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Product Info Summary
| SKU: | PB9671 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-Mesothelin/MSLN Antibody
View all Mesothelin Antibodies
SKU/Catalog Number
PB9671
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Mesothelin/MSLN Antibody catalog # PB9671. Tested in IHC applications. This antibody reacts with Mouse.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Mesothelin/MSLN Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9671)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Mesothelin recombinant protein (Position: K308-L576). Mouse Mesothelin shares 58.4%% and 89.6% amino acid (aa) sequence identity with human and rat Mesothelin, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9671 is reactive to Msln in Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
54 kDa
Calculated molecular weight
69423 MW
Background of Mesothelin
Mesothelin, also known as MSLN or MPF, is a protein that in humans is encoded by the MSLN gene. This gene is mapped to 16p13.3. This gene encodes a precursor protein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiation factor functions as a cytokine that can stimulate colony formation in bone marrow megakaryocytes. Mesothelian is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9671 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, By Heat
Positive Control
IHC: mouse lung tissue
Validation Images & Assay Conditions
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Figure 1. IHC analysis of Mesothelin using anti-Mesothelin antibody (PB9671).
Mesothelin was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Mesothelin Antibody (PB9671) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Msln (Source: Uniprot.org, NCBI)
Gene Name
Msln
Full Name
Mesothelin
Weight
69423 MW
Superfamily
mesothelin family
Alternative Names
Mesothelin;Pre-pro-megakaryocyte-potentiating factor;Megakaryocyte-potentiating factor;MPF;Mesothelin, cleaved form;Msln;Mes, Mpf; MSLN MPF, SMRP mesothelin mesothelin|CAK1 |megakaryocyte potentiating factor|pre-pro-megakaryocyte-potentiating factor|soluble MPF mesothelin related protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Msln, check out the Msln Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Msln: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Mesothelin/MSLN Antibody (PB9671)
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No publications found for PB9671
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Mesothelin/MSLN Antibody
Question
Will anti-Mesothelin/MSLN antibody PB9671 work for IHC with carcinoma?
Verified Customer
Verified customer
Asked: 2020-03-13
Answer
According to the expression profile of carcinoma, MSLN is highly expressed in carcinoma. So, it is likely that anti-Mesothelin/MSLN antibody PB9671 will work for IHC with carcinoma.
Boster Scientific Support
Answered: 2020-03-13
Question
Is a blocking peptide available for product anti-Mesothelin/MSLN antibody (PB9671)?
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
We do provide the blocking peptide for product anti-Mesothelin/MSLN antibody (PB9671). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-02-10
Question
Will PB9671 anti-Mesothelin/MSLN antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-14
Answer
As indicated on the product datasheet, PB9671 anti-Mesothelin/MSLN antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-14
Question
I see that the anti-Mesothelin/MSLN antibody PB9671 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-14
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-08-14
Question
I am looking for using your anti-Mesothelin/MSLN antibody for r-mmu-381426; regulation of insulin-like growth factor (igf) transport and uptake by insulin-like growth factor binding proteins (igfbps) studies. Has this antibody been tested with western blotting on mouse lung tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
I appreciate your inquiry. This PB9671 anti-Mesothelin/MSLN antibody is tested on mouse lung tissue. It is guaranteed to work for IHC in mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-08-12
Question
Is this PB9671 anti-Mesothelin/MSLN antibody reactive to the isotypes of MSLN?
C. Miller
Verified customer
Asked: 2019-07-17
Answer
The immunogen of PB9671 anti-Mesothelin/MSLN antibody is E.coli-derived mouse Mesothelin recombinant protein (Position: K308-L576). Mouse Mesothelin shares 58.4%% and 89.6% amino acid (aa) sequence identity with human and rat Mesothelin, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-17
Question
We are currently using anti-Mesothelin/MSLN antibody PB9671 for mouse tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says mouse. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-17
Answer
The anti-Mesothelin/MSLN antibody (PB9671) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-17
Question
I was wanting to use your anti-Mesothelin/MSLN antibody for IHC for mouse carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse carcinoma identification?
Verified Customer
Verified customer
Asked: 2018-07-19
Answer
It shows on the product datasheet, PB9671 anti-Mesothelin/MSLN antibody has been validated for IHC on mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-07-19
Question
See below the WB image, lot number and protocol we used for carcinoma using anti-Mesothelin/MSLN antibody PB9671. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-05-23
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-05-23
Question
Our lab want to know about to test anti-Mesothelin/MSLN antibody PB9671 on mouse carcinoma for research purposes, then I may be interested in using anti-Mesothelin/MSLN antibody PB9671 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-10-20
Answer
The products we sell, including anti-Mesothelin/MSLN antibody PB9671, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-10-20
Question
My boss were satisfied with the WB result of your anti-Mesothelin/MSLN antibody. However we have seen positive staining in lung spleen cell membrane using this antibody. Is that expected? Could you tell me where is MSLN supposed to be expressed?
S. Li
Verified customer
Asked: 2016-08-10
Answer
From literature, lung spleen does express MSLN. Generally MSLN expresses in cell membrane. Regarding which tissues have MSLN expression, here are a few articles citing expression in various tissues:
Carcinoma, Pubmed ID: 8552591
Lung, and Spleen, Pubmed ID: 15897581
Pancreas, and Placenta, Pubmed ID: 15489334
Pancreatic cancer, Pubmed ID: 7665620
Pancreatic tumor, Pubmed ID: 8288629
Tear, Pubmed ID: 25946035
Boster Scientific Support
Answered: 2016-08-10
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for carcinoma using anti-Mesothelin/MSLN antibody PB9671. Let me know if you need anything else.
S. Lewis
Verified customer
Asked: 2014-08-27
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-08-27
Question
We have observed staining in mouse carcinoma. Any tips? Is anti-Mesothelin/MSLN antibody supposed to stain carcinoma positively?
T. Mangal
Verified customer
Asked: 2014-07-22
Answer
According to literature carcinoma does express MSLN. According to Uniprot.org, MSLN is expressed in right uterine tube, pancreatic cancer, carcinoma, lung spleen, pancreas placenta, pancreatic tumor, tear, among other tissues. Regarding which tissues have MSLN expression, here are a few articles citing expression in various tissues:
Carcinoma, Pubmed ID: 8552591
Lung, and Spleen, Pubmed ID: 15897581
Pancreas, and Placenta, Pubmed ID: 15489334
Pancreatic cancer, Pubmed ID: 7665620
Pancreatic tumor, Pubmed ID: 8288629
Tear, Pubmed ID: 25946035
Boster Scientific Support
Answered: 2014-07-22
Question
Would anti-Mesothelin/MSLN antibody PB9671 work on canine IHC with lung spleen?
M. Rodriguez
Verified customer
Asked: 2014-06-18
Answer
Our lab technicians have not validated anti-Mesothelin/MSLN antibody PB9671 on canine. You can run a BLAST between canine and the immunogen sequence of anti-Mesothelin/MSLN antibody PB9671 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine lung spleen in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-06-18
Question
Do you have a BSA free version of anti-Mesothelin/MSLN antibody PB9671 available?
T. Krishna
Verified customer
Asked: 2014-03-28
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Mesothelin/MSLN antibody PB9671 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-03-28
Question
Can you help my question with product PB9671, anti-Mesothelin/MSLN antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
P. Wu
Verified customer
Asked: 2013-07-25
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9671 anti-Mesothelin/MSLN antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-07-25
