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Product Info Summary
| SKU: | A01366 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-MICA Antibody
SKU/Catalog Number
A01366
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-MICA Antibody (Catalog # A01366). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
MICA antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-MICA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01366)
Host
Rabbit
Contents
MICA Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
MICA antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human MICA. The immunogen is located within the last 50 amino acids of MICA.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Four alternatively spliced transcript variants have been observed.
Reactive Species
A01366 is reactive to MICA in Human, Mouse
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
42915 MW
Background of MICA
Major histocompatibility complex (MHC) class I proteins are ubiquitously expressed and mediate the recognition of intracellular antigens by cytotoxic T cells. A related family, termed the MHC class I chain-related (MIC) proteins are recognized by NKG2D, a receptor on NK and T cells, and promote anti-tumor activity. MICA, a member of the MIC family, is widely expressed on many tumors, and it is the MICA/NKG2D interaction that is thought to stimulate the anti-tumor reactivity by T lymphocytes. Both MICA and MICB mRNA are widely expressed in normal tissues, with MICA being present in virtually every tissue except the nervous system, suggesting that MIC protein expression may only be one component of the anti-tumor activity of the immune system.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01366 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
MICA antibody can be used for detection of MICA by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of MICA in A-20 cell lysate with MICA antibody at (A) 0.5
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Immunohistochemistry of MICA in human colon tissue with MICA antibody at 10 μg/mL.
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Immunofluorescence of MICA in Human Colon tissue with MICA antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For MICA (Source: Uniprot.org, NCBI)
Gene Name
MICA
Full Name
MHC class I polypeptide-related sequence A
Weight
42915 MW
Superfamily
MHC class I family
Alternative Names
PERB11.1, PERB11.1, MHC class I polypeptide-related sequence A, MIC-A MICA MIC-A, PERB11.1 MHC class I polypeptide-related sequence A MHC class I polypeptide-related sequence A|HLA class I |MHC class I chain-related protein A|MHC class I related chain A|MHC class I related sequence A|major histocompatibility complex class I chain-related protein A|stress inducible class I homolog|truncated MHC class I polypeptide-related sequence A|truncated MICA
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MICA, check out the MICA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MICA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MICA Antibody (A01366)
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