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Product Info Summary
| SKU: | A04564-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-MARK MARK1 Antibody
SKU/Catalog Number
A04564-1
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-MARK MARK1 Antibody (Catalog # A04564-1). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MARK MARK1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04564-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human MARK.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04564-1 is reactive to MARK1 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
89003 MW
Background of MARK1
Serine/threonine-protein kinase involved in cell polarity and microtubule dynamics regulation. Phosphorylates DCX, MAP2, MAP4 and MAPT/TAU. Involved in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly. Involved in the regulation of neuronal migration through its dual activities in regulating cellular polarity and microtubule dynamics, possibly by phosphorylating and regulating DCX. Also acts as a positive regulator of the Wnt signaling pathway, probably by mediating phosphorylation of dishevelled proteins (DVL1, DVL2 and/or DVL3).
Lizcano J.M., EMBO J. 23:833-843(2004).
Nagase T., DNA Res. 7:143-150(2000).
Gregory S.G., Nature 441:315-321(2006).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04564-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from HeLa cells, using MARK antibody A04564-1.
Protein Target Info & Infographic
Gene/Protein Information For MARK1 (Source: Uniprot.org, NCBI)
Gene Name
MARK1
Full Name
Serine/threonine-protein kinase MARK1
Weight
89003 MW
Superfamily
protein kinase superfamily
Alternative Names
Serine/threonine-protein kinase MARK1;2.7.11.1;2.7.11.26;MAP/microtubule affinity-regulating kinase 1;PAR1 homolog c;Par-1c;Par1c;MARK1 ;KIAA1477 , MARK ; MARK1 MARK, Par-1c, Par1c microtubule affinity regulating kinase 1 serine/threonine-protein kinase MARK1|MAP/microtubule affinity-regulating kinase 1|PAR1 homolog c
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MARK1, check out the MARK1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MARK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MARK MARK1 Antibody (A04564-1)
Hello CJ!
No publications found for A04564-1
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Customer Reviews
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Customer Q&As
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13 Customer Q&As for Anti-MARK MARK1 Antibody
Question
Is this A04564-1 anti-MARK antibody reactive to the isotypes of MARK1?
Verified Customer
Verified customer
Asked: 2020-04-03
Answer
The immunogen of A04564-1 anti-MARK antibody is Synthesized peptide derived from internal of human MARK. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-03
Question
Is a blocking peptide available for product anti-MARK antibody (A04564-1)?
Verified Customer
Verified customer
Asked: 2020-02-26
Answer
We do provide the blocking peptide for product anti-MARK antibody (A04564-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-02-26
Question
We are interested in to test anti-MARK antibody A04564-1 on human pubmed:10819331} for research purposes, then I may be interested in using anti-MARK antibody A04564-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
The products we sell, including anti-MARK antibody A04564-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-11
Question
Will A04564-1 anti-MARK antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-05
Answer
It shows on the product datasheet, A04564-1 anti-MARK antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-05
Question
We are currently using anti-MARK antibody A04564-1 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
The anti-MARK antibody (A04564-1) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-22
Question
I was wanting to use your anti-MARK antibody for WB for human pubmed:10819331} on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human pubmed:10819331} identification?
Verified Customer
Verified customer
Asked: 2019-07-26
Answer
It shows on the product datasheet, A04564-1 anti-MARK antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human pubmed:10819331} in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-26
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for pubmed:10819331} using anti-MARK antibody A04564-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-10
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-10
Question
Will anti-MARK antibody A04564-1 work on primate WB with pubmed:10819331}?
Verified Customer
Verified customer
Asked: 2019-03-13
Answer
Our lab technicians have not validated anti-MARK antibody A04564-1 on primate. You can run a BLAST between primate and the immunogen sequence of anti-MARK antibody A04564-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate pubmed:10819331} in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-03-13
Question
I have attached the WB image, lot number and protocol we used for pubmed:10819331} using anti-MARK antibody A04564-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-10-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-10-22
Question
Do you have a BSA free version of anti-MARK antibody A04564-1 available?
A. Mangal
Verified customer
Asked: 2018-05-08
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MARK antibody A04564-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-05-08
Question
I see that the anti-MARK antibody A04564-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-12-28
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-12-28
Question
Can you help my question with product A04564-1, anti-MARK antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-12-05
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04564-1 anti-MARK antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-12-05
Question
Does anti-MARK antibody A04564-1 work for WB with pubmed:10819331}?
C. Li
Verified customer
Asked: 2013-08-13
Answer
According to the expression profile of pubmed:10819331}, MARK1 is highly expressed in pubmed:10819331}. So, it is likely that anti-MARK antibody A04564-1 will work for WB with pubmed:10819331}.
Boster Scientific Support
Answered: 2013-08-13
