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Product Info Summary
| SKU: | PB9287 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IHC, IHC-F, ICC, WB |
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Product info
Product Name
Anti-Mesothelin/MSLN Antibody Picoband®
View all Mesothelin Antibodies
SKU/Catalog Number
PB9287
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Mesothelin/MSLN Antibody Picoband® catalog # PB9287. Tested in ELISA, Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Mesothelin/MSLN Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9287)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Mesothelin recombinant protein (Position: K306-L576). Human Mesothelin shares 57% and 56% amino acid (aa) sequence identity with mouse and rat Mesothelin, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9287 is reactive to MSLN in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
69 kDa
Calculated molecular weight
68986 MW
Background of Mesothelin
Mesothelin, also known as MSLN or MPF is a protein that in humans is encoded by the MSLN gene. This gene is mapped to 16p13.3. This gene encodes a precursor protein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiation factor functions as a cytokine that can stimulate colony formation in bone marrow megakaryocytes. Mesothelian is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9287 is guaranteed for ELISA, Flow Cytometry, IHC, IHC-F, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA, 0.1-0.5μg/ml, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human
Immunocytochemistry, 0.5-1μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: human Hela whole cell
IHC: Human Lung Cancer tissue
FCM: SiHa cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Mesothelin using anti-Mesothelin antibody (PB9287).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Recombinant Human Mesothelin Protein 0.5ng.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mesothelin antigen affinity purified polyclonal antibody (Catalog # PB9287) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Mesothelin at approximately 43KD. The expected band size for Mesothelin is at 43KD.
Click image to see more details
Figure 2. IHC analysis of Mesothelin using anti-Mesothelin antibody (PB9287).
Mesothelin was detected in paraffin-embedded section of Human Lung Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Mesothelin Antibody (PB9287) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. Flow Cytometry analysis of SiHa cells using anti-Mesothelin antibody (PB9287).
Overlay histogram showing SiHa cells stained with PB9287 (Blue line). Mesothelin cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Mesothelin Antibody (PB9287,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Click image to see more details
Figure 4. Western blot analysis of Mesothelin using anti-Mesothelin antibody (PB9287).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mesothelin antigen affinity purified polyclonal antibody (Catalog # PB9287) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Mesothelin at approximately 69KD. The expected band size for Mesothelin is at 69KD.
Protein Target Info & Infographic
Gene/Protein Information For MSLN (Source: Uniprot.org, NCBI)
Gene Name
MSLN
Full Name
Mesothelin
Weight
68986 MW
Superfamily
mesothelin family
Alternative Names
Mesothelin;CAK1 antigen;Pre-pro-megakaryocyte-potentiating factor;Megakaryocyte-potentiating factor;MPF;Mesothelin, cleaved form;MSLN;MPF; MSLN MPF, SMRP mesothelin mesothelin|CAK1 |megakaryocyte potentiating factor|pre-pro-megakaryocyte-potentiating factor|soluble MPF mesothelin related protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MSLN, check out the MSLN Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MSLN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Mesothelin/MSLN Antibody Picoband® (PB9287)
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Customer Q&As
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16 Customer Q&As for Anti-Mesothelin/MSLN Antibody Picoband®
Question
See attached the WB image, lot number and protocol we used for pancreatic tumor using anti-Mesothelin/MSLN antibody PB9287. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-05-08
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-05-08
Question
I am interested in using your anti-Mesothelin/MSLN antibody for post-translational protein modification studies. Has this antibody been tested with western blotting on lung cancer tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
I appreciate your inquiry. This PB9287 anti-Mesothelin/MSLN antibody is tested on mesothelin cells, human hela, lung cancer tissue, siha cells, hela whole cell lysates. It is guaranteed to work for ELISA, Flow Cytometry, IHC-P, IHC-F, ICC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-03-30
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for pancreatic tumor using anti-Mesothelin/MSLN antibody PB9287. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-06
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-06
Question
My colleagues were content with the WB result of your anti-Mesothelin/MSLN antibody. However we have been able to see positive staining in carcinoma cell membrane using this antibody. Is that expected? Could you tell me where is MSLN supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-01-13
Answer
Based on literature, carcinoma does express MSLN. Generally MSLN expresses in cell membrane. Regarding which tissues have MSLN expression, here are a few articles citing expression in various tissues:
Carcinoma, Pubmed ID: 8552591
Lung, and Spleen, Pubmed ID: 15897581
Pancreas, and Placenta, Pubmed ID: 15489334
Pancreatic cancer, Pubmed ID: 7665620
Pancreatic tumor, Pubmed ID: 8288629
Tear, Pubmed ID: 25946035
Boster Scientific Support
Answered: 2020-01-13
Question
We have observed staining in human right uterine tube. What should we do? Is anti-Mesothelin/MSLN antibody supposed to stain right uterine tube positively?
