal transduction
Hua, D.,Tang, L.,Wang, W.,Tang, S.,Yu, L.,Zhou, X.,Wang, Q.,Sun, C.,Shi, C.,Luo, W.,Jiang, Z.,Li,H.,Yu, S.,Improved Antiglioblastoma Activity and BBB Permeability by Conjugation of Paclitaxel to a Cell‐Penetrative MMP‐2‐Cleavable Peptide. Adv.Sci.2020,200
Species: Human,Mouse
M00286 usage in article: APP:WB, SAMPLE:U87MG CELL AND U251 CELL, DILUTION:1:1000
Xu T,Pan L,Li L,Hu S,Zhou H,Yang C,Yang J,Li H,Liu Y,Meng X,Li J.MicroRNA-708 modulates Hepatic Stellate Cells activation and enhances extracellular matrix accumulation via direct targeting TMEM88. J Cell Mol Med. 2020 Jul;24(13):7127-7140.doi: 10.1111/jc
Species: Human
M00286 usage in article: APP:WB, SAMPLE:LX-2 CELLS, DILUTION:1:1000
Tetrahydrocurcumin induces mesenchymal-epithelial transition and suppresses angiogenesis by targeting HIF-1%u03B1 and autophagy in human osteosarcoma
Inactivation of lysyl oxidase by %u03B2-aminopropionitrile inhibits hypoxia-induced invasion and migration of cervical cancer cells
Suppression of A549 cell proliferation and metastasis by calycosin via inhibition of the PKC-?/ERK1/2 pathway: An in vitro investigation
Association of MMP-2 expression and prognosis in osteosarcoma patients
5-Hydroxy-4?-Nitro-7-Propionyloxy-Genistein Inhibited Invasion and Metastasis via Inactivating Wnt/?-Catenin Signal Pathway in Human Endometrial Carcinoma Ji Endometrial Cells
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-MMP2 / Collagenase Type IV A (Tumor Metastasis Marker) Monoclonal Antibody
Question
I was wanting to use your anti-MMP2 / Collagenase Type IV A (Tumor Metastasis Marker) Monoclonal antibody for WB for human teratocarcinoma testis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human teratocarcinoma testis identification?
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
As indicated on the product datasheet, M00286 anti-MMP2 / Collagenase Type IV A (Tumor Metastasis Marker) Monoclonal antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human teratocarcinoma testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-21
Question
Would M00286 anti-MMP2 / Collagenase Type IV A (Tumor Metastasis Marker) Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-23
Answer
It shows on the product datasheet, M00286 anti-MMP2 / Collagenase Type IV A (Tumor Metastasis Marker) Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-23
Question
Do you have a BSA free version of anti-MMP2 / Collagenase Type IV A (Tumor Metastasis Marker) Monoclonal antibody M00286 available?
