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Product Info Summary
| SKU: | PB10103 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-TIAM1 Antibody Picoband®
SKU/Catalog Number
PB10103
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TIAM1 Antibody Picoband® catalog # PB10103. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TIAM1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10103)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human TIAM1, different from the related mouse sequence by five amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB10103 is reactive to TIAM1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
178 kDa
Calculated molecular weight
177508 MW
Background of TIAM1
T-cell lymphoma invasion and metastasis-inducing protein 1 is a protein that in humans is encoded by the TIAM1 gene. This gene is mapped to 21q22.11. TIAM1 modulates the activity of Rho GTP-binding proteins and connects extracellular signals to cytoskeletal activities. In addition, TIAM1 activates Rac1, CDC42, and to a lesser extent RhoA.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB10103 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: MCF-7 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of TIAM1 using anti-TIAM1 antibody (PB10103).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIAM1 antigen affinity purified polyclonal antibody (Catalog # PB10103) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIAM1 at approximately 178 kDa. The expected band size for TIAM1 is at 178 kDa.
Protein Target Info & Infographic
Gene/Protein Information For TIAM1 (Source: Uniprot.org, NCBI)
Gene Name
TIAM1
Full Name
T-lymphoma invasion and metastasis-inducing protein 1
Weight
177508 MW
Superfamily
TIAM family
Alternative Names
T-lymphoma invasion and metastasis-inducing protein 1;TIAM-1;TIAM1; TIAM1 TIAM-1 TIAM Rac1 associated GEF 1 T-lymphoma invasion and metastasis-inducing protein 1|T cell lymphoma invasion and metastasis 1|human T-lymphoma invasion and metastasis inducing TIAM1 protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TIAM1, check out the TIAM1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TIAM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TIAM1 Antibody Picoband® (PB10103)
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Customer Q&As
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4 Customer Q&As for Anti-TIAM1 Antibody Picoband®
Question
I was wanting to use your anti-TIAM1 antibody for WB for human fetal brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human fetal brain identification?
Verified Customer
Verified customer
Asked: 2020-04-10
Answer
As indicated on the product datasheet, PB10103 anti-TIAM1 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human fetal brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-10
Question
Will PB10103 anti-TIAM1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-06
Answer
As indicated on the product datasheet, PB10103 anti-TIAM1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-06
Question
We are currently using anti-TIAM1 antibody PB10103 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on dog tissues as well?
R. Parker
Verified customer
Asked: 2017-03-22
Answer
The anti-TIAM1 antibody (PB10103) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-03-22
Question
Do you have a BSA free version of anti-TIAM1 antibody PB10103 available?
R. Krishna
Verified customer
Asked: 2014-12-26
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TIAM1 antibody PB10103 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-12-26