H. Moore
Verified customer
Asked: 2019-12-03
Answer
Based on literature right uterine tube does express MSLN. Based on Uniprot.org, MSLN is expressed in right uterine tube, pancreatic cancer, carcinoma, lung spleen, pancreas placenta, pancreatic tumor, tear, among other tissues. Regarding which tissues have MSLN expression, here are a few articles citing expression in various tissues:
Carcinoma, Pubmed ID: 8552591
Lung, and Spleen, Pubmed ID: 15897581
Pancreas, and Placenta, Pubmed ID: 15489334
Pancreatic cancer, Pubmed ID: 7665620
Pancreatic tumor, Pubmed ID: 8288629
Tear, Pubmed ID: 25946035
Boster Scientific Support
Answered: 2019-12-03
Question
I see that the anti-Mesothelin/MSLN antibody PB9287 works with ELISA, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-25
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-25
Question
Does anti-Mesothelin/MSLN antibody PB9287 work for ELISA with pancreatic tumor?
Verified Customer
Verified customer
Asked: 2019-10-18
Answer
According to the expression profile of pancreatic tumor, MSLN is highly expressed in pancreatic tumor. So, it is likely that anti-Mesothelin/MSLN antibody PB9287 will work for ELISA with pancreatic tumor.
Boster Scientific Support
Answered: 2019-10-18
Question
Do you have a BSA free version of anti-Mesothelin/MSLN antibody PB9287 available?
Verified Customer
Verified customer
Asked: 2019-09-30
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Mesothelin/MSLN antibody PB9287 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-30
Question
We are currently using anti-Mesothelin/MSLN antibody PB9287 for human tissue, and we are satisfied with the IHC-F results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on dog tissues as well?
R. Lewis
Verified customer
Asked: 2019-06-17
Answer
The anti-Mesothelin/MSLN antibody (PB9287) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-17
Question
Is a blocking peptide available for product anti-Mesothelin/MSLN antibody (PB9287)?
Verified Customer
Verified customer
Asked: 2019-05-28
Answer
We do provide the blocking peptide for product anti-Mesothelin/MSLN antibody (PB9287). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-05-28
Question
Is this PB9287 anti-Mesothelin/MSLN antibody reactive to the isotypes of MSLN?
Verified Customer
Verified customer
Asked: 2019-01-25
Answer
The immunogen of PB9287 anti-Mesothelin/MSLN antibody is E.coli-derived human Mesothelin recombinant protein (Position: K306-L576). Human Mesothelin shares 57% and 56% amino acid (aa) sequence identity with mouse and rat Mesothelin, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-01-25
Question
My question regarding product PB9287, anti-Mesothelin/MSLN antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-02-12
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9287 anti-Mesothelin/MSLN antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-02-12
Question
Does PB9287 anti-Mesothelin/MSLN antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-01-09
Answer
As indicated on the product datasheet, PB9287 anti-Mesothelin/MSLN antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-01-09
Question
We bought anti-Mesothelin/MSLN antibody for ELISA on lung spleen in the past. I am using human, and We are going to use the antibody for WB next. We are interested in examining lung spleen as well as tear in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2018-01-04
Answer
I have checked the website and datasheets of our anti-Mesothelin/MSLN antibody and I see that PB9287 has been validated on human in both ELISA and WB. Thus PB9287 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-01-04
Question
I was wanting to use your anti-Mesothelin/MSLN antibody for ELISA for human pancreatic tumor on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human pancreatic tumor identification?
M. Mangal
Verified customer
Asked: 2017-09-18
Answer
As indicated on the product datasheet, PB9287 anti-Mesothelin/MSLN antibody has been validated for ELISA, Flow Cytometry, IHC-P, IHC-F, ICC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human pancreatic tumor in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-09-18
Question
you antibody to test anti-Mesothelin/MSLN antibody PB9287 on human pancreatic tumor for research purposes, then I may be interested in using anti-Mesothelin/MSLN antibody PB9287 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-08-08
Answer
The products we sell, including anti-Mesothelin/MSLN antibody PB9287, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-08-08