Verified Customer
Verified customer
Asked: 2019-11-18
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-MMP2 / Collagenase Type IV A (Tumor Metastasis Marker) Monoclonal antibody M00286 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-18
Question
I would like to test anti-MMP2 / Collagenase Type IV A (Tumor Metastasis Marker) Monoclonal antibody M00286 on human teratocarcinoma testis for research purposes, then I may be interested in using anti-MMP2 / Collagenase Type IV A (Tumor Metastasis Marker) Monoclonal antibody M00286 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-11
Answer
The products we sell, including anti-MMP2 / Collagenase Type IV A (Tumor Metastasis Marker) Monoclonal antibody M00286, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-11
Question
We were well pleased with the WB result of your anti-MMP2 / Collagenase Type IV A (Tumor Metastasis Marker) Monoclonal antibody. However we have seen positive staining in melanoma isoform 1: secreted using this antibody. Is that expected? Could you tell me where is MMP2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
From what I have seen in literature, melanoma does express MMP2. Generally MMP2 expresses in isoform 1: secreted, extracellular space,, isoform 2: cytoplasm. mitochondrion. Regarding which tissues have MMP2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Melanoma, Pubmed ID: 1480041, 1655733, 8646777
Teratocarcinoma, and Testis, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-10-23
Question
We are currently using anti-MMP2 / Collagenase Type IV A (Tumor Metastasis Marker) Monoclonal antibody M00286 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
The anti-MMP2 / Collagenase Type IV A (Tumor Metastasis Marker) Monoclonal antibody (M00286) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-08
Question
Here is the WB image, lot number and protocol we used for teratocarcinoma testis using anti-MMP2 / Collagenase Type IV A (Tumor Metastasis Marker) Monoclonal antibody M00286. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-08-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-08-30
Question
I see that the anti-MMP2 / Collagenase Type IV A (Tumor Metastasis Marker) Monoclonal antibody M00286 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-05-09
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-05-09
Question
I am interested in using your anti-MMP2 / Collagenase Type IV A (Tumor Metastasis Marker) Monoclonal antibody for ephrin receptor signaling pathway studies. Has this antibody been tested with western blotting on u87 cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-03-29
Answer
We appreciate your inquiry. This M00286 anti-MMP2 / Collagenase Type IV A (Tumor Metastasis Marker) Monoclonal antibody is tested on u87 cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-03-29
Question
Is a blocking peptide available for product anti-MMP2 / Collagenase Type IV A (Tumor Metastasis Marker) Monoclonal antibody (M00286)?
Verified Customer
Verified customer
Asked: 2017-10-12
Answer
We do provide the blocking peptide for product anti-MMP2 / Collagenase Type IV A (Tumor Metastasis Marker) Monoclonal antibody (M00286). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-10-12
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for teratocarcinoma testis using anti-MMP2 / Collagenase Type IV A (Tumor Metastasis Marker) Monoclonal antibody M00286. Let me know if you need anything else.
V. Jones
Verified customer
Asked: 2017-03-30
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-03-30
Question
My question regarding product M00286, anti-MMP2 / Collagenase Type IV A (Tumor Metastasis Marker) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
S. Miller
Verified customer
Asked: 2016-07-04
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00286 anti-MMP2 / Collagenase Type IV A (Tumor Metastasis Marker) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-07-04
Question
Is this M00286 anti-MMP2 / Collagenase Type IV A (Tumor Metastasis Marker) Monoclonal antibody reactive to the isotypes of MMP2?
B. Jones
Verified customer
Asked: 2015-11-03
Answer
The immunogen of M00286 anti-MMP2 / Collagenase Type IV A (Tumor Metastasis Marker) Monoclonal antibody is Recombinant human MMP2 protein fragment (aa444-575) (exact sequence is proprietary). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-11-03
Question
Will anti-MMP2 / Collagenase Type IV A (Tumor Metastasis Marker) Monoclonal antibody M00286 work for WB with teratocarcinoma testis?
A. Patel
Verified customer
Asked: 2014-05-15
Answer
According to the expression profile of teratocarcinoma testis, MMP2 is highly expressed in teratocarcinoma testis. So, it is likely that anti-MMP2 / Collagenase Type IV A (Tumor Metastasis Marker) Monoclonal antibody M00286 will work for WB with teratocarcinoma testis.
Boster Scientific Support
Answered: 2014-05-15
Question
We have observed staining in human brain. Are there any suggestions? Is anti-MMP2 / Collagenase Type IV A (Tumor Metastasis Marker) Monoclonal antibody supposed to stain brain positively?
J. Collins
Verified customer
Asked: 2014-04-22
Answer
Based on literature brain does express MMP2. Based on Uniprot.org, MMP2 is expressed in mucosa of stomach, teratocarcinoma testis, brain, melanoma, among other tissues. Regarding which tissues have MMP2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Melanoma, Pubmed ID: 1480041, 1655733, 8646777
Teratocarcinoma, and Testis, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2014-04-22
