Human Marapsin/Pancresin ELISA Kit PicoKine®

Marapsin/Pancreasin ELISA kit for Human

Human Marapsin/Pancresin ELISA Kit PicoKine® (96 Tests). Quantitate Human PRSS27 in cell culture supernatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Info Summary

SKU: EK1479
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants and serum.

Product Name

Human Marapsin/Pancresin ELISA Kit PicoKine®

View all Marapsin/Pancreasin ELISA kits

SKU/Catalog Number

EK1479

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Marapsin/Pancresin ELISA Kit PicoKine® (96 Tests). Quantitate Human PRSS27 in cell culture supernatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Marapsin/Pancresin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1479)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: NS0; Immunogen sequence: M35-K290

Sensitivity

<10 pg/ml

Assay Range

125 pg/ml - 8,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

See datasheet of EK1479 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1479 is reactive to PRSS27 in Human samples

Validated Sample Types

cell culture supernatants and serum.

Application Guarantee

EK1479 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Marapsin/Pancreasin

Pancreasin, also known as PRSS27 or Marapsin, is a pancreatic tryptic serine peptidase that cleaves peptides after an arginine residue. It belongs to the group-1 subfamily of serine proteases. The pancreasin gene is mapped to chromosome 16p13.3. Pancreasin is resistant to inactivation by large molecular mass trypsin inhibitors, but it is sensitive to benzamidine and leupeptin.

Kit Components

Catalog Number Description Quantity
EK1479-CAP Anti-Human PRSS27 Pre-coated 96-well strip microplate 1
EK1479-ST Human PRSS27 Standard 2 vials, 10 ng/tube
EK1479-DA Human PRSS27 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)01252505001000200040008000
O.D.0.0290.060.1240.1950.4320.8341.3691.897

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Marapsin/Pancresin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)16713161566216114175245
Standard deviation10.3558.59311.4112.3977.93326.2%9
CV (%)6.2%4.2%5.5%7.7%5.5%6.2%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Marapsin/Pancreasin in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11671761711651694.22.4%
Sample 21395127813521276132550.593.8%
Sample 356625287530860685581318.075.6%
*number of samples for each test n=16.

Gene/Protein Information For PRSS27 (Source: Uniprot.Org, NCBI)

Gene Name

PRSS27

Full Name

Serine protease 27

Weight

31940 MW

Superfamily

peptidase S1 family

Alternative Names

Serine protease 27;3.4.21.-;Marapsin;Pancreasin;PRSS27;MPN;UNQ1884/PRO4327; PRSS27 CAPH2, MPN serine protease 27 serine protease 27|channel-activating protease 2|marapsin|pancreasin|protease, serine 27

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on PRSS27, check out the PRSS27 Infographic

PRSS27 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for PRSS27: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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4 Customer Q&As for Human Marapsin/Pancresin ELISA Kit PicoKine®

Question

Q: which procedure should I follow in order to thaw whole blood sample for Marapsin ELISA after freezing?

P. Baker

Verified customer

Asked: 2018-11-24

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Marapsin for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2018-11-24

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

A. Surve

Verified customer

Asked: 2018-04-05

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-04-05

Question

Q: how to analyze ELISA data? I have performed Marapsin level in serum.

Verified Customer

Verified customer

Asked: 2018-03-06

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-03-06

Question

Q: if the enzyme conjugated Marapsin antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Marapsin antigen?

Verified Customer

Verified customer

Asked: 2016-12-09

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2016-12-09

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