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Product Info Summary
| SKU: | PB9612 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-MICA Antibody Picoband®
SKU/Catalog Number
PB9612
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MICA Antibody Picoband® catalog # PB9612. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MICA Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9612)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human MICA.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9612 is reactive to MICA in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
45 kDa
Calculated molecular weight
42915 MW
Background of MICA
This gene encodes the highly polymorphic major histocompatability complex class I chain-related protein A. The protein product is expressed on the cell surface, although unlike canonical class I molecules it does not seem to associate with beta-2-microglobulin. It is a ligand for the NKG2-D type II integral membrane protein receptor. And the protein functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells. Variations in this gene have been associated with susceptibility to psoriasis 1 and psoriatic arthritis, and the shedding of MICA-related antibodies and ligands is involved in the progression from monoclonal gammopathy of undetermined significance to multiple myeloma. Alternative splicing of this gene results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9612 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human Raji whole cell, human SGC-7901 whole cell, human SW620 whole cell, huamn Jurkat whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MICA using anti-MICA antibody (PB9612).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysates,
Lane 2: human SGC-7901 whole cell lysates,
Lane 3: human SW620 whole cell lysates,
Lane 4: huamn Jurkat whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MICA antigen affinity purified polyclonal antibody (Catalog # PB9612) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MICA at approximately 45 kDa. The expected band size for MICA is at 43 kDa.
Protein Target Info & Infographic
Gene/Protein Information For MICA (Source: Uniprot.org, NCBI)
Gene Name
MICA
Full Name
MHC class I polypeptide-related sequence A
Weight
42915 MW
Superfamily
MHC class I family
Alternative Names
MHC class I polypeptide-related sequence A;MIC-A;MICA ;PERB11.1 ; MICA MIC-A, PERB11.1 MHC class I polypeptide-related sequence A MHC class I polypeptide-related sequence A|HLA class I |MHC class I chain-related protein A|MHC class I related chain A|MHC class I related sequence A|major histocompatibility complex class I chain-related protein A|stress inducible class I homolog|truncated MHC class I polypeptide-related sequence A|truncated MICA
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MICA, check out the MICA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MICA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MICA Antibody Picoband® (PB9612)
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Customer Q&As
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4 Customer Q&As for Anti-MICA Antibody Picoband®
Question
We have seen staining in human embl:cap12057.1}. Do you have any suggestions? Is anti-MICA antibody supposed to stain embl:cap12057.1} positively?
Verified Customer
Verified customer
Asked: 2020-01-31
Answer
From literature embl:cap12057.1} does express MICA. From Uniprot.org, MICA is expressed in bone kidney eco:0000312, embl:aah16929.1, blood eco:0000312, embl:cad91415.1, embl:cap12057.1}, among other tissues. Regarding which tissues have MICA expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 10369917, 15104678
Bone, and Kidney, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-01-31
Question
We are currently using anti-MICA antibody PB9612 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-26
Answer
The anti-MICA antibody (PB9612) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-26
Question
Our lab were satisfied with the WB result of your anti-MICA antibody. However we have seen positive staining in embl:cap12057.1} cell membrane using this antibody. Is that expected? Could you tell me where is MICA supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
From what I have seen in literature, embl:cap12057.1} does express MICA. Generally MICA expresses in cell membrane. Regarding which tissues have MICA expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 10369917, 15104678
Bone, and Kidney, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-06-26
Question
I was wanting to use using your anti-MICA antibody for immunoregulatory interactions between a lymphoid and a non-lymphoid cell studies. Has this antibody been tested with western blotting on a549 whole cell lysate? We would like to see some validation images before ordering.
R. Patel
Verified customer
Asked: 2015-05-06
Answer
We appreciate your inquiry. This PB9612 anti-MICA antibody is tested on a549 whole cell lysate, hela whole cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2015-05-06
