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Product Info Summary
| SKU: | A03697-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC, IHC-F, ICC, WB |
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Product info
Product Name
Anti-TFPI2 Antibody Picoband®
SKU/Catalog Number
A03697-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TFPI2 Antibody Picoband® catalog # A03697-1. Tested in Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TFPI2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03697-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human TFPI2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03697-1 is reactive to TFPI2 in Human, Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
27 kDa, 35 kDa
Calculated molecular weight
26934 MW
Background of TFPI-2
Tissue factor pathway inhibitor 2, also known as TFPI2, is a human gene which is located at 7q22. It is an important regulator of the extrinsic pathway of blood coagulation through its ability to inhibit factor Xa and factor VIIa-tissue factor activity. After a 22-residue signal peptide, the mature TFPI2 protein contains 213 amino acids with 18 cysteines and 2 canonical N-linked glycosylation sites. The purified recombinant TFPI2 strongly inhibited the amidolytic activities of trypsin and the factor VIIa-tissue factor complex. The latter inhibition was markedly enhanced in the presence of heparin. Mouse TFPI2 mRNA is highly expressed in developing mouse placenta, as in human. And there are also high transcript levels in adult mouse liver and kidney.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03697-1 is guaranteed for Flow Cytometry, IHC, IHC-F, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human
Immunocytochemistry, 0.5-1μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: mouse spleen tissue, HELA whole Cell, human placenta tissue, MCF-7 whole cell
IHC: human placenta tissue
FCM: U87 cell, A549 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of TFPI2 using anti-TFPI2 antibody (A03697-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse spleen tissue lysates,
Lane 2: HELA whole Cell lysates,
Lane 3: human placenta tissue lysates,
Lane 4: MCF-7 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TFPI2 antigen affinity purified polyclonal antibody (Catalog # A03697-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TFPI2 at approximately 27KD, 35KD. The expected band size for TFPI2 is at 27KD.
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Figure 2. IHC analysis of TFPI2 using anti-TFPI2 antibody (A03697-1).
TFPI2 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TFPI2 Antibody (A03697-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 3. Flow Cytometry analysis of U87 cells using anti-TFPI2 antibody (A03697-1).
Overlay histogram showing U87 cells stained with A03697-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TFPI2 Antibody (A03697-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Figure 4. Flow Cytometry analysis of A549 cells using anti-TFPI2 antibody (A03697-1).
Overlay histogram showing A549 cells stained with A03697-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TFPI2 Antibody (A03697-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For TFPI2 (Source: Uniprot.org, NCBI)
Gene Name
TFPI2
Full Name
Tissue factor pathway inhibitor 2
Weight
26934 MW
Alternative Names
Tissue factor pathway inhibitor 2;TFPI-2;Placental protein 5;PP5;TFPI2; TFPI2 PP5, REF1, TFPI-2 tissue factor pathway inhibitor 2 tissue factor pathway inhibitor 2|placental protein 5|retinal pigment epithelium cell factor 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TFPI2, check out the TFPI2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TFPI2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TFPI2 Antibody Picoband® (A03697-1)
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Customer Q&As
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7 Customer Q&As for Anti-TFPI2 Antibody Picoband®
Question
See attached the WB image, lot number and protocol we used for placenta using anti-TFPI2 antibody A03697-1. Please let me know if you require anything else.
A. Huang
Verified customer
Asked: 2020-03-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-06
Question
Is a blocking peptide available for product anti-TFPI2 antibody (A03697-1)?
Verified Customer
Verified customer
Asked: 2019-08-27
Answer
We do provide the blocking peptide for product anti-TFPI2 antibody (A03697-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-08-27
Question
Will A03697-1 anti-TFPI2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-02
Answer
You can see on the product datasheet, A03697-1 anti-TFPI2 antibody as been tested on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-02
Question
I was wanting to use your anti-TFPI2 antibody for ICC for mouse placenta on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse placenta identification?
Verified Customer
Verified customer
Asked: 2019-05-20
Answer
It shows on the product datasheet, A03697-1 anti-TFPI2 antibody has been tested for Flow Cytometry, IHC-P, IHC-F, ICC, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse placenta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-20
Question
I see that the anti-TFPI2 antibody A03697-1 works with ICC, what is the protocol used to produce the result images on the product page?
J. Taylor
Verified customer
Asked: 2019-02-12
Answer
You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-02-12
Question
Do you have a BSA free version of anti-TFPI2 antibody A03697-1 available?
Verified Customer
Verified customer
Asked: 2017-12-01
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-TFPI2 antibody A03697-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-12-01
Question
Does anti-TFPI2 antibody A03697-1 work for ICC with placenta?
Verified Customer
Verified customer
Asked: 2017-06-23
Answer
According to the expression profile of placenta, TFPI2 is highly expressed in placenta. So, it is likely that anti-TFPI2 antibody A03697-1 will work for ICC with placenta.
Boster Scientific Support
Answered: 2017-06-23
