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Product Info Summary
| SKU: | M00591-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IHC, WB |
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Product info
Product Name
Anti-Transferrin Receptor (CD71) TFRC Rabbit Monoclonal Antibody
SKU/Catalog Number
M00591-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Transferrin Receptor (CD71) TFRC Rabbit Monoclonal Antibody catalog # M00591-1. Tested in WB, IHC, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Transferrin Receptor (CD71) TFRC Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00591-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AOAA-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human TFR
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00591-1 is reactive to TFRC in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
95 kDa
Calculated molecular weight
84871 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00591-1 is guaranteed for Flow Cytometry, IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
IP 1:50
FC 1:50
Positive Control
WB: human Hela whole cell, human U-87MG whole cell, rat liver tissue
IHC: human placenta tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CD71 using anti-CD71 antibody (M00591-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human U-87MG whole cell lysates,
Lane 3: rat liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD71 antigen affinity purified monoclonal antibody (Catalog # M00591-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD71 at approximately 95 kDa. The expected band size for CD71 is at 85 kDa.
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Figure 2. IHC analysis of CD71 using anti-CD71 antibody (M00591-1).
CD71 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CD71 Antibody (M00591-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For TFRC (Source: Uniprot.org, NCBI)
Gene Name
TFRC
Full Name
Transferrin receptor protein 1
Weight
84871 MW
Superfamily
peptidase M28 family
Alternative Names
Transferrin receptor protein 1;TR;TfR;TfR1;Trfr;T9;p90;CD71;Transferrin receptor protein 1, serum form;sTfR;TFRC; TFRC CD71, IMD46, T9, TFR, TFR1, TR, TRFR, p90 transferrin receptor transferrin receptor protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TFRC, check out the TFRC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TFRC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Transferrin Receptor (CD71) TFRC Rabbit Monoclonal Antibody (M00591-1)
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