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Product Info Summary
| SKU: | A04584 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-COX17 Antibody
SKU/Catalog Number
A04584
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-COX17 Antibody (Catalog # A04584). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-COX17 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04584)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from N-terminal of human COX17.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04584 is reactive to COX17 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
6915 MW
Background of COX17
Copper chaperone for cytochrome c oxidase (COX). Binds two copper ions and deliver them to the Cu(A) site of COX By similarity.
Amaravadi R., Hum. Genet. 99:329-333(1997).
Punter F.A., Hum. Genet. 107:69-74(2000).
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04584 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
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Western blot analysis of extracts from HeLa cells, HepG2 cells, COLO cells and 293 cells, using COX17 antibody A04584.
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Western blot analysis of extracts from 3T3 cells (Lane 2), using COX17 antiobdy A04584. The lane on the left is treated with synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using COX17 antibody A04584.
Protein Target Info & Infographic
Gene/Protein Information For COX17 (Source: Uniprot.org, NCBI)
Gene Name
COX17
Full Name
Cytochrome c oxidase copper chaperone
Weight
6915 MW
Superfamily
COX17 family
Alternative Names
Cytochrome c oxidase copper chaperone;COX17; COX17 cytochrome c oxidase copper chaperone COX17 cytochrome c oxidase copper chaperone|COX17 cytochrome c oxidase assembly homolog|COX17, cytochrome c oxidase copper chaperone|cytochrome c oxidase 17 copper chaperone|cytochrome c oxidase assembly homolog 17|human homolog of yeast mitochondrial copper recruitment
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on COX17, check out the COX17 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for COX17: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-COX17 Antibody (A04584)
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Customer Q&As
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13 Customer Q&As for Anti-COX17 Antibody
Question
Would A04584 anti-COX17 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-04-27
Answer
It shows on the product datasheet, A04584 anti-COX17 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-27
Question
Would anti-COX17 antibody A04584 work on canine IHC with liver?
Verified Customer
Verified customer
Asked: 2020-04-08
Answer
Our lab technicians have not tested anti-COX17 antibody A04584 on canine. You can run a BLAST between canine and the immunogen sequence of anti-COX17 antibody A04584 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine liver in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-08
Question
I was wanting to use your anti-COX17 antibody for IHC for rat cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat cervix carcinoma erythroleukemia identification?
Verified Customer
Verified customer
Asked: 2020-04-01
Answer
It shows on the product datasheet, A04584 anti-COX17 antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-01
Question
Here is the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-COX17 antibody A04584. Please let me know if you require anything else.
E. Jha
Verified customer
Asked: 2020-02-14
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-14
Question
you antibody to test anti-COX17 antibody A04584 on rat cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-COX17 antibody A04584 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-25
Answer
The products we sell, including anti-COX17 antibody A04584, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-25
Question
Is this A04584 anti-COX17 antibody reactive to the isotypes of COX17?
E. Banerjee
Verified customer
Asked: 2019-10-08
Answer
The immunogen of A04584 anti-COX17 antibody is Synthesized peptide derived from N-terminal of human COX17. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-08
Question
I see that the anti-COX17 antibody A04584 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-04-11
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-04-11
Question
We are currently using anti-COX17 antibody A04584 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?
P. Jones
Verified customer
Asked: 2018-11-26
Answer
The anti-COX17 antibody (A04584) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-11-26
Question
Do you have a BSA free version of anti-COX17 antibody A04584 available?
T. Johnson
Verified customer
Asked: 2018-02-09
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-COX17 antibody A04584 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-02-09
Question
I have a question about product A04584, anti-COX17 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
D. Jackson
Verified customer
Asked: 2018-01-01
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04584 anti-COX17 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-01-01
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-COX17 antibody A04584. Let me know if you need anything else.
D. Johnson
Verified customer
Asked: 2017-08-01
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-08-01
Question
Does anti-COX17 antibody A04584 work for IHC with cervix carcinoma erythroleukemia?
D. Carter
Verified customer
Asked: 2015-07-14
Answer
According to the expression profile of cervix carcinoma erythroleukemia, COX17 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-COX17 antibody A04584 will work for IHC with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2015-07-14
Question
Is a blocking peptide available for product anti-COX17 antibody (A04584)?
M. Edwards
Verified customer
Asked: 2013-02-13
Answer
We do provide the blocking peptide for product anti-COX17 antibody (A04584). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-02-13
